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      R718 Mouse Anti-Human Granzyme K
      R718 Mouse Anti-Human Granzyme K
      Two-color flow cytometric analysis of Granzyme K expression in Human peripheral blood lymphocytes.  Human peripheral blood cells were treated (15 min; room temperature) with BD Phosflow™ Lyse/Fix Buffer (Cat. No. 558049) to lyse erythrocytes and fix leukocytes. The leukocytes were washed and then stained in BD Perm/Wash™ Buffer (Cat. No. 554723) with BD Horizon™ BV421 Mouse Anti-Human CD8 antibody (Cat. No. 562428) and with either BD Horizon™ R718 Mouse IgG2a, κ Isotype Control (Cat. No. 567089; Left Plot) or BD Horizon™ R718 Mouse Anti-Human Granzyme K antibody (Cat. No. 570343/570413; Right Plot). The pseudocolor density plot showing the correlated expression of Granzyme K (or Ig Isotype control staining) versus CD8 was derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa™ Cell Analyzer System and FlowJo™ Software.
      R718 Mouse Anti-Human Granzyme K
      Two-color flow cytometric analysis of Granzyme K expression in Human peripheral blood lymphocytes.  Human peripheral blood cells were treated (15 min; room temperature) with BD Phosflow™ Lyse/Fix Buffer (Cat. No. 558049) to lyse erythrocytes and fix leukocytes. The leukocytes were washed and then stained in BD Perm/Wash™ Buffer (Cat. No. 554723) with BD Horizon™ BV421 Mouse Anti-Human CD8 antibody (Cat. No. 562428) and with either BD Horizon™ R718 Mouse IgG2a, κ Isotype Control (Cat. No. 567089; Left Plot) or BD Horizon™ R718 Mouse Anti-Human Granzyme K antibody (Cat. No. 570343/570413; Right Plot). The pseudocolor density plot showing the correlated expression of Granzyme K (or Ig Isotype control staining) versus CD8 was derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa™ Cell Analyzer System and FlowJo™ Software.
      商品详情
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      BD Horizon™
      GZMK; GRAK; TRYP2; fragmentin-3; granzyme 3; tryptase II
      Human (QC Testing)
      Mouse BALB/c IgG2a, κ
      Human Granzyme K Recombinant Protein
      Intracellular staining (flow cytometry) (Routinely Tested)
      5 µl/test
      3003
      Aqueous buffered solution containing ≤0.09% sodium azide.
      RUO


      准备和存储

      The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to the dye under optimum conditions and unreacted dye was removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
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      推荐的实验流程

      BD® CompBeads can be used as surrogates to assess fluorescence spillover (compensation). When fluorochrome conjugated antibodies are bound to BD® CompBeads, they have spectral properties very similar to cells. However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls. It is strongly recommended that when using a reagent for the first time, users compare the spillover on cells and BD® CompBeads to ensure that BD® CompBeads are appropriate for your specific cellular application.

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      商品通知

      1. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
      2. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
      3. This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
      4. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
      5. An isotype control should be used at the same concentration as the antibody of interest.
      6. Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
      7. This product is provided under an Agreement between BIOTIUM and BD Biosciences. This product, and only in the amount purchased by buyer, may be used solely for buyer’s own internal research, in a manner consistent with the accompanying product literature. No other right to use, sell or otherwise transfer (a) this product, or (b) its components is hereby granted expressly, by implication or by estoppel. This product is for research use only. Diagnostic uses require a separate license from Biotium, Inc. For information on purchasing a license to this product including for purposes other than research, contact Biotium, Inc., 3159 Corporate Place, Hayward, CA 94545, Tel: (510) 265-1027. Fax: (510) 265-1352. Email: btinfo@biotium.com.
      8. Human donor specific background has been observed in relation to the presence of anti-polyethylene glycol (PEG) antibodies, developed as a result of certain vaccines containing PEG, including some COVID-19 vaccines. We recommend use of BD Horizon Brilliant™ Stain Buffer in your experiments to help mitigate potential background. For more information visit https://www.bdbiosciences.com/en-us/support/product-notices.
      9. Alexa Fluor™ is a trademark of Life Technologies Corporation.
      10. For U.S. patents that may apply, see bd.com/patents.
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      570413 Rev. 1
      抗体详情
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      G3H69

      The G3H69 monoclonal antibody specifically recognizes Granzyme K which is also known as GRAK, Granzyme-3, Fragmentin-3, Tryptase II (TRYP2), or NK-tryptase-2 (NK-Tryp-2). This granule-associated enzyme is a serine protease that cleaves peptides after the basic residues lysine and arginine. Granzyme K is encoded by GZMK (granzyme K) that belongs to the Granzyme subfamily (Granzymes A, B, H, K, M) within the peptidase S1 family. Granzyme K is expressed in the granules of CD56-bright natural killer (NK) cells, NKT cells, γδ T cells, and cytotoxic CD4+ T cells and CD8+ T cells that can kill pathogen-infected or tumor cells. Granzyme K can activate the SET complex enabling DNase NM23-H1 to make single-stranded nicks in chromosomal DNA which ultimately contribute to target cell death. Granzyme K can also act on Bid and p53 to activate certain cell death pathways. Elevated levels of Granzyme K have been observed in the blood and bodily fluids from individuals under certain infectious or inflammatory conditions.

      570413 Rev. 1
      格式详情
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      R718
      The BD Horizon™ Red 718 (R718) Dye is part of the BD red family of dyes. It is a small organic fluorochrome with an excitation maximum (Ex Max) at 695-nm and an emission maximum (Em Max) at 718-nm. Driven by BD innovation, R718 is designed to be excited by the red laser (627–640-nm) and detected using an optical filter centered near 720-nm (e.g., a 720/40-nm bandpass filter). R718 is a brighter alternative to Alexa Fluor™ 700. R718 is also a bright small molecule alternative to APC-R700 with lower spread into the APC detector. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
      altImg
      R718
      Red 627-640 nm
      695 nm
      718 nm
      570413 Rev.1
      报价单和参考
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      View product citations for antibody "570413" on CiteAb

      研发参考 (6)

      1. Bade B, Boettcher HE, Lohrmann J, et al. Differential expression of the granzymes A, K and M and perforin in human peripheral blood lymphocytes.. Int Immunol. 2005; 17(11):1419-28. (Biology). 查看参考
      2. Baracho GV, Kara N, Rigaud S, Lo E, Widmann SJ, Tyznik AJ. Functional phenotyping of circulating human cytotoxic T cells and NK cells using a 16-color flow cytometry panel.. STAR Protoc. 2022; 3(1):101069. (Clone-specific: Flow cytometry). 查看参考
      3. Hameed A, Lowrey DM, Lichtenheld M, Podack ER. Characterization of three serine esterases isolated from human IL-2 activated killer cells. J Immunol. 1988; 141:3142-3147. (Biology). 查看参考
      4. Inagaki H, Hirata Y, Shimizu T, Kobayashi M, Li Q, Kawada T. Expression of granzyme 3 protein in human peripheral blood lymphocytes analyzed by flow cytometry (PP-108-30). Int Immunol (Abstract). 2010; 22:172. (Immunogen: Flow cytometry).
      5. Lieberman J. The ABCs of granule-mediated cytotoxicity: new weapons in the arsenal.. Nat Rev Immunol. 2003; 3(5):361-70. (Biology). 查看参考
      6. Wilharm E, Parry MA, Friebel R, et al. Generation of catalytically active granzyme K from Escherichia coli inclusion bodies and identification of efficient granzyme K inhibitors in human plasma. J Biol Chem. 1999; 274:27331-27337. (Biology). 查看参考
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      570413 Rev. 1

       

      Please refer to Support Documents for Quality Certificates


      Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

      For Research Use Only. Not for use in diagnostic or therapeutic procedures.