Skip to main content Skip to navigation
Hamburger Menu
Search icon
Location Selector Location Selector
China (Chinese)
登录/注册 UserName
Products

Link Arrow
解决方案

Link Arrow
Discover & Learn

Link Arrow
Resources & Tools

Link Arrow
Support

Link Arrow
Close Menu
Website Feedback
Alert Icon
565196_Viperin_Image.png

Flow cytometric analysis of Viperin expression on LPS-stimulated lymphocytes. Human peripheral blood mononuclear cells were cultured overnight either without (Unstimulated; Left Panel) or with (LPS-Stimulated; Right Panel) lipopolysaccharide (1 μg/ml). The cells were washed, and then fixed and permeabilized using the BD Cytofix/Cytoperm™ Fixation/Permeabilization Solution Kit (Cat. No. 554714). Cells were stained with PE Mouse IgG2a, κ Isotype Control (Cat. No.554648; dashed line histograms) or PE Mouse Anti-Viperin antibody (Cat. No. 565196; solid line histograms). Histograms showing Viperin (or Ig Isotype control staining) were derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometric analysis was performed using a BD LSRFortessa™ Cell Analyzer System.

565196_Viperin_Image.png Red-Cap-Blue-Label.jpg

Flow cytometric analysis of Viperin expression on LPS-stimulated lymphocytes. Human peripheral blood mononuclear cells were cultured overnight either without (Unstimulated; Left Panel) or with (LPS-Stimulated; Right Panel) lipopolysaccharide (1 μg/ml). The cells were washed, and then fixed and permeabilized using the BD Cytofix/Cytoperm™ Fixation/Permeabilization Solution Kit (Cat. No. 554714). Cells were stained with PE Mouse IgG2a, κ Isotype Control (Cat. No.554648; dashed line histograms) or PE Mouse Anti-Viperin antibody (Cat. No. 565196; solid line histograms). Histograms showing Viperin (or Ig Isotype control staining) were derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometric analysis was performed using a BD LSRFortessa™ Cell Analyzer System.

商品详情
Down Arrow Up Arrow


BD Pharmingen™
Rsad2; Vig1; cig5
Human (QC Testing), Mouse (Tested in Development), Rat (Predicted)
Mouse IgG2a, κ
Mouse Viperin Recombinant Protein
Intracellular staining (flow cytometry) (Routinely Tested)
0.2 mg/ml
AB_2739106
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO


准备和存储

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed.
显示较多信息 blue down arrow 显示较少信息 blue up arrow

商品通知

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
  5. An isotype control should be used at the same concentration as the antibody of interest.
  6. Species cross-reactivity detected in product development may not have been confirmed on every format and/or application.
显示较多信息 blue down arrow 显示较少信息 blue up arrow
565196 Rev. 1
抗体详情
Down Arrow Up Arrow
MaP.VIP

The MaP.VIP monoclonal antibody specifically binds to Virus inhibitory protein, endoplasmic reticulum-associated, interferon-inducible (Viperin), which is also known as Radical S-adenosyl methionine domain-containing protein 2 (Rsad2). Viperin is a 43 kDa protein that is localized to the endoplasmic reticulum and lipid droplets. It functions in protective responses to a number of different DNA and RNA viruses. Viperin expression is induced in cells by different agents such as, Type I interferons, Interferon-γ (IFN-γ), DNA and RNA viral proteins, Polyinosinic:polycytidylic acid, and lipopolysaccharide.  Various cell types can be induced to express Viperin including T and B lymphocytes, macrophages, granulocytes, dendritic cells, fibroblasts, endothelial cells, and epithelial cells.

565196 Rev. 1
格式详情
Down Arrow Up Arrow
PE
R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
altImg
PE
496 nm, 566 nm
576 nm
565196 Rev.1
报价单和参考
Down Arrow Up Arrow
View product citations for antibody "565196" on CiteAb

研发参考 (9)

  1. Hinson ER, Cresswell P. The N-terminal amphipathic alpha-helix of viperin mediates localization to the cytosolic face of the endoplasmic reticulum and inhibits protein secretion. J Biol Chem. 2009; 284(7):4705-4712. (Clone-specific: Electron microscopy, Fluorescence microscopy, Immunocytochemistry (cytospins), Immunofluorescence, Western blot). 查看参考
  2. Hinson ER, Cresswell P. The antiviral protein, viperin, localizes to lipid droplets via its N-terminal amphipathic alpha-helix. Proc Natl Acad Sci U S A. 2009; 106(48):20452-20457. (Clone-specific: Flow cytometry, Fluorescence microscopy, Immunofluorescence, Western blot). 查看参考
  3. Hinson ER, Joshi NS, Chen JH, et al. Viperin is highly induced in neutrophils and macrophages during acute and chronic lymphocytic choriomeningitis virus infection. J Immunol. 2010; 184(10):5723-5731. (Clone-specific: Electron microscopy, Flow cytometry, Immunocytochemistry (cytospins)). 查看参考
  4. Mattijssen S, Pruijn GJ. Viperin, a key player in the antiviral response. Microbes Infect. 2012; 14(5):419-426. (Biology). 查看参考
  5. Qiu LQ, Cresswell P, Chin KC. Viperin is required for optimal Th2 responses and T-cell receptor-mediated activation of NF-kappaB and AP-1. Blood. 2009; 113(15):3520-3529. (Biology). 查看参考
  6. Seo JY, Cresswell P. Viperin regulates cellular lipid metabolism during human cytomegalovirus infection. PLoS ONE. 2013; 9(8):e1003497. (Clone-specific: Fluorescence microscopy, Immunofluorescence, Western blot). 查看参考
  7. Seo JY, Yaneva R, Hinson ER, Cresswell P. Human cytomegalovirus directly induces the antiviral protein viperin to enhance infectivity. Science. 2011; 332(6033):1093-1097. (Clone-specific: Electron microscopy, Fluorescence microscopy, Immunocytochemistry (cytospins), Immunofluorescence, Immunoprecipitation). 查看参考
  8. Szretter KJ, Brien JD, Thackray LB, Virgin HW, Cresswell P, Diamond MS. The interferon-inducible gene viperin restricts West Nile virus pathogenesis. J Virol. 2011; 85(22):11557-11566. (Clone-specific: Fluorescence microscopy, Immunofluorescence). 查看参考
  9. Wang X, Hinson ER, Cresswell P. The interferon-inducible protein viperin inhibits influenza virus release by perturbing lipid rafts. Cell. 2007; 2(2):96-105. (Immunogen: Fluorescence microscopy, Immunofluorescence). 查看参考
查看所有文件 (9) 查看更少内容
565196 Rev. 1

Please refer to Support Documents for Quality Certificates

 

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

 

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.