Purified Mouse Anti-Human GITR (CD357)
Flow cytometric analysis of GITR (CD357) expression on human peripheral blood mononuclear cells. Human peripheral blood mononuclear cells were cultured for 3 days in medium with Phytohemagglutinin-M (PHA-M Stimulated; 1.5% v/v). The cells were then stained with either Purified Mouse IgG2b, κ Isotype Control (Cat. No. 555740; dashed line histogram) or Purified Mouse Anti-Human GITR (CD357) antibody (Cat. No. 567769; solid line histogram) followed by PE Goat Anti-Mouse Ig (Multiple Adsorption) (Cat. No. 550589). The fluorescent histogram showing GITR (CD357) expression (or Ig Isotype staining) was derived from gated events with the forward and side light-scatter characteristics of intact leucocytes. Flow cytometry and data analysis was performed using a BD LSRFortessa™ X-20 Flow Cytometer System and FloJo™ software.
BD Pharmingen™ Purified Mouse Anti-Human GITR (CD357)
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