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      RB670 Mouse Anti-Human CD203c
      RB670 Mouse Anti-Human CD203c
      Multiparameter flow cytometric analysis using BD OptiBuild™ RB670 Mouse Anti-Human CD203c antibody (Cat. No. 770472) on human peripheral blood, with corresponding IgG Isotype Control (Cat. No. 571784; Left Plot).  Samples were acquired on the BD FACSymphony™ A5 SE Cell Analyzer.
      RB670 Mouse Anti-Human CD203c
      Multiparameter flow cytometric analysis using BD OptiBuild™ RB670 Mouse Anti-Human CD203c antibody (Cat. No. 770472) on human peripheral blood, with corresponding IgG Isotype Control (Cat. No. 571784; Left Plot).  Samples were acquired on the BD FACSymphony™ A5 SE Cell Analyzer.
      商品详情
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      BD OptiBuild™
      ENPP3; E-NPP 3; NPP3; PDNP3; PD-Ibeta; PD-1β; B10; NPPase; gp130RB13-6
      Human (Tested in Development)
      Mouse BALB/c IgG1, κ
      Human CD203c Transfected Cell Line
      Flow cytometry (Qualified)
      0.2 mg/ml
      5169
      Aqueous buffered solution containing ≤0.09% sodium azide.
      RUO


      准备和存储

      The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to the dye under optimum conditions that minimize unconjugated dye and antibody. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
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      推荐的实验流程

      BD® CompBeads can be used as surrogates to assess fluorescence spillover (compensation).  When fluorochrome conjugated antibodies are bound to BD® CompBeads, they have spectral properties very similar to cells.  However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls.  It is strongly recommended that when using a reagent for the first time, users compare the spillover on cells and BD® CompBeads to ensure that BD® CompBeads are appropriate for your specific cellular application.

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      商品通知

      1. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
      2. Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
      3. For U.S. patents that may apply, see bd.com/patents.
      4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
      5. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
      6. The production process underwent stringent testing and validation to assure that it generates a high-quality conjugate with consistent performance and specific binding activity. However, verification testing has not been performed on all conjugate lots.
      7. When using high concentrations of antibody, background binding of this dye to erythroid fragments produced by ammonium chloride-based lysis, such as with BD Pharm Lyse™ Lysing Buffer (Cat. No. 555899), has been observed when the antibody conjugate was present during the lysis procedure. This may cause nonspecific staining of target cells, such as leukocytes, which have bound the resulting erythroid fragments. This background can be mitigated by any of the following: titrating the antibody conjugate to a lower concentration, fixing samples with formaldehyde, or removing erythrocytes before staining (eg, gradient centrifugation or pre-lysis with wash). This background has not been observed when cells were lysed with BD FACS™ Lysing Solution (Cat. No. 349202) after staining.
      8. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
      9. An isotype control should be used at the same concentration as the antibody of interest.
      10. Please observe the following precautions: We recommend that special precautions be taken (such as wrapping vials, tubes, or racks in aluminum foil) to protect exposure of conjugated reagents, including cells stained with those reagents, to any room illumination. Absorption of visible light can significantly affect the emission spectra and quantum yield of tandem fluorochrome conjugates.
      11. Tandem fluorochromes contain both an energy donor and an energy acceptor. Although every effort is made to minimize the lot-to-lot variation in the efficiency of the fluorochrome energy transfer, differences in the residual emission from the donor may be observed. Additionally, multi-laser cytometers may directly excite both the donor and acceptor fluorochromes. Therefore, we recommend for every tandem conjugate, a matched individual single-stain control be acquired for generating a compensation or spectral unmixing matrix.
      12. Cy is a trademark of Global Life Sciences Solutions Germany GmbH or an affiliate doing business as Cytiva.
      13. Human donor specific background has been observed in relation to the presence of anti-polyethylene glycol (PEG) antibodies, developed as a result of certain vaccines containing PEG, including some COVID-19 vaccines. We recommend use of BD Horizon Brilliant™ Stain Buffer in your experiments to help mitigate potential background. For more information visit https://www.bdbiosciences.com/en-us/support/product-notices.
      14. Clones without a listed workshop have not been investigated in an HLDA workshop to receive a CD nomenclature. We use “CD” provisionally when our internal testing indicates that this clone binds to the same CD antigen as workshopped clones.
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      770472 Rev. 1
      抗体详情
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      NP4D6

      The NP4D6 monoclonal antibody specifically binds to CD203c. CD203c is a type II transmembrane glycoprotein that is a member of the E-NNP family of ectoenzymes. CD203c is also known as ectonucleotide pyrophosphatase/phosphodiesterase 3 (E-NPP3, ENPP3) due to its capacity to hydrolyze phosphodiester and phosphosulfate bonds in a variety of molecules including deoxynucleotides, nucleoside phosphates, and nicotinamide adenine dinucleotide. CD203c is otherwise known as Phosphodiesterase-1β (PD-1β, PD-I beta), B10, or gp130RB13-6. CD203c is expressed by basophils and mast cells. Basophils increase CD203c expression following activation with allergens or antibodies that crosslink cytophilic IgE. Thus, CD203c serves as a useful flow cytometric marker for basophil activation and the detection and analysis of type 1 allergic responses. IgE-receptor cross-linking results in CD203c upregulation and overexpression on neoplastic mast cells in cases of systemic mastocytosis.

      770472 Rev. 1
      格式详情
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      RB670
      The BD Horizon RealBlue™ 670 (RB670) Dye is part of the BD® family of blue dyes. It is a tandem fluorochrome with an excitation maximum (Ex Max) at 492 nm and an emission maximum (Em Max) at 670 nm as measured using an antibody-dye conjugate. Driven by BD® innovation, RB670 can be used on both spectral and conventional cytometers and is designed to be primarily excited by the Blue laser (488-nm). For conventional instruments equipped with only a Blue laser (488-nm), RB670 can be used as an alternative to PE-Cy5 and we recommend using an optical filter centered near 670-nm (eg, a 670/30-nm bandpass filter). For conventional and spectral instruments equipped with both a Blue (488-nm) and Yellow-Green (561-nm) laser and appropriate detectors, it can be used in conjunction with PE-Cy5.
      altImg
      RB670
      Blue 488 nm
      492 nm
      670 nm
      770472 Rev.1
      报价单和参考
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      View product citations for antibody "770472" on CiteAb

      研发参考 (8)

      1. Binder M, Fierlbeck G, King T, Valent P, Buhring HJ. Individual hymenoptera venom compounds induce upregulation of the basophil activation marker ectonucleotide pyrophosphatase/phosphodiesterase 3 (CD203c) in sensitized patients. Int Arch Allergy Immunol. 2002; 129(2):160-168. (Clone-specific: Flow cytometry). 查看参考
      2. Buhring HJ, Streble A, Valent P. The basophil-specific ectoenzyme E-NPP3 (CD203c) as a marker for cell activation and allergy diagnosis. Int Arch Allergy Immunol. 2004; 133(4):317-329. (Clone-specific: Flow cytometry). 查看参考
      3. Fureder W, Schernthaner GH, Ghannadan M, et al. Quantitative, phenotypic, and functional evaluation of basophils in myelodysplastic syndromes. Eur J Clin Invest. 2001; 31(10):894-901. (Biology). 查看参考
      4. Ghannadan M, Hauswirth AW, Schernthaner GH, et al. Detection of novel CD antigens on the surface of human mast cells and basophils. Int Arch Allergy Immunol. 2002; 127(4):299-307. (Clone-specific: Immunofluorescence). 查看参考
      5. Hauswirth AW, Natter S, Ghannadan M, et al. Recombinant allergens promote expression of CD203c on basophils in sensitized individuals. J Allergy Clin Immunol. 2002; 110(1):102-109. (Clone-specific: Flow cytometry). 查看参考
      6. Hauswirth AW, Sonneck K, Florian S, et al. Interleukin-3 promotes the expression of E-NPP3/CD203C on human blood basophils in healthy subjects and in patients with birch pollen allergy. Int J Immunopathol Pharmacol. 2007; 20(2):267-278. (Biology). 查看参考
      7. Hennersdorf F, Florian S, Jakob A, et al.. Identification of CD13, CD107a, and CD164 as novel basophil-activation markers and dissection of two response patterns in time kinetics of IgE-dependent upregulation. Cell Res. 2005; 15(5):325-335. (Biology). 查看参考
      8. Iwamoto T, Yuta A, Tabata T, et al. Evaluation of basophil CD203c as a predictor of carboplatin-related hypersensitivity reaction in patients with gynecologic cancer. Biol Pharm Bull. 2012; 35(9):1487-1495. (Biology). 查看参考
      查看所有文件 (8) 查看更少内容
      770472 Rev. 1

       

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      Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

      For Research Use Only. Not for use in diagnostic or therapeutic procedures.