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PE Mouse anti-PLC-γ2 (pY759)
PE Mouse anti-PLC-γ2 (pY759)

Analysis of PLC-γ2 (pY759) in human Burkitt's lymphoma.  Ramos cells were either stimulated (open histogram) by cross-linking of surface IgM with purified Goat anti-Human IgM (SouthernBiotech) at 37°C for 3 minutes or unstimulated (solid histogram).  The cells were fixed with pre-warmed BD Phosflow™ Fix Buffer I (Cat. No. 557870) for 10 minutes at 37ºC, then permeabilized with BD Phosflow™ Perm Buffer III (Cat. No. 558050) on ice for at least 10 minutes, and then stained with PE Mouse anti-PLC-γ2 (pY759, Cat. No. 558490).  Flow cytometry was performed on a BD FACSCalibur™ flow cytometry system.

Analysis of PLC-γ2 (pY759) in human Burkitt's lymphoma.  Ramos cells were either stimulated (open histogram) by cross-linking of surface IgM with purified Goat anti-Human IgM (SouthernBiotech) at 37°C for 3 minutes or unstimulated (solid histogram).  The cells were fixed with pre-warmed BD Phosflow™ Fix Buffer I (Cat. No. 557870) for 10 minutes at 37ºC, then permeabilized with BD Phosflow™ Perm Buffer III (Cat. No. 558050) on ice for at least 10 minutes, and then stained with PE Mouse anti-PLC-γ2 (pY759, Cat. No. 558490).  Flow cytometry was performed on a BD FACSCalibur™ flow cytometry system.

商品详情
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BD Phosflow™
Human (QC Testing), Mouse, Rat (Predicted)
Mouse IgG1, κ
Phosphorylated Human PLC-γ2 Peptide
Intracellular staining (flow cytometry) (Routinely Tested)
20 µl
AB_647226
Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
RUO


准备和存储

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed.

商品通知

  1. This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
  2. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
  5. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
558490 Rev. 2
抗体详情
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K86-689.37

The Phospholipase C (PLC) isozymes hydrolyze phosphatidyl inositol biphosphate to inositol triphosphate and diacylglycerol.  The former causes release of calcium from the endoplasmic reticulum, while the latter is an activator of Protein Kinase C.  Within the PLC family, PLC-γ is the only member that contains SH2 and SH3 domains.  These domains enable it to interact with protein tyrosine kinases and become enzymatically activated via phosphorylation.  It exists as two isoforms: PLC-γ1, which is ubiquitously expressed, and PLC-γ2, which is found primarily in the hematopoietic system.  PLC-γ2-null mice exhibit impaired B lymphocyte maturation and defects in Fc receptor functions.  Phosphorylation of PLC-γ2 at tyrosines 753 and 759 (Y759) is required for activation of PLC-γ2 enzyme activity.  PLC-γ2 phosphorylation at Y759 can be induced by stimulation of the B cell receptor in Ramos cells, the collagen receptor in platelets, and the T cell receptor in Jurkat cells, and it occurs downstream of Btk and BLNK in the signaling cascade of activated B cells.  

The K86-689.37.73 antibody recognizes PLC-γ2 phosphorylated at Y759 in the SH2-SH3 linker region.

558490 Rev. 2
格式详情
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PE
R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
altImg
PE
Yellow-Green 488 nm, 532 nm, 561 nm
496 nm, 566 nm
576 nm
558490 Rev.2
报价单和参考
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研发参考 (4)

  1. Kim YJ, Sekiya F, Poulin B, Bae YS, Rhee SG. Mechanism of B-cell receptor-induced phosphorylation and activation of phospholipase C-γ2. Mol Cell Biol. 2004; 24(22):9986-9999. (Biology).
  2. Ozdener F, Dangelmaier C, Ashby B, Kunapuli SP, Daniel JL. Activation of phospholipase Cγ2 by tyrosine phosphorylation.. Mol Pharmacol. 2002; 62(3):672-679. (Biology).
  3. Wang D, Feng J, Wen R, et al. Phospholipase Cγ2 is essential in the functions of B cell and several Fc receptors. Immunity. 2000; 13:25-35. (Biology).
  4. Wen R, Jou S-T, Chen Y, Hoffmeyer A, Wang D. Phospholipase Cγ2 is essential for specific functions of FcεR and FcγR . J Immunol. 2002; 169:6743-6752. (Biology).
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558490 Rev. 2

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Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.