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      RB780 Mouse Anti-Human CD58 (LFA-3)
      商品详情
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      BD OptiBuild™
      LFA-3; LFA3; Lymphocyte function-associated antigen 3; Ag3
      Human (Tested in Development)
      Mouse IgG2a, κ
      Lc-LFA-3 mouse L-cell transfectant
      Flow cytometry (Qualified)
      0.2 mg/ml
      V πNK15
      Aqueous buffered solution containing ≤0.09% sodium azide.
      RUO


      准备和存储

      Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to the dye under optimum conditions that minimize unconjugated dye and antibody.
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      推荐的实验流程

      BD® CompBeads can be used as surrogates to assess fluorescence spillover (compensation). When fluorochrome conjugated antibodies are bound to BD® CompBeads, they have spectral properties very similar to cells. However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls. It is strongly recommended that when using a reagent for the first time, users compare the spillover on cells and BD® CompBeads to ensure that BD® CompBeads are appropriate for your specific cellular application.

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      商品通知

      1. When using high concentrations of antibody, background binding of this dye to erythroid fragments produced by ammonium chloride-based lysis, such as with BD Pharm Lyse™ Lysing Buffer (Cat. No. 555899), has been observed when the antibody conjugate was present during the lysis procedure. This may cause nonspecific staining of target cells, such as leukocytes, which have bound the resulting erythroid fragments. This background can be mitigated by any of the following: titrating the antibody conjugate to a lower concentration, fixing samples with formaldehyde, or removing erythrocytes before staining (eg, gradient centrifugation or pre-lysis with wash). This background has not been observed when cells were lysed with BD FACS™ Lysing Solution (Cat. No. 349202) after staining.
      2. Researchers should determine the optimal concentration of this reagent for their individual applications.
      3. The production process underwent stringent testing and validation to assure that it generates a high-quality conjugate with consistent performance and specific binding activity. However, verification testing has not been performed on all conjugate lots.
      4. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
      5. Please observe the following precautions: Absorption of visible light can significantly alter the energy transfer occurring in any tandem fluorochrome conjugate; therefore, we recommend that special precautions be taken (such as wrapping vials, tubes, or racks in aluminum foil) to prevent exposure of conjugated reagents, including cells stained with those reagents, to room illumination.
      6. An isotype control should be used at the same concentration as the antibody of interest.
      7. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
      8. Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
      9. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
      10. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
      11. Cy is a trademark of Global Life Sciences Solutions Germany GmbH or an affiliate doing business as Cytiva.
      12. Human donor specific background has been observed in relation to the presence of anti-polyethylene glycol (PEG) antibodies, developed as a result of certain vaccines containing PEG, including some COVID-19 vaccines. We recommend use of BD Horizon Brilliant™ Stain Buffer in your experiments to help mitigate potential background. For more information visit https://www.bdbiosciences.com/en-us/support/product-notices.
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      755543 Rev. 1
      抗体详情
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      L306.4

      The L306.4  monoclonal antibody specifically recognizes CD58 which is also known as Lymphocyte function-associated antigen-3 (LFA-3) or Ag3. CD58 is a ~40 to 65 kDa cell-surface glycoprotein that belongs to the immunoglobulin superfamily. The CD58 antigen mediates cellular adhesion and participates in signal transduction when it binds to its ligand, the CD2 antigen. Cellular interactions regulated by the CD58/CD2 antigens are involved in the antigen-independent adhesion pathway and cytotoxic T lymphocyte (CTL) activity. The CD58 antigen has two isoforms. One isoform is anchored in the cell membrane by a glycophosphatidyl inositol tail, while the other is a type I transmembrane glycoprotein which has a transmembrane hydrophobic segment and a cytoplasmic segment composed of 12 amino acids. The CD58 antigen is widely distributed on cells of both hematopoietic and nonhematopoietic origin. The CD58 antigen is expressed on approximately 40% to 60% of peripheral blood lymphocytes, including CTL. It is also expressed on monocytes, granulocytes, B lymphoblastoid cell lines (such as JY and Daudi), platelets, vascular endothelium and smooth muscle, fibroblasts, and approximately 40% of bone marrow cells.

      755543 Rev. 1
      格式详情
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      RB780
      The BD Horizon RealBlue™ 780 (RB780) Dye is part of the BD family of blue dyes. It is a tandem fluorochrome with an excitation maximum (Ex Max) at 498-nm and an emission maximum (Em Max) at 781-nm. Driven by BD innovation, RB780 can be used on both spectral and conventional cytometers and is designed to be excited by the Blue laser (488-nm) with minimal excitation by the 561-nm Yellow-Green laser. For conventional instruments equipped with a Blue laser (488-nm), RB780 can be used as an alternative to PE-Cy7 and we recommend using an optical filter centered near 780-nm (eg, a 780/60-nm bandpass filter). For spectral instruments equipped with a Blue laser (488-nm), it can be used in conjunction with PE-Cy7. RB780 is on average brighter than PE-Cy7 and has minimal spillover into Yellow-Green detectors.
      altImg
      RB780
      Blue 488 nm
      498 nm
      781 nm
      755543 Rev.1
      报价单和参考
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      View product citations for antibody "755543" on CiteAb

      研发参考 (12)

      1. Rincón J, Patarroyo M. Effect of antibodies from the T-cell (‘CD2 only’) and the NK/non-lineage (new panel only) sections on adhesion of Jurkat (T) cells to human erythrocytes. In: Knapp W. W. Knapp .. et al., ed. Leucocyte typing IV : white cell differentiation antigens. Oxford New York: Oxford University Press; 1989:718-720.
      2. Carpén O, Dustin ML, Springer TA, Swafford JA, Beckett LA, Caulfield JP. Motility and ultrastructure of large granular lymphocytes on lipid bilayers reconstituted with adhesion receptors LFA-1, ICAM-1, and two isoforms of LFA-3.. J Cell Biol. 1991; 115(3):861-71. (Biology). 查看参考
      3. Dengler TJ, Hoffmann JC, Knolle P, et al. Structural and functional epitopes of the human adhesion receptor CD58 (LFA-3). Eur J Immunol. 1992; 22(11):2809-2817. (Biology). 查看参考
      4. Griffin H, Rowe M, Murray R, Brooks J, Rickinson A. Restoration of the LFA-3 adhesion pathway in Burkitt's lymphoma cells using an LFA-3 recombinant vaccinia virus: consequences for T cell recognition.. Eur J Immunol. 1992; 22(7):1741-8. (Biology). 查看参考
      5. Krensky AM, Robbins E, Springer TA, Burakoff SJ. LFA-1, LFA-2, and LFA-3 antigens are involved in CTL-target conjugation.. J Immunol. 1984; 132(5):2180-2. (Biology). 查看参考
      6. Krensky AM, Sanchez-Madrid F, Robbins E, Nagy JA, Springer TA, Burakoff SJ. The functional significance, distribution, and structure of LFA-1, LFA-2, and LFA-3: cell surface antigens associated with CTL-target interactions.. J Immunol. 1983; 131(2):611-6. (Biology). 查看参考
      7. Scheeren RA, Koopman G, Van der Baan S, Meijer CJ, Pals ST. Adhesion receptors involved in clustering of blood dendritic cells and T lymphocytes.. Eur J Immunol. 1991; 21(5):1101-5. (Biology). 查看参考
      8. Selvaraj P, Plunkett ML, Dustin M, Sanders ME, Shaw S, Springer TA. The T lymphocyte glycoprotein CD2 binds the cell surface ligand LFA-3.. Nature. 326(6111):400-3. (Biology). 查看参考
      9. Shaw S, Johnson JP. Cluster report: CD58. In: Knapp W. W. Knapp .. et al., ed. Leucocyte typing IV : white cell differentiation antigens. Oxford New York: Oxford University Press; 1989:714-716.
      10. Shaw S, Luce GE, Quinones R, Gress RE, Springer TA, Sanders ME. Two antigen-independent adhesion pathways used by human cytotoxic T-cell clones.. Nature. 323(6085):262-4. (Biology). 查看参考
      11. Smith ME, Thomas JA. Cellular expression of lymphocyte function associated antigens and the intercellular adhesion molecule-1 in normal tissue.. J Clin Pathol. 1990; 43(11):893-900. (Clone-specific). 查看参考
      12. Springer TA. Adhesion receptors of the immune system. Nature. 1990; 346(6283):425-434. (Biology). 查看参考
      查看所有文件 (12) 查看更少内容
      755543 Rev. 1

      Please refer to Support Documents for Quality Certificates


      Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

      For Research Use Only. Not for use in diagnostic or therapeutic procedures.