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      R718 Mouse Anti-Human CD79a
      R718 Mouse Anti-Human CD79a
      Flow cytometric analysis of CD79a expression in Human peripheral blood lymphocytes.  Human peripheral blood mononuclear cells (PBMCs) were fixed with BD Cytofix™ Fixation Buffer (Cat. No. 554655) and permeabilized with BD Perm/Wash™ Buffer (Cat. No. 554723). The cells were subsequently stained with either BD Horizon™ R718 Mouse IgG1, κ Isotype Control (Cat. No. 566928; dashed line histogram) or BD Horizon™ R718 Mouse Anti-Human CD79a antibody (Cat. No. 570952/571023; solid line histogram). The fluorescence histogram showing CD79a expression (or Ig Isotype control staining) was derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometric analysis was performed using a BD LSRFortessa™ X-20 Cell Analyzer System and FlowJo™ Software.
      R718 Mouse Anti-Human CD79a
      Multiparameter flow cytometric analysis of CD79a expression in Human peripheral mononuclear cells.  Human peripheral mononuclear cells (PBMCs) were fixed with BD Cytofix™ Fixation Buffer (Cat. No. 554655) and permeabilized with BD Perm/Wash™ Buffer (Cat. No. 554723). The cells were subsequently stained with either BD Horizon™ R718 Mouse IgG1, κ Isotype Control (Cat. No. 566928; Left Plot) or BD Horizon™ R718 Mouse Anti-Human CD79a antibody (Cat. No. 570952/571023; Right Plot). The bivariate pseudocolor density plot showing the correlated expression of CD79a (or Ig Isotype control staining) versus side light-scatter (SSC-A) signals was derived from gated events with the forward and side light-scatter characteristics of intact PBMCs. Flow cytometry and data analysis were performed using a BD LSRFortessa™ X-20 Cell Analyzer System and FlowJo™ Software.
      R718 Mouse Anti-Human CD79a R718 Mouse Anti-Human CD79a
      Flow cytometric analysis of CD79a expression in Human peripheral blood lymphocytes.  Human peripheral blood mononuclear cells (PBMCs) were fixed with BD Cytofix™ Fixation Buffer (Cat. No. 554655) and permeabilized with BD Perm/Wash™ Buffer (Cat. No. 554723). The cells were subsequently stained with either BD Horizon™ R718 Mouse IgG1, κ Isotype Control (Cat. No. 566928; dashed line histogram) or BD Horizon™ R718 Mouse Anti-Human CD79a antibody (Cat. No. 570952/571023; solid line histogram). The fluorescence histogram showing CD79a expression (or Ig Isotype control staining) was derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometric analysis was performed using a BD LSRFortessa™ X-20 Cell Analyzer System and FlowJo™ Software.
      Multiparameter flow cytometric analysis of CD79a expression in Human peripheral mononuclear cells.  Human peripheral mononuclear cells (PBMCs) were fixed with BD Cytofix™ Fixation Buffer (Cat. No. 554655) and permeabilized with BD Perm/Wash™ Buffer (Cat. No. 554723). The cells were subsequently stained with either BD Horizon™ R718 Mouse IgG1, κ Isotype Control (Cat. No. 566928; Left Plot) or BD Horizon™ R718 Mouse Anti-Human CD79a antibody (Cat. No. 570952/571023; Right Plot). The bivariate pseudocolor density plot showing the correlated expression of CD79a (or Ig Isotype control staining) versus side light-scatter (SSC-A) signals was derived from gated events with the forward and side light-scatter characteristics of intact PBMCs. Flow cytometry and data analysis were performed using a BD LSRFortessa™ X-20 Cell Analyzer System and FlowJo™ Software.
      商品详情
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      BD Horizon™
      CD79A; CD79α; Immunoglobulin-associated alpha; Ig-alpha; IGA; Igα; MB-1
      Human (QC Testing), Mouse,Rat,Monkey,Pig,Dog,Chicken,Rabbit,Cow,Guinea Pig,Horse (Reported)
      Mouse BALB/c IgG1, κ
      Human CD79a Peptide
      Intracellular staining (flow cytometry) (Routinely Tested)
      5 µl/test
      V cB018; VI B103
      Aqueous buffered solution containing ≤0.09% sodium azide.
      RUO


      准备和存储

      The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to the dye under optimum conditions and unreacted dye was removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
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      推荐的实验流程

      BD® CompBeads can be used as surrogates to assess fluorescence spillover (compensation). When fluorochrome conjugated antibodies are bound to BD® CompBeads, they have spectral properties very similar to cells. However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls. It is strongly recommended that when using a reagent for the first time, users compare the spillover on cells and BD® CompBeads to ensure that BD® CompBeads are appropriate for your specific cellular application.

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      商品通知

      1. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
      2. An isotype control should be used at the same concentration as the antibody of interest.
      3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
      4. This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
      5. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
      6. Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
      7. Human donor specific background has been observed in relation to the presence of anti-polyethylene glycol (PEG) antibodies, developed as a result of certain vaccines containing PEG, including some COVID-19 vaccines. We recommend use of BD Horizon Brilliant™ Stain Buffer in your experiments to help mitigate potential background. For more information visit https://www.bdbiosciences.com/en-us/support/product-notices.
      8. This product is provided under an Agreement between BIOTIUM and BD Biosciences. This product, and only in the amount purchased by buyer, may be used solely for buyer’s own internal research, in a manner consistent with the accompanying product literature. No other right to use, sell or otherwise transfer (a) this product, or (b) its components is hereby granted expressly, by implication or by estoppel. This product is for research use only. Diagnostic uses require a separate license from Biotium, Inc. For information on purchasing a license to this product including for purposes other than research, contact Biotium, Inc., 3159 Corporate Place, Hayward, CA 94545, Tel: (510) 265-1027. Fax: (510) 265-1352. Email: btinfo@biotium.com.
      9. Alexa Fluor™ is a trademark of Life Technologies Corporation.
      10. For U.S. patents that may apply, see bd.com/patents.
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      570952 Rev. 1
      抗体详情
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      HM57

      The HM57 monoclonal antibody specifically binds to the cytoplasmic domain of CD79a. CD79a is a 47-kDa type 1 transmembrane glycoprotein present on B lymphocytes. CD79a is also referred to as mb-1, IGA and Ig-alpha (Ig-α). It is expressed on B cells at various stages of differentiation, from the pre-B cell stage, probably before expression of cytoplasmic μ chain, to the plasma cell stage, in which it is detected only in the cytoplasm. CD79a associates with CD79b to form part of the B-cell receptor complex. It has been suggested that CD79a may play a role in mediating the transport of IgM to the cell surface. This antibody reportedly may crossreact with monkey, mouse, rat, bovine, canine, chicken, equine, guinea pig, porcine, and rabbit CD79a.

      570952 Rev. 1
      格式详情
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      R718
      The BD Horizon™ Red 718 (R718) Dye is part of the BD red family of dyes. It is a small organic fluorochrome with an excitation maximum (Ex Max) at 695-nm and an emission maximum (Em Max) at 718-nm. Driven by BD innovation, R718 is designed to be excited by the red laser (627–640-nm) and detected using an optical filter centered near 720-nm (e.g., a 720/40-nm bandpass filter). R718 is a brighter alternative to Alexa Fluor™ 700. R718 is also a bright small molecule alternative to APC-R700 with lower spread into the APC detector. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
      altImg
      R718
      Red 627-640 nm
      695 nm
      718 nm
      570952 Rev.1
      报价单和参考
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      View product citations for antibody "570952" on CiteAb

      研发参考 (6)

      1. Engel P, Wagner N, Tedder TF. CD79 Workshop report. In: Schlossman SF. Stuart F. Schlossman .. et al., ed. Leucocyte typing V : white cell differentiation antigens : proceedings of the fifth international workshop and conference held in Boston, USA, 3-7 November, 1993. Oxford: Oxford University Press; 1995:667-670.
      2. Faldyna M, Sinkora J, Knotigova P, Rehakova Z, Moravkova A, Toman M. Flow cytometric analysis of bone marrow leukocytes in neonatal dogs. Vet Immunol Immunopathol. 2003; 95(3-4):165-176. (Clone-specific: Flow cytometry). 查看参考
      3. Jones M, Cordell JL, Beyers AD, Tse AG, Mason DY. Detection of T and B cells in many animal species using cross-reactive anti-peptide antibodies. J Immunol. 1993; 150(12):5429-5435. (Clone-specific: Immunohistochemistry, Western blot). 查看参考
      4. Mason DY, Cordell JL, Tse AG, et al. The IgM-associated protein mb-1 as a marker of normal and neoplastic B cells. J Immunol. 1991; 147(11):2474-2482. (Immunogen: Flow cytometry, Immunoaffinity chromatography, Immunofluorescence, Immunohistochemistry, Immunoprecipitation). 查看参考
      5. Sakaguchi N, Kashiwamura S, Kimoto M, Thalmann P, Melchers F. B lymphocyte lineage-restricted expression of mb-1, a gene with CD3-like structural properties. EMBO J. 1988; 7(11):3457-3464. (Biology). 查看参考
      6. Schlossman SF. Stuart F. Schlossman .. et al., ed. Leucocyte typing V : white cell differentiation antigens : proceedings of the fifth international workshop and conference held in Boston, USA, 3-7 November, 1993. Oxford: Oxford University Press; 1995.
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      570952 Rev. 1

      Please refer to Support Documents for Quality Certificates

       

      Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

       

      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

      For Research Use Only. Not for use in diagnostic or therapeutic procedures.