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Purified Rat Anti-Human IL-12 p70
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BD Pharmingen™
Human (QC Testing)
Rat IgG1, κ
ELISA Capture (Routinely Tested), Intracellular block/flow cytometry (Tested During Development), Western blot (Reported)
0.5 mg/ml
Aqueous buffered solution containing ≤0.09% sodium azide.


ELISA Capture: The purified monoclonal antibody 20C2 (Cat. No. 555065) is useful as a capture antibody in a sandwich ELISA for measuring human IL-12 protein levels. Purified monoclonal antibody 20C2 can be paired with the biotinylated monoclonal antibody C8.6 (Cat. No. 554660) with recombinant human IL-12 p70 (Cat. No. 554613) as the standard. Purified monoclonal antibody 20C2 should be titrated to determine its optimal concentration for ELISA capture (2.0 µg/ml). To obtain linear standard curves, doubling dilutions of human IL-12 p70 ranging from ~2000 to 15 pg/ml are recommended for inclusion in each ELISA plate. For specific methodology, please visit the protocols section or chapter on ELISA in the Immune Function Handbook, both of which are posted on our web site,

Note 1: This ELISA pair is recommended primarily for measuring cytokines produced by experimental cell culture systems. These ELISA reagents are not recommended for measuring serum samples. The BD OptEIA™ Human IL-12 (p70) ELISA set (Cat. No. 555183) and Human IL-12 (p70) ELISA Kit (Cat. No. 559258) are specially-formulated for serum or plasma cytokine measurement.  

Note 2: This ELISA pair shows no cross-reactivity with any of the cytokines or chemokines tested  (including human IL-1α, IL-1β, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-10, IL-11, IL-13, IL-15, IL-16, eotaxin, G-CSF, GM-CSF, GROα, GROβ, GROγ, IFN-γ, IP-10, lymphotactin, MCP-1, MCP-2, MCP-3, MCP-4, MIP-1α, MIP-1β, NAP-2, PF-4, RANTES, TNF, LT-α).


  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to for technical protocols.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
555065 Rev. 2
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The 20C2 antibody reacts with the biologically active form of human IL-12 p70 heterodimer, but not with the p40 monomer or homodimer.

This antibody is routinely tested by ELISA Capture. Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.

555065 Rev. 2
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Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
555065 Rev.2
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View product citations for antibody "555065" on CiteAb

研发参考 (1)

  1. Gately MK, Chizzonite R, Presky DH. Measurement of Human and Mouse Interleukin-12. In: Cooligan J, Kruisbeek A, Margulies D, Shevach E, Storber W, ed. Current Protocols in Immunology. New York: John Wiley and Sons; 1995:6-16.
555065 Rev. 2

Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.