Skip to main content Skip to navigation
Hamburger Menu
Search icon
Country Selector Country Selector
China (Chinese)
产品

解决方案

发现&学习

资源&工具

Support

Search icon Search icon
Close Menu
      Alert Icon
      RB545 Mouse Anti-Human IgA1
      商品详情
      Down Arrow Up Arrow


      BD OptiBuild™
      IGHA1; Immunoglobulin alpha 1
      Human (Tested in Development)
      Mouse BALB/c IgG1, κ
      Human IgA1 Myeloma Protein
      Flow cytometry (Qualified)
      0.2 mg/ml
      3493
      Aqueous buffered solution containing ≤0.09% sodium azide.
      RUO


      准备和存储

      Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to the dye under optimum conditions that minimize unconjugated dye and antibody.
      显示较多信息 blue down arrow 显示较少信息 blue up arrow

      推荐的实验流程

      BD® CompBeads can be used as surrogates to assess fluorescence spillover (compensation). When fluorochrome conjugated antibodies are bound to BD® CompBeads, they have spectral properties very similar to cells. However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls. It is strongly recommended that when using a reagent for the first time, users compare the spillover on cells and BD® CompBeads to ensure that BD® CompBeads are appropriate for your specific cellular application.

      显示较多信息 blue down arrow 显示较少信息 blue up arrow

      商品通知

      1. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
      2. Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
      3. For U.S. patents that may apply, see bd.com/patents.
      4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
      5. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
      6. The production process underwent stringent testing and validation to assure that it generates a high-quality conjugate with consistent performance and specific binding activity. However, verification testing has not been performed on all conjugate lots.
      7. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
      8. An isotype control should be used at the same concentration as the antibody of interest.
      9. Please observe the following precautions: We recommend that special precautions be taken (such as wrapping vials, tubes, or racks in aluminum foil) to protect exposure of conjugated reagents, including cells stained with those reagents, to any room illumination. Absorption of visible light can significantly affect the emission spectra and quantum yield of tandem fluorochrome conjugates.
      10. Human donor specific background has been observed in relation to the presence of anti-polyethylene glycol (PEG) antibodies, developed as a result of certain vaccines containing PEG, including some COVID-19 vaccines. We recommend use of BD Horizon Brilliant™ Stain Buffer in your experiments to help mitigate potential background. For more information visit https://www.bdbiosciences.com/en-us/support/product-notices.
      显示较多信息 blue down arrow 显示较少信息 blue up arrow
      772216 Rev. 1
      抗体详情
      Down Arrow Up Arrow
      B3506B4

      The B3506B4 monoclonal antibody specifically recognizes the Fc region of the human Immunoglobulin A (IgA) subclass known as IgA1. IgA1 is comprised of two identical heavy chains encoded by IGHA1, and two light chains, either Igκ or Igλ, that are linked together by disulfide bonds. The B3506B4 antibody does not crossreact with other human immunoglobulin heavy chain (IgH) subclasses, including the IgA2 subclass. IgA1 is expressed by plasmablasts, plasma cells, and memory B cells as well as by some myeloma or plasmacytoma cells. IgA1 is expressed in either a transmembrane form that serves as an antigen-specific B cell surface receptor or in a soluble secreted form. Monomeric and dimeric forms of IgA1 are found in the serum although the monomeric form predominates. Multimeric forms of IgA1 connected by a J-chain and secretory component are also found in bodily secretions. Secretory IgA is transported across epithelial cells and secreted into the lumen of mucosal tissues including the gastrointestinal and respiratory tracts and is found in human breast milk. IgA1 functions in the agglutination of microbes and can block their adherence to or infection of human cells. It can also bind to CD89 (FcαRI) expressed on neutrophils, monocytes, macrophages, and eosinophils to trigger a variety of immune responses including phagocytosis, antibody-dependent cell-mediated cytotoxicity, and the release of inflammatory cytokines and mediators such as in the response to microbes.

      772216 Rev. 1
      格式详情
      Down Arrow Up Arrow
      RB545
      The BD Horizon RealBlue™ 545 (RB545) fluorochrome is part of the BD family of blue (488-nm) laser dyes. It is a small organic fluorochrome with an excitation maximum (Ex Max) at 496-nm and an emission maximum (Em Max) at 545-nm. Driven by BD innovation, RB545 can be used on spectral cytometers and is designed to be excited by the blue (488-nm) laser with minimal excitation by the 561-nm yellow-green laser. RB545 has minimal spillover into yellow-green detectors and a brightness level similar to FITC.  Given its unique emission max, on a spectral instrument RB545 can be used simultaneously with FITC and PE to provide an additional color off of the blue laser. Please ensure that your instrument configuration (lasers and optical filters) is appropriate for this dye.
      altImg
      RB545
      Blue 488 nm
      496 nm
      545 nm
      772216 Rev.1
      报价单和参考
      Down Arrow Up Arrow
      View product citations for antibody "772216" on CiteAb

      研发参考 (9)

      1. Avery DT, Bryant VL, Ma CS, de Waal Malefyt R, Tangye SG. IL-21-induced isotype switching to IgG and IgA by human naive B cells is differentially regulated by IL-4.. J Immunol. 2008; 181(3):1767-79. (Clone-specific: Flow cytometry). 查看参考
      2. Blanco E, Perez-Andres M, Sanoja-Flores L, et al. Selection and validation of antibody clones against IgG and IgA subclasses in switched memory B-cells and plasma cells.. J Immunol Methods. 2019; 475:112372. (Clone-specific: Flow cytometry). 查看参考
      3. Blanco E, Pérez-Andrés M, Arriba-Méndez S, et al. Age-associated distribution of normal B-cell and plasma cell subsets in peripheral blood.. J Allergy Clin Immunol. 2018; 141(6):2208-2219.e16. (Clone-specific: Flow cytometry). 查看参考
      4. Delacroix DL, Van Snick J, Vaerman JP, Conley ME, Mascart-Lemone F, Bernier GM. Monoclonal antibodies against isotypic and isoallotypic determinants of human IgA1 and IgA2: fine specificities and binding properties.. Mol Immunol. 1986; 23(4):367-75. (Immunogen: Radioimmunoassay). 查看参考
      5. Fayette J, Dubois B, Vandenabeele S, et al. Human dendritic cells skew isotype switching of CD40-activated naive B cells towards IgA1 and IgA2.. J Exp Med. 1997; 185(11):1909-18. (Biology). 查看参考
      6. He B, Xu W, Santini PA, et al. Intestinal bacteria trigger T cell-independent immunoglobulin A(2) class switching by inducing epithelial-cell secretion of the cytokine APRIL.. Immunity. 2007; 26(6):812-26. (Clone-specific: Flow cytometry). 查看参考
      7. Sibille Y, Chatelain B, Staquet P, Merrill WW, Delacroix DL, Vaerman JP. Surface IgA and Fc-alpha receptors on human alveolar macrophages from normal subjects and from patients with sarcoidosis.. Am Rev Respir Dis. 1989; 139(3):740-7. (Clone-specific: Blocking). 查看参考
      8. Tangye SG, Ferguson A, Avery DT, Ma CS, Hodgkin PD. Isotype switching by human B cells is division-associated and regulated by cytokines.. J Immunol. 2002; 169(8):4298-306. (Biology). 查看参考
      9. Zan H, Cerutti A, Dramitinos P, Schaffer A, Casali P. CD40 engagement triggers switching to IgA1 and IgA2 in human B cells through induction of endogenous TGF-beta: evidence for TGF-beta but not IL-10-dependent direct S mu-->S alpha and sequential S mu-->S gamma, S gamma-->S alpha DNA recombination.. J Immunol. 1998; 161(10):5217-25. (Biology). 查看参考
      查看所有文件 (9) 查看更少内容
      772216 Rev. 1

       

      Please refer to Support Documents for Quality Certificates


      Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

      For Research Use Only. Not for use in diagnostic or therapeutic procedures.