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      RY703 Streptavidin
      RY703 Streptavidin

      Flow cytometric analysis of CD3 expression on Human peripheral blood lymphocytes.  Human whole blood was treated with BD Pharm Lyse™ Lysing Buffer (Cat. No. 555899) to lyse erythrocytes. Leukocytes were then either not labeled (dashed line histogram) or labeled (solid line histogram) with Biotin Mouse Anti-Human CD3 antibody (Cat. No. 555331). The cells were then washed and secondarily stained with BD Horizon™ RY703 Streptavidin (Cat. No. 571479) at 0.25 µg/test. The fluorescence histogram showing the expression of CD3 (or unlabeled control staining) was derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. Samples were acquired on the BD FACSymphony™ A5 SE Cell Analyzer and spectrally unmixed with autofluorescence using FlowJo™ v10.10 Software. Data shown on this Technical Data Sheet are not lot specific.

      RY703 Streptavidin

      Flow cytometric analysis of CD45R/B220 expression on Mouse splenic leukocytes.  Mouse splenic leukocytes were either not labeled (dashed line histogram) or labeled (solid line histogram) with Biotin Rat Anti-Mouse CD45R/B220 antibody (Cat. No. 553085/553086). The cells were then washed and secondarily stained with BD Horizon™ RY703 Streptavidin (Cat. No. 571479) at 0.25 µg/test. The fluorescence histogram showing CD45R/B220 expression (or unlabeled control staining) was derived from gated events with the forward and side light-scatter characteristics of viable splenic leukocytes. Samples were acquired on the BD FACSymphony™ A5 SE Cell Analyzer and spectrally unmixed with autofluorescence using FlowJo™ v10.10 Software. Data shown on this Technical Data Sheet are not lot specific.

      RY703 Streptavidin RY703 Streptavidin

      Flow cytometric analysis of CD3 expression on Human peripheral blood lymphocytes.  Human whole blood was treated with BD Pharm Lyse™ Lysing Buffer (Cat. No. 555899) to lyse erythrocytes. Leukocytes were then either not labeled (dashed line histogram) or labeled (solid line histogram) with Biotin Mouse Anti-Human CD3 antibody (Cat. No. 555331). The cells were then washed and secondarily stained with BD Horizon™ RY703 Streptavidin (Cat. No. 571479) at 0.25 µg/test. The fluorescence histogram showing the expression of CD3 (or unlabeled control staining) was derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. Samples were acquired on the BD FACSymphony™ A5 SE Cell Analyzer and spectrally unmixed with autofluorescence using FlowJo™ v10.10 Software. Data shown on this Technical Data Sheet are not lot specific.

      Flow cytometric analysis of CD45R/B220 expression on Mouse splenic leukocytes.  Mouse splenic leukocytes were either not labeled (dashed line histogram) or labeled (solid line histogram) with Biotin Rat Anti-Mouse CD45R/B220 antibody (Cat. No. 553085/553086). The cells were then washed and secondarily stained with BD Horizon™ RY703 Streptavidin (Cat. No. 571479) at 0.25 µg/test. The fluorescence histogram showing CD45R/B220 expression (or unlabeled control staining) was derived from gated events with the forward and side light-scatter characteristics of viable splenic leukocytes. Samples were acquired on the BD FACSymphony™ A5 SE Cell Analyzer and spectrally unmixed with autofluorescence using FlowJo™ v10.10 Software. Data shown on this Technical Data Sheet are not lot specific.

      商品详情
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      BD Horizon™
      Flow cytometry (Routinely Tested)
      0.1 mg/ml
      Aqueous buffered solution containing ≤0.09% sodium azide.
      RUO


      准备和存储

      Streptavidin was conjugated with dye under optimum conditions, and unconjugated Streptavidin and free dye were removed Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
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      商品通知

      1. When using high concentrations of antibody, background binding of this dye to erythroid fragments produced by ammonium chloride-based lysis, such as with BD Pharm Lyse™ Lysing Buffer (Cat. No. 555899), has been observed when the antibody conjugate was present during the lysis procedure. This may cause nonspecific staining of target cells, such as leukocytes, which have bound the resulting erythroid fragments. This background can be mitigated by any of the following: titrating the antibody conjugate to a lower concentration, fixing samples with formaldehyde, or removing erythrocytes before staining (eg, gradient centrifugation or pre-lysis with wash). This background has not been observed when cells were lysed with BD FACS™ Lysing Solution (Cat. No. 349202) after staining.
      2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
      3. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
      4. An isotype control should be used at the same concentration as the antibody of interest.
      5. Please observe the following precautions: We recommend that special precautions be taken (such as wrapping vials, tubes, or racks in aluminum foil) to protect exposure of conjugated reagents, including cells stained with those reagents, to any room illumination. Absorption of visible light can significantly affect the emission spectra and quantum yield of tandem fluorochrome conjugates.
      6. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
      7. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
      8. Human donor specific background has been observed in relation to the presence of anti-polyethylene glycol (PEG) antibodies, developed as a result of certain vaccines containing PEG, including some COVID-19 vaccines. We recommend use of BD Horizon Brilliant™ Stain Buffer in your experiments to help mitigate potential background. For more information visit https://www.bdbiosciences.com/en-us/support/product-notices.
      9. Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
      10. Tandem fluorochromes contain both an energy donor and an energy acceptor. Although every effort is made to minimize the lot-to-lot variation in the efficiency of the fluorochrome energy transfer, differences in the residual emission from the donor may be observed. Additionally, multi-laser cytometers may directly excite both the donor and acceptor fluorochromes. Therefore, we recommend for every tandem conjugate, a matched individual single-stain control be acquired for generating a compensation or spectral unmixing matrix.
      11. Cy is a trademark of Global Life Sciences Solutions Germany GmbH or an affiliate doing business as Cytiva.
      12. For U.S. patents that may apply, see bd.com/patents.
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      571479 Rev. 1
      抗体详情
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      Streptavidin is a non-glycosylated protein that is prepared chromatographically from the bacterium Streptomyces avidinii. Streptavidin homotetramers have a particularly high, non-covalent binding affinity for biotin. When conjugated with fluorochromes, streptavidin has been widely used with biotin-conjugated antibodies and other biotinylated specific-binding molecules (eg, recombinant proteins and lectins) to stain cells and tissues for subsequent multiparameter analysis by flow cytometry, fluorescence microscopy and imaging. Likewise, when conjugated with an enzyme (eg, Horseradish Peroxidase or Alkaline Phosphatase) and coupled with a colorimetric or luminescent substrate development system, streptavidin has found widespread use along with biotinylated antibodies in a number of applications including Western blot, ELISA, ELISPOT, immunocytochemistry and immunohistochemistry.

      571479 Rev. 1
      格式详情
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      RY703
      The BD Horizon RealYellow™ 703 (RY703) Dye is part of the BD® family of yellow-green dyes. It is a tandem fluorochrome with an excitation maximum (Ex Max) at 557-nm and an emission maximum (Em Max) at 703-nm as measured using an antibody-dye conjugate. Driven by BD® innovation, RY703 can be used on both spectral and conventional cytometers and is designed to be excited by the Yellow-Green laser (561-nm) with minimal excitation by the 488-nm Blue laser. For conventional instruments equipped with a Yellow-Green laser (561-nm), RY703 can be used as an alternative to PE-Cy5.5 and we recommend using an optical filter centered near 700-nm (eg, a 695/40-nm bandpass filter).
      altImg
      RY703
      Yellow-Green 561 nm
      557 nm
      703 nm
      571479 Rev.1
      报价单和参考
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      View product citations for antibody "571479" on CiteAb

      研发参考 (2)

      1. Diamandis EP, Christopoulos TK. The biotin-(strept)avidin system: principles and applications in biotechnology. Clin Chem. 1991; 37(5):625-636. (Methodology). 查看参考
      2. Shapiro HM. Practical flow cytometry, 4th ed.. Hoboken, N.J.: Wiley-Liss; 2003:1-681.
      571479 Rev. 1

      Please refer to Support Documents for Quality Certificates

       

      Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

       

      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

      For Research Use Only. Not for use in diagnostic or therapeutic procedures.