Skip to main content Skip to navigation
Hamburger Menu
Search icon
Country Selector Country Selector
China (Chinese)
登录/注册 UserName
产品

Link Arrow
解决方案

Link Arrow
发现&学习

Link Arrow
资源&工具

Link Arrow
Support

Link Arrow
Search icon Search icon
Close Menu
      Alert Icon
      Purified NA/LE Mouse Anti-Human Integrin α5 chain (CD49e)
      Purified NA/LE Mouse Anti-Human Integrin α5 chain (CD49e)
      Effects of anti-Integrin α5 chain (CD49e) antibodies on K562 cell binding to fibronectin. Aliquots of K562 cell suspension, an erythroleukemia cell line expressing Integrin α5 chain (CD49e) receptor, were incubated in the presence of the designated concentrations of Purified NA/LE Mouse Anti-Human Integrin α5 chain (CD49e) (Cat. No. 555614, • ) or Purified Mouse Anti-Human CD49e (Cat. No. 555651, □), for 30 minutes at room temperature. The suspensions were then transferred to plastic microtiter wells precoated with 12 µg/mL of fibronectin and incubated for 45 minutes at 37°C. Unattached cells were removed by aspiration and washing with PBS, and the attached cells were semi-quantitated using toluidine blue staining. The resulting absorbance signals are expressed relative to the average signal obtained without antibody.
      Purified NA/LE Mouse Anti-Human Integrin α5 chain (CD49e)
      Effects of anti-Integrin α5 chain (CD49e) antibodies on K562 cell binding to fibronectin. Aliquots of K562 cell suspension, an erythroleukemia cell line expressing Integrin α5 chain (CD49e) receptor, were incubated in the presence of the designated concentrations of Purified NA/LE Mouse Anti-Human Integrin α5 chain (CD49e) (Cat. No. 555614, • ) or Purified Mouse Anti-Human CD49e (Cat. No. 555651, □), for 30 minutes at room temperature. The suspensions were then transferred to plastic microtiter wells precoated with 12 µg/mL of fibronectin and incubated for 45 minutes at 37°C. Unattached cells were removed by aspiration and washing with PBS, and the attached cells were semi-quantitated using toluidine blue staining. The resulting absorbance signals are expressed relative to the average signal obtained without antibody.
      商品详情
      Down Arrow Up Arrow


      BD Pharmingen™
      Integrin α5; Integrin alpha-5; ITGA5; ITA5; FNRA; VLA-5; VLA5A
      Human (QC Testing)
      Mouse RBF/ DnJ IgG1, κ
      Human α5β1 Protein
      Flow cytometry (Routinely Tested)
      1.0 mg/ml
      3678
      AB_395981
      No azide/low endotoxin: Aqueous buffered solution containing no preservative, 0.2µm sterile filtered. Endotoxin level is ≤0.01 EU/µg (≤0.001 ng/µg) of protein as determined by the LAL assay.
      RUO


      准备和存储

      Store undiluted at 4°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. This preparation contains no preservatives, thus it should be handled under aseptic conditions.
      显示较多信息 blue down arrow 显示较少信息 blue up arrow

      商品通知

      1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
      2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
      3. Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
      显示较多信息 blue down arrow 显示较少信息 blue up arrow
      555614 Rev. 12
      抗体详情
      Down Arrow Up Arrow
      IIA1

      The IIA1 monoclonal antibody specifically recognizes CD49e, a type I transmembrane glycoprotein which is also known as Integrin α5 Chain or VLA-5 α chain (VLA-5α).  Following translation, CD49e is cleaved into two disulfide-linked chains of 135 and 25 kDa that associate with β1 integrin (CD29) to form the VLA-5 (Integrin α5/β1) complex. The VLA-5 complex is a well-established fibronectin receptor that is expressed on many cell types, including fibroblasts, endothelial cells, epithelial cells, platelets, thymocytes, T cells, B cells, the myeloid cell line U937, K562 and some melanoma cell lines. This clone inhibits α5β1 binding of ligands. It is useful for studies of the expression and function of this integrin.

      555614 Rev. 12
      格式详情
      Down Arrow Up Arrow
      NA/LE
      NA/LE refers to the culture and purification methods and buffer used to produce purified antibodies with no azide and low endotoxin: Aqueous buffered solution containing no preservative, 0.2µm sterile filtered. Endotoxin level is ≤0.01 EU/µg (≤0.001 ng/µg) of protein as determined by the LAL assay.NA/LE are perfectly suited to be used in culture or in vivo (for nonhuman studies) for functional assays — blocking, neutralizing, activation or depletion — where the presence of azide may damage cells or exogenous endotoxin may signal or activate cells.
      NA/LE
      555614 Rev.12
      报价单和参考
      Down Arrow Up Arrow
      View product citations for antibody "555614" on CiteAb

      研发参考 (1)

      1. Tran Van Nhieu G, Isberg RR. The Yersinia pseudotuberculosis invasin protein and human fibronectin bind to mutually exclusive sites on the alpha 5 beta 1 integrin receptor. J Biol Chem. 1991; 266(36):24367-24375. (Biology). 查看参考
      555614 Rev. 12

      Please refer to Support Documents for Quality Certificates

       

      Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

       

      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

      For Research Use Only. Not for use in diagnostic or therapeutic procedures.