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      RY610 Rat Anti-Human IL-13
      RY610 Rat Anti-Human IL-13
      Expression of IL-13 by stimulated Human peripheral blood lymphocytes. Human peripheral blood mononuclear cells (PBMC) were stimulated for 6 h with Phorbol 12-Myristate 13-Acetate (PMA; Sigma, Cat. No. P-8139; 50 ng/ml) and Ionomycin (Sigma, Cat. No. I-0634; 1 μg/ml) in the presence of GolgiStop™ Protein Transport Inhibitor (Cat. No. 554724). The cells were fixed with BD Cytofix™ Fixation Buffer (Cat. No. 554655) and then washed with and stained in BD Perm/Wash™ Buffer (Cat. No. 554723) with FITC Mouse Anti-Human CD4 antibody (Cat. No. 566911) and with either BD Horizon™ RY610 Rat IgG1, κ Isotype Control (Cat. No. 571676; Left Plot) or BD Horizon™ RY610 Rat Anti-Human IL-13 antibody (Cat. No. 571260/571320; Right Plot) using BD Biosciences Immunofluorescent Staining of Intracellular Cytokines for Flow Cytometric Analysis protocol. The bivariate pseudocolor density plot showing the correlated expression of IL-13 (or Ig Isotype control staining) versus CD4 was derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometry and data analysis were performed using a BD Symphony A5™ Cell Analyzer System and FlowJo™ Software.
      RY610 Rat Anti-Human IL-13
      Expression of IL-13 by stimulated Human peripheral blood lymphocytes. Human peripheral blood mononuclear cells (PBMC) were stimulated for 6 h with Phorbol 12-Myristate 13-Acetate (PMA; Sigma, Cat. No. P-8139; 50 ng/ml) and Ionomycin (Sigma, Cat. No. I-0634; 1 μg/ml) in the presence of GolgiStop™ Protein Transport Inhibitor (Cat. No. 554724). The cells were fixed with BD Cytofix™ Fixation Buffer (Cat. No. 554655) and then washed with and stained in BD Perm/Wash™ Buffer (Cat. No. 554723) with FITC Mouse Anti-Human CD4 antibody (Cat. No. 566911) and with either BD Horizon™ RY610 Rat IgG1, κ Isotype Control (Cat. No. 571676; Left Plot) or BD Horizon™ RY610 Rat Anti-Human IL-13 antibody (Cat. No. 571260/571320; Right Plot) using BD Biosciences Immunofluorescent Staining of Intracellular Cytokines for Flow Cytometric Analysis protocol. The bivariate pseudocolor density plot showing the correlated expression of IL-13 (or Ig Isotype control staining) versus CD4 was derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometry and data analysis were performed using a BD Symphony A5™ Cell Analyzer System and FlowJo™ Software.
      商品详情
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      BD Horizon™
      Interleukin-13; NC30; ALRH; BHR1; P600
      Human (QC Testing)
      Rat IgG1
      Human Recombinant IL-13
      Intracellular staining (flow cytometry) (Routinely Tested)
      5 µl/test
      3596
      Aqueous buffered solution containing ≤0.09% sodium azide.
      RUO


      准备和存储

      The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to the dye under optimum conditions and unreacted dye was removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
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      推荐的实验流程

      BD® CompBeads can be used as surrogates to assess fluorescence spillover (compensation). When fluorochrome conjugated antibodies are bound to BD® CompBeads, they have spectral properties very similar to cells. However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls. It is strongly recommended that when using a reagent for the first time, users compare the spillover on cells and BD® CompBeads to ensure that BD® CompBeads are appropriate for your specific cellular application.

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      商品通知

      1. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
      2. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
      3. This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
      4. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
      5. An isotype control should be used at the same concentration as the antibody of interest.
      6. Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
      7. Human donor specific background has been observed in relation to the presence of anti-polyethylene glycol (PEG) antibodies, developed as a result of certain vaccines containing PEG, including some COVID-19 vaccines. We recommend use of BD Horizon Brilliant™ Stain Buffer in your experiments to help mitigate potential background. For more information visit https://www.bdbiosciences.com/en-us/support/product-notices.
      8. Please observe the following precautions: We recommend that special precautions be taken (such as wrapping vials, tubes, or racks in aluminum foil) to protect exposure of conjugated reagents, including cells stained with those reagents, to any room illumination. Absorption of visible light can significantly affect the emission spectra and quantum yield of tandem fluorochrome conjugates.
      9. CF™ is a trademark of Biotium, Inc.
      10. For U.S. patents that may apply, see bd.com/patents.
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      571320 Rev. 1
      抗体详情
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      JES10-5A2

      The JES10-5A2 monoclonal antibody specifically binds to human interleukin-13, IL-13. IL-13 is produced by activated T cells, mast cells and NK cells. IL-13 regulates IgE production by B cells and can suppress the cytotoxic activity of macrophages and their production of inflammatory mediators. The immunogen used to produce the JES10-5A2 hybridoma was COS-expressed recombinant human IL-13. This is a neutralizing antibody.

      571320 Rev. 1
      格式详情
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      RY610
      The BD Horizon RealYellow™ 610 (RY610) Dye is part of the BD® family of yellow-green dyes. It is a tandem fluorochrome with an excitation maximum (Ex Max) at 557-nm and an emission maximum (Em Max) at 610-nm as measured using an antibody-dye conjugate. Driven by BD® innovation, RY610 can be used on both spectral and conventional cytometers and is designed to be excited by the Yellow-Green laser (561-nm) with minimal excitation by the 488-nm blue laser. For conventional instruments equipped with a Yellow-Green laser (561-nm), RY610 can be used as an alternative to PE-CF594 and we recommend using an optical filter centered near 610-nm (eg, a 610/20-nm bandpass filter). For spectral instruments equipped with a Yellow-Green laser (561-nm), it can be used in conjunction with PE-CF594.
      altImg
      RY610
      Yellow-Green 561 nm
      557 nm
      610 nm
      571320 Rev.1
      报价单和参考
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      View product citations for antibody "571320" on CiteAb

      研发参考 (7)

      1. Abrams J. Immunoenzymetric assay of mouse and human cytokines using NIP-labeled anti-cytokine antibodies. Curr Protoc Immunol. 2001; 1:6.20-6.21. (Clone-specific). 查看参考
      2. Andersson J, Abrams J, Bjork L, et al. Concomitant in vivo production of 19 different cytokines in human tonsils. Immunology. 1994; 83(1):16-24. (Clone-specific: Immunohistochemistry). 查看参考
      3. Andersson U, Andersson J. Immunolabeling of cytokine-producing cells in tissues and in suspension. In: Fradelizie D, Emelie D, ed. Cytokine Producing Cells. Paris: Inserm; 1994:32-49.
      4. Litton M, Andersson J, Bjork L, Fehniger T, Ulfgren AK, Andersson U. Cytoplasmic cytokine staining in individual cells. In: Debets and Savelkoul, ed. Human Cytokine Protocols. Humana Press; 1996.
      5. McKenzie ANJ, Matthews DJ. IL-13. In: Oppenheim JJ, Feldmann M, Durum SK, Hirano T, Vilcek J, Nicola NA, ed. Cytokine Reference : A compendium of cytokines and other mediators of host defense. San Diego: Academic Press; 2001:203-211.
      6. Prussin C, Metcalfe DD. Detection of intracytoplasmic cytokine using flow cytometry and directly conjugated anti-cytokine antibodies. J Immunol Methods. 1995; 188(1):117-128. (Methodology: Flow cytometry). 查看参考
      7. Schaerli P, Willimann K, Lang AB, Lipp M, Loetscher P, Moser B. CXC chemokine receptor 5 expression defines follicular homing T cells with B cell helper function. J Exp Med. 2000; 192(11):1553-1562. (Clone-specific: Flow cytometry). 查看参考
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      571320 Rev. 1

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