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      RY703 Mouse Anti-Mouse CD66a (CEACAM1α)

      BD OptiBuild™ RY703 Mouse Anti-Mouse CD66a (CEACAM1α)

      克隆 CC1 (also known as MAb-CCl; mAb CC1)

      (RUO)
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      BD OptiBuild™
      CEACAM1[a]; Ceacam1; BGP-1; Bgp; MHV-R; MHVR
      Mouse (Tested in Development)
      Mouse SJL IgG1, κ
      BALB/c mouse intestinal brush border membrane proteins
      Flow cytometry (Qualified)
      0.2 mg/ml
      Aqueous buffered solution containing ≤0.09% sodium azide.
      RUO


      准备和存储

      Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to the dye under optimum conditions that minimize unconjugated dye and antibody.
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      推荐的实验流程

      BD® CompBeads can be used as surrogates to assess fluorescence spillover (compensation). When fluorochrome conjugated antibodies are bound to BD® CompBeads, they have spectral properties very similar to cells. However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls. It is strongly recommended that when using a reagent for the first time, users compare the spillover on cells and BD® CompBeads to ensure that BD® CompBeads are appropriate for your specific cellular application.

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      商品通知

      1. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
      2. Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
      3. For U.S. patents that may apply, see bd.com/patents.
      4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
      5. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
      6. The production process underwent stringent testing and validation to assure that it generates a high-quality conjugate with consistent performance and specific binding activity. However, verification testing has not been performed on all conjugate lots.
      7. When using high concentrations of antibody, background binding of this dye to erythroid fragments produced by ammonium chloride-based lysis, such as with BD Pharm Lyse™ Lysing Buffer (Cat. No. 555899), has been observed when the antibody conjugate was present during the lysis procedure. This may cause nonspecific staining of target cells, such as leukocytes, which have bound the resulting erythroid fragments. This background can be mitigated by any of the following: titrating the antibody conjugate to a lower concentration, fixing samples with formaldehyde, or removing erythrocytes before staining (eg, gradient centrifugation or pre-lysis with wash). This background has not been observed when cells were lysed with BD FACS™ Lysing Solution (Cat. No. 349202) after staining.
      8. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
      9. An isotype control should be used at the same concentration as the antibody of interest.
      10. Cy is a trademark of Global Life Sciences Solutions Germany GmbH or an affiliate doing business as Cytiva.
      11. Please observe the following precautions: We recommend that special precautions be taken (such as wrapping vials, tubes, or racks in aluminum foil) to protect exposure of conjugated reagents, including cells stained with those reagents, to any room illumination. Absorption of visible light can significantly affect the emission spectra and quantum yield of tandem fluorochrome conjugates.
      12. Tandem fluorochromes contain both an energy donor and an energy acceptor. Although every effort is made to minimize the lot-to-lot variation in the efficiency of the fluorochrome energy transfer, differences in the residual emission from the donor may be observed. Additionally, multi-laser cytometers may directly excite both the donor and acceptor fluorochromes. Therefore, we recommend for every tandem conjugate, a matched individual single-stain control be acquired for generating a compensation or spectral unmixing matrix.
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      771368 Rev. 1
      抗体详情
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      CC1

      The CC1 monoclonal antibody specifically recognizes carcinoembryonic antigen-related cell adhesion molecule 1a (CEACAM1a or CEACAM1[a]), an allotypic form of CEACAM1 which is also known as CD66a, Murine hepatitis virus receptor (MHV-R), or Biliary glycoprotein 1 (BGP-1). Four known isoforms of mouse CD66a arise from alternative splicing of RNA transcripts encoded by Ceacam1, a member of the carcinoembryonic antigen (CEA) family and Ig gene superfamily. These isoforms are type I transmembrane proteins that include a heavily glycosylated extracellular region with an N-terminal IgV-like domain and up to three IgC2-like domains followed by a transmembrane region and a cytoplasmic tail of relatively short (10 amino acids) or long (73 amino acids) length. The cytoplasmic tails enable interactions with other intracellular molecules to initiate or regulate cellular responses. The two CD66a isoforms with long cytoplasmic tails contain immunoreceptor tyrosine-based inhibitory motifs (ITIMs) that may enable CD66a to function as an immune checkpoint inhibitor. CD66a is expressed on a variety of cell types including certain epithelial cells, endothelial cells, B cells, activated T cells, NK cells, monocytes, dendritic cells (DC), and neutrophils. CD66a (CEACAM1a) is a multifunctional protein. Through their IgV-like domain, CD66a (CEACAM1a) molecules function as homophilic and heterophilic intercellular adhesion molecules. They can also function as MHV-Rs, angiogenic factors, regulators of cellular proliferation and differentiation, and tumor suppressors. Two distinct Ceacam1 alleles, (a and b), exist because of differences in their IgV-like domain gene sequences. Ceacam1a is found in most inbred mouse strains including BALB/c, C57BL/6, and C3H mice whereas Ceacam1b is found in SJL mice. CD66a (CEACAM1a) proteins are specifically bound by the CC1 antibody whereas CEACAM1b proteins are not. The CC1 antibody recognizes an epitope in the N-terminal domain of mouse CD66a (CEACAM1a) proteins.

      771368 Rev. 1
      格式详情
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      RY703
      The BD Horizon RealYellow™ 703 (RY703) Dye is part of the BD® family of yellow-green dyes. It is a tandem fluorochrome with an excitation maximum (Ex Max) at 557-nm and an emission maximum (Em Max) at 703-nm as measured using an antibody-dye conjugate. Driven by BD® innovation, RY703 can be used on both spectral and conventional cytometers and is designed to be excited by the Yellow-Green laser (561-nm) with minimal excitation by the 488-nm Blue laser. For conventional instruments equipped with a Yellow-Green laser (561-nm), RY703 can be used as an alternative to PE-Cy5.5 and we recommend using an optical filter centered near 700-nm (eg, a 695/40-nm bandpass filter).
      altImg
      RY703
      Yellow-Green 561 nm
      557 nm
      703 nm
      771368 Rev.1
      报价单和参考
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      View product citations for antibody "771368" on CiteAb

      研发参考 (15)

      1. Anczurowski M, Hirano N. Mechanisms of HLA-DP Antigen Processing and Presentation Revisited.. Trends Immunol. 2018; 39(12):960-964. (Biology). 查看参考
      2. Chen Z, Chen L, Baker K, et al. CEACAM1 dampens antitumor immunity by down-regulating NKG2D ligand expression on tumor cells.. J Exp Med. 2011; 208(13):2633-40. (Clone-specific: Flow cytometry). 查看参考
      3. Dveksler GS, Dieffenbach CW, Cardellichio CB, et al. J Virol. 1993; 67(1):1-8. (Clone-specific). 查看参考
      4. Dveksler GS, Pensiero MN, Cardellichio CB, et al. Cloning of the mouse hepatitis virus (MHV) receptor: expression in human and hamster cell lines confers susceptibility to MHV.. J Virol. 1991; 65(12):6881-91. (Clone-specific: Blocking). 查看参考
      5. Gray-Owen SD, Blumberg RS. CEACAM1: contact-dependent control of immunity.. Nat Rev Immunol. 2006; 6(6):433-46. (Biology). 查看参考
      6. Hemmila E, Turbide C, Olson M, Jothy S, Holmes KV, Beauchemin N. Ceacam1a-/- mice are completely resistant to infection by murine coronavirus mouse hepatitis virus A59.. J Virol. 2004; 78(18):10156-65. (Clone-specific). 查看参考
      7. Holmes KV, Dveksler G, Gagneten S, et al. Coronavirus receptor specificity.. Adv Exp Med Biol. 1993; 342:261-6. (Clone-specific: Immunoaffinity chromatography). 查看参考
      8. Khairnar V, Duhan V, Maney SK, et al. CEACAM1 induces B-cell survival and is essential for protective antiviral antibody production.. Nat Commun. 2015; 6:6217. (Clone-specific: Flow cytometry). 查看参考
      9. Lennon-Duménil AM, Barbouche MR, Vedrenne J, et al. Uncoordinated HLA-D gene expression in a RFXANK-defective patient with MHC class II deficiency.. J Immunol. 2001; 166(9):5681-7. (Clone-specific: Flow cytometry). 查看参考
      10. Nakajima A, Iijima H, Neurath MF, et al. Activation-induced expression of carcinoembryonic antigen-cell adhesion molecule 1 regulates mouse T lymphocyte function.. J Immunol. 2002; 168(3):1028-35. (Clone-specific: Flow cytometry). 查看参考
      11. Robbins PA, Evans EL, Ding AH, Warner NL, Brodsky FM. Monoclonal antibodies that distinguish between class II antigens (HLA-DP, DQ, and DR) in 14 haplotypes.. Hum Immunol. 1987; 18(4):301-13. (Clone-specific: Blocking, ELISA, Flow cytometry, Immunoprecipitation, Radioimmunoassay). 查看参考
      12. Royston I, Omary MB, Trowbridge IS. Monoclonal antibodies to a human T-cell antigen and Ia-like antigen in the characterization of lymphoid leukemia.. Transplant Proc. 1981; 13(1 Pt 2):761-6. (Immunogen: Fluorescence microscopy, Immunofluorescence, Immunoprecipitation, Radioimmunoassay). 查看参考
      13. Strobl H, Bello-Fernandez C, Riedl E, et al. flt3 ligand in cooperation with transforming growth factor-beta1 potentiates in vitro development of Langerhans-type dendritic cells and allows single-cell dendritic cell cluster formation under serum-free conditions.. Blood. 1997; 90(4):1425-34. (Clone-specific: Flow cytometry). 查看参考
      14. Williams RK, Jiang GS, Holmes KV. Receptor for mouse hepatitis virus is a member of the carcinoembryonic antigen family of glycoproteins.. Proc Natl Acad Sci U S A. 1991; 88(13):5533-6. (Clone-specific: Immunoaffinity chromatography, Radioimmunoassay). 查看参考
      15. Williams RK, Jiang GS, Snyder SW, Frana MF, Holmes KV. Purification of the 110-kilodalton glycoprotein receptor for mouse hepatitis virus (MHV)-A59 from mouse liver and identification of a nonfunctional, homologous protein in MHV-resistant SJL/J mice.. J Virol. 1990; 64(8):3817-23. (Immunogen: Blocking, ELISA, Immunoaffinity chromatography, Inhibition, Radioimmunoassay). 查看参考
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      771368 Rev. 1

       

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      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

      For Research Use Only. Not for use in diagnostic or therapeutic procedures.