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      R718 Rat Anti-Mouse CD140b (PDGFRβ)
      R718 Rat Anti-Mouse CD140b (PDGFRβ)
      Multicolor flow cytometric analysis of the correlated expression of CD140a and CD140b (PDGFRβ) on Mouse NIH/3T3 cells.  Cells from the Mouse NIH/3T3 (Mouse embryonic fibroblast, ATCC® CRL-1658™) cell line were stained with BD Horizon™ R718 Rat IgG2a, κ Isotype Control (Cat. No. 566941) and PE Rat IgG2a, κ Isotype Control (Cat. No. 553930) [Left Plot] or with BD Horizon™ R718 Rat Anti-Mouse CD140b (PDGFRβ) antibody (Cat. No. 570046/570127) and PE Rat Anti-Mouse CD140a antibody (Cat. No. 562776) [Right Plot] at 0.25 µg/test. DAPI (4',6-Diamidino-2-Phenylindole, Dihydrochloride) Solution (Cat. No. 564907) was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD140b (PDGFRβ) versus CD140a (or Ig Isotype control staining) was derived from gated events with the forward and side light-scatter characteristics of viable (DAPI-negative) cells. Flow cytometry and data analysis were performed using a BD LSRFortessa™ X-20 Cell Analyzer System and FlowJo™ Software. Data shown on this Technical Data Sheet are not lot specific.
      R718 Rat Anti-Mouse CD140b (PDGFRβ)
      Flow cytometric analysis of CD140b (PDGFRβ) expression on Mouse splenic leukocytes and Mouse NIH/3T3 cells.  C57BL/6 Mouse splenocytes (Left Plot) were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody (Mouse BD Fc Block™) [Cat. No. 553141/553142]. The splenic leukocytes and cells from the Mouse NIH/3T3 (Mouse embryonic fibroblast, ATCC® CRL-1658™) cell line (Right Plot) were then stained with either BD Horizon™ R718 Rat IgG2a, κ Isotype Control (Cat. No. 566941; dashed line histograms) or BD Horizon™ R718 Rat Anti-Mouse CD140b (PDGFRβ) antibody (Cat. No. 570046/570127; solid line histograms) at 0.25 µg/test. DAPI (4',6-Diamidino-2-Phenylindole, Dihydrochloride) Solution (Cat. No. 564907) was added to cells right before analysis. The fluorescence histograms showing CD140b (PDGFRβ) expression (or Ig Isotype control staining) were derived from gated events with the forward and side light-scatter characteristics of viable (DAPI-negative) cells. Flow cytometry and data analysis were performed using a BD LSRFortessa™ X-20 Cell Analyzer System and FlowJo™ Software. Data shown on this Technical Data Sheet are not lot specific.
      R718 Rat Anti-Mouse CD140b (PDGFRβ) R718 Rat Anti-Mouse CD140b (PDGFRβ)
      Multicolor flow cytometric analysis of the correlated expression of CD140a and CD140b (PDGFRβ) on Mouse NIH/3T3 cells.  Cells from the Mouse NIH/3T3 (Mouse embryonic fibroblast, ATCC® CRL-1658™) cell line were stained with BD Horizon™ R718 Rat IgG2a, κ Isotype Control (Cat. No. 566941) and PE Rat IgG2a, κ Isotype Control (Cat. No. 553930) [Left Plot] or with BD Horizon™ R718 Rat Anti-Mouse CD140b (PDGFRβ) antibody (Cat. No. 570046/570127) and PE Rat Anti-Mouse CD140a antibody (Cat. No. 562776) [Right Plot] at 0.25 µg/test. DAPI (4',6-Diamidino-2-Phenylindole, Dihydrochloride) Solution (Cat. No. 564907) was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD140b (PDGFRβ) versus CD140a (or Ig Isotype control staining) was derived from gated events with the forward and side light-scatter characteristics of viable (DAPI-negative) cells. Flow cytometry and data analysis were performed using a BD LSRFortessa™ X-20 Cell Analyzer System and FlowJo™ Software. Data shown on this Technical Data Sheet are not lot specific.
      Flow cytometric analysis of CD140b (PDGFRβ) expression on Mouse splenic leukocytes and Mouse NIH/3T3 cells.  C57BL/6 Mouse splenocytes (Left Plot) were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody (Mouse BD Fc Block™) [Cat. No. 553141/553142]. The splenic leukocytes and cells from the Mouse NIH/3T3 (Mouse embryonic fibroblast, ATCC® CRL-1658™) cell line (Right Plot) were then stained with either BD Horizon™ R718 Rat IgG2a, κ Isotype Control (Cat. No. 566941; dashed line histograms) or BD Horizon™ R718 Rat Anti-Mouse CD140b (PDGFRβ) antibody (Cat. No. 570046/570127; solid line histograms) at 0.25 µg/test. DAPI (4',6-Diamidino-2-Phenylindole, Dihydrochloride) Solution (Cat. No. 564907) was added to cells right before analysis. The fluorescence histograms showing CD140b (PDGFRβ) expression (or Ig Isotype control staining) were derived from gated events with the forward and side light-scatter characteristics of viable (DAPI-negative) cells. Flow cytometry and data analysis were performed using a BD LSRFortessa™ X-20 Cell Analyzer System and FlowJo™ Software. Data shown on this Technical Data Sheet are not lot specific.
      商品详情
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      BD Horizon™
      CD140b; PDGF-R-beta; PDGFR-beta; Pdgfrb; platelet-derived growth factor receptor beta
      Mouse (QC Testing)
      Rat WI, also known as Wistar (outbred) IgG2a, κ
      Recombinant Mouse PDGFRβ Extracellular Domain Protein
      Flow cytometry (Routinely Tested)
      0.2 mg/ml
      18596
      Aqueous buffered solution containing ≤0.09% sodium azide.
      RUO


      准备和存储

      The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to the dye under optimum conditions and unreacted dye was removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
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      推荐的实验流程

      BD® CompBeads can be used as surrogates to assess fluorescence spillover (compensation). When fluorochrome conjugated antibodies are bound to BD® CompBeads, they have spectral properties very similar to cells. However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls. It is strongly recommended that when using a reagent for the first time, users compare the spillover on cells and BD® CompBeads to ensure that BD® CompBeads are appropriate for your specific cellular application.

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      商品通知

      1. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
      2. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
      3. An isotype control should be used at the same concentration as the antibody of interest.
      4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
      5. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
      6. Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
      7. This product is provided under an Agreement between BIOTIUM and BD Biosciences. This product, and only in the amount purchased by buyer, may be used solely for buyer’s own internal research, in a manner consistent with the accompanying product literature. No other right to use, sell or otherwise transfer (a) this product, or (b) its components is hereby granted expressly, by implication or by estoppel. This product is for research use only. Diagnostic uses require a separate license from Biotium, Inc. For information on purchasing a license to this product including for purposes other than research, contact Biotium, Inc., 3159 Corporate Place, Hayward, CA 94545, Tel: (510) 265-1027. Fax: (510) 265-1352. Email: btinfo@biotium.com.
      8. Alexa Fluor™ is a trademark of Life Technologies Corporation.
      9. For U.S. patents that may apply, see bd.com/patents.
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      570127 Rev. 1
      抗体详情
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      APB5

      The APB5 monoclonal antibody specifically recognizes CD140b, the Platelet-Derived Growth Factor Receptor beta chain (PDGFRβ). CD140b is a single-pass type I transmembrane glycoprotein that contains an intracellular tyrosine kinase domain. This receptor is encoded by Pdgfrb and is widely expressed on cells within embryonic tissues and cells of mesenchymal origin in the adult mouse including fibroblasts and vascular smooth muscle cells. CD140b forms homodimers or heterodimeric receptor complexes with CD140a (PDGFRα) upon binding various dimeric Platelet-Derived Growth Factors (PDGF) isoforms that lead to receptor activation. Upon activation, CD140b is autophosphorylated at multiple tyrosine sites and, in turn, initiates several signaling cascades by binding and activation of cytoplasmic SH2 domain-containing signal transduction molecules, such as GRB2, Src, GAP, PI3 kinase, PLCγ, and NCK. These signaling pathways regulate cellular growth and proliferation, survival, differentiation, and migration involved in the development of embryonic tissues, blood vessel formation, wound healing, and in a range of diseases including fibrotic conditions, atherosclerosis, and malignancies.

      570127 Rev. 1
      格式详情
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      R718
      The BD Horizon™ Red 718 (R718) Dye is part of the BD red family of dyes. It is a small organic fluorochrome with an excitation maximum (Ex Max) at 695-nm and an emission maximum (Em Max) at 718-nm. Driven by BD innovation, R718 is designed to be excited by the red laser (627–640-nm) and detected using an optical filter centered near 720-nm (e.g., a 720/40-nm bandpass filter). R718 is a brighter alternative to Alexa Fluor™ 700. R718 is also a bright small molecule alternative to APC-R700 with lower spread into the APC detector. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
      altImg
      R718
      Red 627-640 nm
      695 nm
      718 nm
      570127 Rev.1
      报价单和参考
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      View product citations for antibody "570127" on CiteAb

      研发参考 (7)

      1. Andrae J, Gallini R, Betsholtz C. Role of platelet-derived growth factors in physiology and medicine.. Genes Dev. 2008; 22(10):1276-312. (Biology). 查看参考
      2. Cossarizza A, Chang HD, Radbruch A, et al. Guidelines for the use of flow cytometry and cell sorting in immunological studies (second edition).. Eur J Immunol. 2019; 49(10):1457-1973. (Clone-specific). 查看参考
      3. Cremasco V, Woodruff MC, Onder L, et al. B cell homeostasis and follicle confines are governed by fibroblastic reticular cells.. Nat Immunol. 2014; 15(10):973-81. (Clone-specific: Flow cytometry). 查看参考
      4. Ostman A, Heldin CH. Involvement of platelet-derived growth factor in disease: development of specific antagonists.. Adv Cancer Res. 2001; 80:1-38. (Biology). 查看参考
      5. Patenaude J, Perreault C. Thymic Mesenchymal Cells Have a Distinct Transcriptomic Profile.. J Immunol. 2016; 196(11):4760-70. (Clone-specific: Flow cytometry). 查看参考
      6. Sano H, Sudo T, Yokode M, et al. Functional blockade of platelet-derived growth factor receptor-beta but not of receptor-alpha prevents vascular smooth muscle cell accumulation in fibrous cap lesions in apolipoprotein E-deficient mice.. Circulation. 2001; 103(24):2955-60. (Immunogen: Functional assay, Immunohistochemistry, Western blot). 查看参考
      7. Sano H, Yokode M, Takakura N, et al. Study on PDGF receptor beta pathway in glomerular formation in neonate mice.. Ann N Y Acad Sci. 2001; 947:303-5. (Clone-specific: Immunohistochemistry, Western blot). 查看参考
      查看所有文件 (7) 查看更少内容
      570127 Rev. 1

      Please refer to Support Documents for Quality Certificates


      Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

      For Research Use Only. Not for use in diagnostic or therapeutic procedures.