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      RY610 Mouse Anti-Human Fetal Hemoglobin
      RY610 Mouse Anti-Human Fetal Hemoglobin
      Flow cytometric analysis of Fetal Hemoglobin expression in fetal cord blood erythrocytes.  Fetal cord blood erythrocytes were fixed (10 min; room temperature) with 0.05% cold glutaraldehyde and then permeabilized with 0.1% Triton X-100 (10 min, RT). The cells were then stained with either BD Horizon™ RY610 Mouse IgG1, κ Isotype Control (Cat. No. 571151; dashed line histogram) or BD Horizon™ RY610 Mouse Anti-Human Fetal Hemoglobin antibody (Cat. No. 571208/571212; solid line histogram). The fluorescence histogram showing Fetal Hemoglobin expression (or Ig Isotype control staining) was derived from gated events with the forward and side light-scatter characteristics of intact erythrocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa™ X-20 Cell Analyzer System and FlowJo™ Software.
      RY610 Mouse Anti-Human Fetal Hemoglobin
      Flow cytometric analysis of Fetal Hemoglobin expression in fetal cord blood erythrocytes.  Fetal cord blood erythrocytes were fixed (10 min; room temperature) with 0.05% cold glutaraldehyde and then permeabilized with 0.1% Triton X-100 (10 min, RT). The cells were then stained with either BD Horizon™ RY610 Mouse IgG1, κ Isotype Control (Cat. No. 571151; dashed line histogram) or BD Horizon™ RY610 Mouse Anti-Human Fetal Hemoglobin antibody (Cat. No. 571208/571212; solid line histogram). The fluorescence histogram showing Fetal Hemoglobin expression (or Ig Isotype control staining) was derived from gated events with the forward and side light-scatter characteristics of intact erythrocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa™ X-20 Cell Analyzer System and FlowJo™ Software.
      商品详情
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      BD Horizon™
      Human (QC Testing)
      Mouse IgG1, κ
      Intracellular staining (flow cytometry) (Routinely Tested)
      5 µl/test
      Aqueous buffered solution containing ≤0.09% sodium azide.
      RUO


      准备和存储

      The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to the dye under optimum conditions and unreacted dye was removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
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      推荐的实验流程

      BD® CompBeads can be used as surrogates to assess fluorescence spillover (compensation). When fluorochrome conjugated antibodies are bound to BD® CompBeads, they have spectral properties very similar to cells. However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls. It is strongly recommended that when using a reagent for the first time, users compare the spillover on cells and BD® CompBeads to ensure that BD® CompBeads are appropriate for your specific cellular application.

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      商品通知

      1. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
      2. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
      3. This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
      4. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
      5. An isotype control should be used at the same concentration as the antibody of interest.
      6. Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
      7. Human donor specific background has been observed in relation to the presence of anti-polyethylene glycol (PEG) antibodies, developed as a result of certain vaccines containing PEG, including some COVID-19 vaccines. We recommend use of BD Horizon Brilliant™ Stain Buffer in your experiments to help mitigate potential background. For more information visit https://www.bdbiosciences.com/en-us/support/product-notices.
      8. Please observe the following precautions: We recommend that special precautions be taken (such as wrapping vials, tubes, or racks in aluminum foil) to protect exposure of conjugated reagents, including cells stained with those reagents, to any room illumination. Absorption of visible light can significantly affect the emission spectra and quantum yield of tandem fluorochrome conjugates.
      9. CF™ is a trademark of Biotium, Inc.
      10. For U.S. patents that may apply, see bd.com/patents.
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      571212 Rev. 1
      抗体详情
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      2D12

      Reacts with fetal hemoglobin (HbF), a form of hemoglobin present during fetal development. HbF resembles adult hemoglobin (HbA) in possessing two alpha chains but differs in possessing two gamma instead of two beta chains. In normal adults, synthesis of HbF does persist at very low levels (<1% of total Hb) and is restricted to a small population of erythrocytes called F cells. Hemoglobin F-expressing erythrocytes are normally seen during pregnancy. An increase in the expression of fetal hemoglobin in adult peripheral red blood cells is a common feature in the genetic disorders of hemoglobin, sickle-cell disease (SCD) and beta thalassemia.

      571212 Rev. 1
      格式详情
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      RY610
      The BD Horizon RealYellow™ 610 (RY610) Dye is part of the BD® family of yellow-green dyes. It is a tandem fluorochrome with an excitation maximum (Ex Max) at 557-nm and an emission maximum (Em Max) at 610-nm as measured using an antibody-dye conjugate. Driven by BD® innovation, RY610 can be used on both spectral and conventional cytometers and is designed to be excited by the Yellow-Green laser (561-nm) with minimal excitation by the 488-nm blue laser. For conventional instruments equipped with a Yellow-Green laser (561-nm), RY610 can be used as an alternative to PE-CF594 and we recommend using an optical filter centered near 610-nm (eg, a 610/20-nm bandpass filter). For spectral instruments equipped with a Yellow-Green laser (561-nm), it can be used in conjunction with PE-CF594.
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      RY610
      Yellow-Green 561 nm
      557 nm
      610 nm
      571212 Rev.1
      报价单和参考
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      View product citations for antibody "571212" on CiteAb

      研发参考 (3)

      1. Campbell TA, Ware RE, Mason M. Detection of hemoglobin variants in erythrocytes by flow cytometry. Cytometry. 1999; 35(3):242-248. (Biology). 查看参考
      2. Horiuchi K, Osterhout ML, Kamma H, Bekoe NA, Hirokawa KJ. Estimation of fetal hemoglobin levels in individual red cells via fluorescence image cytometry. Cytometry. 1995; 20(3):261-267. (Biology). 查看参考
      3. Thorpe SJ, Thein SL, Sampietro M, Craig JE, Mahon B, Huehns ER. Immunochemical estimation of haemoglobin types in red blood cells by FACS analysis. Br J Haematol. 1994; 87(1):125-132. (Biology). 查看参考
      571212 Rev. 1

      Please refer to Support Documents for Quality Certificates

       

      Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

       

      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

      For Research Use Only. Not for use in diagnostic or therapeutic procedures.