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Purified Mouse Anti-Human CD94
Purified Mouse Anti-Human CD94

Flow cytometric analysis of CD94 expression on human peripheral blood lymphocytes. Human whole blood was stained with either Purified Mouse Anti-Human CD94 (Cat. No. 555887; solid line histogram) or Purified Mouse IgG1, κ Isotype Control (Cat. No. 555746; dashed line histogram), followed by FITC Goat Anti-Mouse IgG/IgM (Cat. No. 555887). Erythrocytes were lysed with BD Pharm Lyse™ Lysing Buffer (Cat. No. 555899). Fluorescent histograms were derived from gated events with the side and forward light-scattering characteristics of viable lymphocytes. Flow cytometry was performed on a BD FACScan™ system.

Flow cytometric analysis of CD94 expression on human peripheral blood lymphocytes. Human whole blood was stained with either Purified Mouse Anti-Human CD94 (Cat. No. 555887; solid line histogram) or Purified Mouse IgG1, κ Isotype Control (Cat. No. 555746; dashed line histogram), followed by FITC Goat Anti-Mouse IgG/IgM (Cat. No. 555887). Erythrocytes were lysed with BD Pharm Lyse™ Lysing Buffer (Cat. No. 555899). Fluorescent histograms were derived from gated events with the side and forward light-scattering characteristics of viable lymphocytes. Flow cytometry was performed on a BD FACScan™ system.

商品详情
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BD Pharmingen™
KLRD1; Killer cell lectin-like receptor subfamily D member 1; KP43
Human (QC Testing)
Mouse BALB/c IgG1, κ
Human NK Cells
Flow cytometry (Routinely Tested), Immunohistochemistry-frozen (Tested During Development)
0.5 mg/ml
V NK82
3824
AB_396199
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO


商品通知

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. An isotype control should be used at the same concentration as the antibody of interest.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. Sodium azide is a reversible inhibitor of oxidative metabolism; therefore, antibody preparations containing this preservative agent must not be used in cell cultures nor injected into animals. Sodium azide may be removed by washing stained cells or plate-bound antibody or dialyzing soluble antibody in sodium azide-free buffer. Since endotoxin may also affect the results of functional studies, we recommend the NA/LE (No Azide/Low Endotoxin) antibody format, if available, for in vitro and in vivo use.
  5. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
555887 Rev. 6
抗体详情
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HP-3D9

The HP-3D9 monoclonal antibody specifically binds to CD94 which is also known as KP43. CD94 is expressed on the cell surface as a ~70 kDa, disulfide-linked, type II transmembrane glycoprotein dimer. It is encoded by KLRD1 (Killer cell lectin like receptor D1) which belongs to the C-type lectin superfamily. CD94 is expressed on natural killer (NK) cells, especially activated NK cells. It is also expressed on γ/δ TCR+ T lymphocytes, NK-T cells, and on some CD8+CD56+ α/β TCR+ cells. CD94 associates with various NKG2 receptors to form receptors for HLA class I molecules and plays a role in regulating cellular adhesion and activation. The HP-3D9 antibody can reportedly inhibit the cytolytic activity of activated NK cells.

555887 Rev. 6
格式详情
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Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
555887 Rev.6
报价单和参考
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研发参考 (3)

  1. Aramburu J, Balboa MA, Ramírez A, et al. A novel functional cell surface dimer (Kp43) expressed by natural killer cells and T cell receptor-gamma/delta+ T lymphocytes. I. Inhibition of the IL-2-dependent proliferation by anti-Kp43 monoclonal antibody. J Immunol. 1990; 144(8):3238-3247. (Biology). 查看参考
  2. Balboa MA, Balsinde J, Aramburu J, Mollinedo F, López-Botet M. Phospholipase D activation in human natural killer cells through the Kp43 and CD16 surface antigens takes place by different mechanisms. Involvement of the phospholipase D pathway in tumor necrosis factor alpha synthesis. J Exp Med. 1992; 176(1):9-17. (Biology). 查看参考
  3. Pérez-Villar JJ, Melero I, Rodríguez A, et al. Functional ambivalence of the Kp43 (CD94) NK cell-associated surface antigen. J Immunol. 1995; 154(11):5779-5788. (Biology). 查看参考
555887 Rev. 6

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Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.