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PE Rat Anti-Human Clec9A (CD370)
PE Rat Anti-Human Clec9A (CD370)

Multicolor flow cytometric analysis of human Clec9a expression on dendritic cell subsets from human peripheral blood mononuclear cells. Human peripheral blood mononuclear cells (PBMC) were stained with either PE Rat IgG2a, κ Isotype Control (Cat. No. 553930; Left Panel) or PE Rat Anti-Human Clec9A (CD370) antibody (Cat. No. 563488; Right Panel). The cells were also stained with FITC Mouse Anti-Human HLA-DR (Cat No. 555811), BD Horizon™ BV711 Mouse Anti-Human CD141 (Cat. No. 563155) and a Lineage cocktail comprised of BD Horizon™ V450-conjugated Mouse Anti-Human CD3 (Cat. No. 560366), Mouse Anti-Human CD14 (Cat. No. 560350), Mouse Anti-Human CD19 (Cat. No. 560354) and Mouse Anti-Human CD56 (Cat. No. 560361) antibodies. Two-color flow cytometric dot plots show the correlated expression patterns of Clec9A (CD370) (or Ig Isotype control staining) versus CD141 for Lineage-negative, HLA-DR-positive gated events with the forward and side light-scatter characteristics of viable leucocytes. Flow cytometric analysis was performed using a BD LSRFortessa™ Flow Cytometer System.

Multicolor flow cytometric analysis of human Clec9a expression on dendritic cell subsets from human peripheral blood mononuclear cells. Human peripheral blood mononuclear cells (PBMC) were stained with either PE Rat IgG2a, κ Isotype Control (Cat. No. 553930; Left Panel) or PE Rat Anti-Human Clec9A (CD370) antibody (Cat. No. 563488; Right Panel). The cells were also stained with FITC Mouse Anti-Human HLA-DR (Cat No. 555811), BD Horizon™ BV711 Mouse Anti-Human CD141 (Cat. No. 563155) and a Lineage cocktail comprised of BD Horizon™ V450-conjugated Mouse Anti-Human CD3 (Cat. No. 560366), Mouse Anti-Human CD14 (Cat. No. 560350), Mouse Anti-Human CD19 (Cat. No. 560354) and Mouse Anti-Human CD56 (Cat. No. 560361) antibodies. Two-color flow cytometric dot plots show the correlated expression patterns of Clec9A (CD370) (or Ig Isotype control staining) versus CD141 for Lineage-negative, HLA-DR-positive gated events with the forward and side light-scatter characteristics of viable leucocytes. Flow cytometric analysis was performed using a BD LSRFortessa™ Flow Cytometer System.

商品详情
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BD Pharmingen™
CD370; C-type lectin domain family 9 member A; CLEC9A; DNGR1
Human (QC Testing), Monkey (Reported)
Rat WI, also known as Wistar (outbred) IgG2a, κ
Human Clec9A Peptide
Flow cytometry (Routinely Tested)
0.2 mg/ml
AB_2738237
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO


准备和存储

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed.

商品通知

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. An isotype control should be used at the same concentration as the antibody of interest.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  5. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
563488 Rev. 2
抗体详情
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3A4/Clec9A

The 3A4/Clec9A monoclonal antibody specifically binds to human Clec9A (C-type lectin domain family member 9A). Clec9A is a type II transmembrane glycoprotein with a single extracellular C-type lectin domain. Clec9A is also known as CD370 and DNGR1 (Dendritic cell natural killer lectin group receptor 1). Clec9A is selectively expressed on CD141+ myeloid dendritic cells. Clec9A reportedly functions as an endocytic receptor for necrotic cells. It can mediate the cross-presentation of dead-cell associated antigens by dendritic cells in a Syk-dependent manner. The 3A4 antibody reportedly crossreacts with peripheral blood dendritic cells form Pigtail macaques (Macaca nemestrina).

563488 Rev. 2
格式详情
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PE
R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
altImg
PE
Yellow-Green 488 nm, 532 nm, 561 nm
496 nm, 566 nm
576 nm
563488 Rev.2
报价单和参考
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研发参考 (6)

  1. Caminschi L, Proietto AL, Ahmet F, et al. The dendritic cell subtype restricted C-type lectin Clec9A is a target for vaccine enhancement. Blood. 2008; 112(8):3264-3273. (Immunogen: Flow cytometry). 查看参考
  2. Clark HF, Gurney AL, Abaya E, et al. The secreted protein discovery initiative (SPDI), a large-scale effort to identify novel human secreted and transmembrane proteins: a bioinformatics assessment. Genome Res. 2003; 13(10):2265-2270. (Biology). 查看参考
  3. Huysamen C, Willment JA, Dennehy KM, Brown GD. CLEC9A is a novel activation C-type lectin-like receptor expressed on BDCA3+ dendritic cells and a subset of monocytes. J Biol Chem. 2008; 283(24):16693-16701. (Biology). 查看参考
  4. Jongbloed SL, Kassianos AJ, McDonald KJ, et al. Human CD141+ (BDCA-3)+ dendritic cells (DCs) represent a unique myeloid DC subset that cross-presents necrotic cell antigens. J Exp Med. 2010; 207(6):1247-1260. (Clone-specific: Flow cytometry). 查看参考
  5. Sancho D, Mourão-Sá D, Joffre OP, et al. Tumor therapy in mice via antigen targeting to a novel, DC-restricted C-type lectin. J Clin Invest. 2008; 118(6):2098-2110. (Biology). 查看参考
  6. Zhang JG, Czabotar PE, Policheni AN, et al. The dendritic cell receptor Clec9A binds damaged cells via exposed actin filaments. Immunity. 2012; 36(4):646-657. (Biology). 查看参考
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563488 Rev. 2

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For Research Use Only. Not for use in diagnostic or therapeutic procedures.