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R718 Mouse Anti-Human Chondroitin Sulfate (NG2)
商品详情
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BD OptiBuild™
CSPG4; HMW-MAA; MCSP; MCSPG; NG2
Human (Tested in Development)
Mouse IgG1, κ
Human BM stromal cell line established by infection with SV-40
Flow cytometry (Qualified)
0.2 mg/ml
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO


准备和存储

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to the dye under optimum conditions that minimize unconjugated dye and antibody.

推荐的实验流程

BD® CompBeads can be used as surrogates to assess fluorescence spillover (compensation). When fluorochrome conjugated antibodies are bound to BD® CompBeads, they have spectral properties very similar to cells. However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls. It is strongly recommended that when using a reagent for the first time, users compare the spillover on cells and BD® CompBeads to ensure that BD® CompBeads are appropriate for your specific cellular application.

商品通知

  1. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  2. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  3. An isotype control should be used at the same concentration as the antibody of interest.
  4. Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
  5. This product is provided under an Agreement between BIOTIUM and BD Biosciences. This product, and only in the amount purchased by buyer, may be used solely for buyer’s own internal research, in a manner consistent with the accompanying product literature. No other right to use, sell or otherwise transfer (a) this product, or (b) its components is hereby granted expressly, by implication or by estoppel. This product is for research use only. Diagnostic uses require a separate license from Biotium, Inc. For information on purchasing a license to this product including for purposes other than research, contact Biotium, Inc., 3159 Corporate Place, Hayward, CA 94545, Tel: (510) 265-1027. Fax: (510) 265-1352. Email: btinfo@biotium.com.
  6. Alexa Fluor™ is a trademark of Life Technologies Corporation.
  7. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  8. Human donor specific background has been observed in relation to the presence of anti-polyethylene glycol (PEG) antibodies, developed as a result of certain vaccines containing PEG, including some COVID-19 vaccines. We recommend use of BD Horizon Brilliant™ Stain Buffer in your experiments to help mitigate potential background. For more information visit https://www.bdbiosciences.com/en-us/support/product-notices.
  9. Researchers should determine the optimal concentration of this reagent for their individual applications.
  10. The production process underwent stringent testing and validation to assure that it generates a high-quality conjugate with consistent performance and specific binding activity. However, verification testing has not been performed on all conjugate lots.
756719 Rev. 1
抗体详情
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7.1

The 7.1 monoclonal antibody specifically recognizes Neural/Glial Antigen 2 (NG2) which is also known as Melanoma chondroitin sulfate proteoglycan (MCSP) and High molecular weight-melanoma associated antigen (HMW-MAA). This ~251 kDa transmembrane proteoglycan is encoded by CSPG4 (Chondroitin sulfate proteoglycan 4). Chondroitin Sulfate (NG2) is expressed by glial cells but not detected on normal marrow hematopoietic and peripheral blood cells. It is highly expressed by cells in some cancers including melanomas, leukemias, sarcomas and carcinomas. Chondroitin Sulfate (NG2) plays important roles in cellular proliferation, migration, and in oncogenic pathways involved in malignant transformation and metastases.  

756719 Rev. 1
格式详情
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R718
The BD Horizon™ Red 718 (R718) Dye is part of the BD red family of dyes. It is a small organic fluorochrome with an excitation maximum (Ex Max) at 695-nm and an emission maximum (Em Max) at 718-nm. Driven by BD innovation, R718 is designed to be excited by the red laser (627–640-nm) and detected using an optical filter centered near 720-nm (e.g., a 720/40-nm bandpass filter). R718 is a brighter alternative to Alexa Fluor™ 700. R718 is also a bright small molecule alternative to APC-R700 with lower spread into the APC detector. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
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R718
Red 627-640 nm
695 nm
718 nm
756719 Rev.1
报价单和参考
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View product citations for antibody "756719" on CiteAb

研发参考 (5)

  1. Behm FG, Smith FO, Raimondi SC, Pui CH, Bernstein ID. Human homologue of the rat chondroitin sulfate proteoglycan, NG2, detected by monoclonal antibody 7.1, identifies childhood acute lymphoblastic leukemias with t(4;11)(q21;q23) or t(11;19)(q23;p13) and MLL gene rearrangements.. Blood. 1996; 87(3):1134-9. (Immunogen: Flow cytometry). 查看参考
  2. Luo W, Wang X, Kageshita T, Wakasugi S, Karpf AR, Ferrone S. Regulation of high molecular weight-melanoma associated antigen (HMW-MAA) gene expression by promoter DNA methylation in human melanoma cells.. Oncogene. 2006; 25(20):2873-84. (Biology). 查看参考
  3. Pluschke G, Vanek M, Evans A, et al. Molecular cloning of a human melanoma-associated chondroitin sulfate proteoglycan. Proc Natl Acad Sci U S A. 1996; 93(18):9710-9715. (Biology). 查看参考
  4. Tamburini E, Dallatomasina A, Quartararo J, et al. Structural deciphering of the NG2/CSPG4 proteoglycan multifunctionality.. FASEB J. 2019; 33(3):3112-3128. (Biology). 查看参考
  5. Wang X, Wang Y, Yu L, et al. CSPG4 in cancer: multiple roles.. Curr Mol Med. 2010; 10(4):419-29. (Biology). 查看参考
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756719 Rev. 1

 

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For Research Use Only. Not for use in diagnostic or therapeutic procedures.