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RB705 Mouse Anti-Human CD142
商品详情
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BD OptiBuild™
Tissue factor; TF; TFA; Coagulation factor III; F3; Thromboplastin
Human (Tested in Development)
Mouse IgG1, κ
Human Tissue Factor Protein
Flow cytometry (Qualified)
0.2 mg/ml
VI E016, E137
2152
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO


准备和存储

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to the dye under optimum conditions that minimize unconjugated dye and antibody.

推荐的实验流程

BD® CompBeads can be used as surrogates to assess fluorescence spillover (compensation). When fluorochrome conjugated antibodies are bound to BD® CompBeads, they have spectral properties very similar to cells. However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls. It is strongly recommended that when using a reagent for the first time, users compare the spillover on cells and BD® CompBeads to ensure that BD® CompBeads are appropriate for your specific cellular application.

商品通知

  1. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  2. Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
  3. For U.S. patents that may apply, see bd.com/patents.
  4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  5. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  6. The production process underwent stringent testing and validation to assure that it generates a high-quality conjugate with consistent performance and specific binding activity. However, verification testing has not been performed on all conjugate lots.
  7. Human donor specific background has been observed in relation to the presence of anti-polyethylene glycol (PEG) antibodies, developed as a result of certain vaccines containing PEG, including some COVID-19 vaccines. We recommend use of BD Horizon Brilliant™ Stain Buffer in your experiments to help mitigate potential background. For more information visit https://www.bdbiosciences.com/en-us/support/product-notices.
  8. When using high concentrations of antibody, background binding of this dye to erythroid fragments produced by ammonium chloride-based lysis, such as with BD Pharm Lyse™ Lysing Buffer (Cat. No. 555899), has been observed when the antibody conjugate was present during the lysis procedure. This may cause nonspecific staining of target cells, such as leukocytes, which have bound the resulting erythroid fragments. This background can be mitigated by any of the following: titrating the antibody conjugate to a lower concentration, fixing samples with formaldehyde, or removing erythrocytes before staining (eg, gradient centrifugation or pre-lysis with wash). This background has not been observed when cells were lysed with BD FACS™ Lysing Solution (Cat. No. 349202) after staining.
  9. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
  10. An isotype control should be used at the same concentration as the antibody of interest.
  11. Cy is a trademark of Global Life Sciences Solutions Germany GmbH or an affiliate doing business as Cytiva.
  12. Please observe the following precautions: We recommend that special precautions be taken (such as wrapping vials, tubes, or racks in aluminum foil) to protect exposure of conjugated reagents, including cells stained with those reagents, to any room illumination. Absorption of visible light can significantly affect the emission spectra and quantum yield of tandem fluorochrome conjugates.
757407 Rev. 1
抗体详情
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HTF-1

The HTF-1 monoclonal antibody specifically binds to CD142. CD142 is a 45-47 kDa, single chain, type I transmembrane glycoprotein also known as Tissue Factor (TF). CD142 has been referred in the literature as coagulation Factor III or thromboplastin and it is expressed on activated endothelial cells and lipopolysaccharide (LPS)-stimulated monocytes/macrophages. TF associates with factor VIIa to form a complex and acts as an enzyme that initiates the blood coagulation cascade. It is known as the major initiator of clotting in normal hemostasis. CD142 can be induced by various inflammatory mediators in monocytes and vascular endothelial cells. This antibody is useful in inflammation, thrombosis and hemostasis research.

757407 Rev. 1
格式详情
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RB705
The BD Horizon RealBlue™ 705 (RB705) Dye is part of the BD® family of blue dyes. It is a tandem fluorochrome with an excitation maximum (Ex Max) at 498-nm and an emission maximum (Em Max) at 707-nm as measured using an antibody-dye conjugate. Driven by BD® innovation, RB705 can be used on both spectral and conventional cytometers and is designed to be excited by the Blue laser (488-nm) with minimal excitation by the 561-nm Yellow-Green laser. For conventional instruments equipped with a Blue laser (488-nm), RB705 can be used as an alternative to PerCP-Cy5.5 or BB700 and we recommend using an optical filter centered near 710-nm (e.g., a 695/40 or 710/50-nm bandpass filter). For spectral instruments equipped with a Blue laser (488-nm), it can be used in conjunction with PerCP-Cy5.5. RB705 is on average brighter than PerCP-Cy5.5 and BB700, and has minimal spillover into Yellow-Green detectors.
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RB705
Blue 488 nm
498 nm
707 nm
757407 Rev.1
报价单和参考
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View product citations for antibody "757407" on CiteAb

研发参考 (5)

  1. Carson SD, Perry GA, Pirruccello SJ. Fibroblast tissue factor: calcium and ionophore induce shape changes, release of membrane vesicles, and redistribution of tissue factor antigen in addition to increased procoagulant activity. Blood. 1994; 84(2):526-534. (Clone-specific: Fluorescence microscopy, Immunofluorescence). 查看参考
  2. Carson SD, Ross SE, Bach R, Guha A. An inhibitory monoclonal antibody against human tissue factor.. Blood. 1987; 70(2):490-3. (Immunogen: Dot Blot, ELISA, Flow cytometry, Inhibition, Western blot). 查看参考
  3. McComb RD, Miller KA, Carson SD. Tissue factor antigen in senile plaques of Alzheimer's disease. Am J Pathol. 1991; 139(3):491-494. (Clone-specific: Immunohistochemistry). 查看参考
  4. Morrissey JH, Agis H, Albrecht S, et al. CD142 (tissue factor) Workshop Panel report. In: Mason D. David Mason .. et al., ed. Leucocyte typing VII : white cell differentiation antigens : proceedings of the Seventh International Workshop and Conference held in Harrogate, United Kingdom. Oxford: Oxford University Press; 2002:742-746.
  5. Whittle SM, Yoder SC, Carson SD. Human placental tissue factor: protease susceptibility of extracellular and cytoplasmic domains. Thromb Res. 1995; 79(5-6):451-459. (Clone-specific: Western blot). 查看参考
查看所有文件 (5) 查看更少内容
757407 Rev. 1

 

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Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.