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PE Mouse Anti-Mouse LAP
563143Image1.png

Multicolor flow cytometric analysis of LAP expression on activated mouse CD4+ T cells.  Leucocytes from a BALB/c mouse spleen were prepared. These cells were activated in culture (2 days) with plate-bound Purified NA/LE Hamster Anti-Mouse CD3 (Cat. No. 553057) and Purified NA/LE Hamster Anti-Mouse CD28 (Cat. No. 553294) antibodies in the presence of Purified Natural Transforming Growth Factor-b (TGF-b; Cat. No. 356040/356039/354039). The cells were harvested and stained with BD Horizon™ V450 Rat Anti-Mouse CD4 (Cat. No. 560468) and either PE Mouse IgG1, κ Isotype Control (Cat. No. 554680; Left Panel) or PE Mouse Anti-Mouse LAP antibody (Cat. No. 563143; Right Panel). Two-color flow cytometric dot plots show the correlated expression patterns of CD4 versus LAP (or Ig Isotype control staining) for gated events with the forward and side light-scatter characteristics of activated lymphocytes. Flow cytometry was performed using a BD LSRFortessa™ Cell Analyzer System.

mLAP_PE_Final_Final.png

Multicolor flow cytometric analysis of LAP expression on activated mouse CD4+ T cells.  Leucocytes from a BALB/c mouse spleen were prepared. These cells were activated in culture (2 days) with plate-bound Purified NA/LE Hamster Anti-Mouse CD3 (Cat. No. 553057) and Purified NA/LE Hamster Anti-Mouse CD28 (Cat. No. 553294) antibodies in the presence of Purified Natural Transforming Growth Factor-b (TGF-b; Cat. No. 356040/356039/354039). The cells were harvested and stained with BD Horizon™ V450 Rat Anti-Mouse CD4 (Cat. No. 560468) and either PE Mouse IgG1, κ Isotype Control (Cat. No. 554680; Left Panel) or PE Mouse Anti-Mouse LAP antibody (Cat. No. 563143; Right Panel). Two-color flow cytometric dot plots show the correlated expression patterns of CD4 versus LAP (or Ig Isotype control staining) for gated events with the forward and side light-scatter characteristics of activated lymphocytes. Flow cytometry was performed using a BD LSRFortessa™ Cell Analyzer System.

563143Image1.png mLAP_PE_Final_Final.png

Multicolor flow cytometric analysis of LAP expression on activated mouse CD4+ T cells.  Leucocytes from a BALB/c mouse spleen were prepared. These cells were activated in culture (2 days) with plate-bound Purified NA/LE Hamster Anti-Mouse CD3 (Cat. No. 553057) and Purified NA/LE Hamster Anti-Mouse CD28 (Cat. No. 553294) antibodies in the presence of Purified Natural Transforming Growth Factor-b (TGF-b; Cat. No. 356040/356039/354039). The cells were harvested and stained with BD Horizon™ V450 Rat Anti-Mouse CD4 (Cat. No. 560468) and either PE Mouse IgG1, κ Isotype Control (Cat. No. 554680; Left Panel) or PE Mouse Anti-Mouse LAP antibody (Cat. No. 563143; Right Panel). Two-color flow cytometric dot plots show the correlated expression patterns of CD4 versus LAP (or Ig Isotype control staining) for gated events with the forward and side light-scatter characteristics of activated lymphocytes. Flow cytometry was performed using a BD LSRFortessa™ Cell Analyzer System.

Multicolor flow cytometric analysis of LAP expression on activated mouse CD4+ T cells.  Leucocytes from a BALB/c mouse spleen were prepared. These cells were activated in culture (2 days) with plate-bound Purified NA/LE Hamster Anti-Mouse CD3 (Cat. No. 553057) and Purified NA/LE Hamster Anti-Mouse CD28 (Cat. No. 553294) antibodies in the presence of Purified Natural Transforming Growth Factor-b (TGF-b; Cat. No. 356040/356039/354039). The cells were harvested and stained with BD Horizon™ V450 Rat Anti-Mouse CD4 (Cat. No. 560468) and either PE Mouse IgG1, κ Isotype Control (Cat. No. 554680; Left Panel) or PE Mouse Anti-Mouse LAP antibody (Cat. No. 563143; Right Panel). Two-color flow cytometric dot plots show the correlated expression patterns of CD4 versus LAP (or Ig Isotype control staining) for gated events with the forward and side light-scatter characteristics of activated lymphocytes. Flow cytometry was performed using a BD LSRFortessa™ Cell Analyzer System.

商品详情
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BD Pharmingen™
Latency-associated peptide; TGFB1; TGFbeta; Transforming growth factor beta
Mouse (QC Testing)
Mouse IgG1, κ
Mouse Tgfb1-transduced Cell Line
Flow cytometry (Routinely Tested)
0.2 mg/ml
AB_2738027
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO


准备和存储

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed.
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商品通知

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. An isotype control should be used at the same concentration as the antibody of interest.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
  5. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
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563143 Rev. 1
抗体详情
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TW7-16B4

The TW7-16B4 monoclonal antibody specifically binds to Latency-Associated Peptide (LAP), a component of the dimeric Transforming Growth Factor-beta 1 (TGF-β1) propeptide encoded by Tgfb1. Prior to secretion, the dimeric LAP-TGF-β1 propeptide is cleaved resulting in a biologically inactive form of dimeric TGF-β1 that is noncovalently associated with dimeric LAP (latent TGF-β1). This complex may be expressed on the surface of TGF-β1-producing cells or be further processed by proteolytic removal of LAP to release the biologically active mature form of the soluble TGF-β1 homodimer. Platelets contain TGF-β1 and most nucleated cells, including tumor cells and cells that comprise the innate and adaptive immune system can produce TGF-β1. TGF-β1 is a potent multifunctional cytokine that regulates numerous processes including development, hematopoiesis, tissue remodeling, wound repair, and immunity as well as cancer and autoimmune diseases.

563143 Rev. 1
格式详情
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PE
R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
altImg
PE
496 nm, 566 nm
576 nm
563143 Rev.1
报价单和参考
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View product citations for antibody "563143" on CiteAb

研发参考 (4)

  1. Oida T, Weiner HL. Overexpression of TGF-β1 gene induces cell surface localized glucose-regulated protein 78-associated latency-associated peptide/TGF-β. J Immunol. 2010; 185(6):3529-3535. (Biology). 查看参考
  2. Oida T, Weiner HL. TGF-β induces surface LAP expression on murine CD4 T cells independent of Foxp3 induction. PLoS ONE. 2010; 5(11):e15523. (Immunogen: Flow cytometry, Immunoprecipitation, Western blot). 查看参考
  3. Oida T, Zhang X, Goto M, et al. CD4+CD25- T cells that express latency-associated peptide on the surface suppress CD4+CD45RBhigh-induced colitis by a TGF-beta-dependent mechanism. J Immunol. 2003; 170(5):2516-2522. (Biology). 查看参考
  4. Wilkinson KA, Martin TD, Reba SM, et al. Latency-Associated Peptide of Transforming Growth Factor β enhances mycobacteriocidal immunity in the lung during Mycobacterium bovis BCG infection in C57BL/6 mice. Infect Immun. 2000; 68(11):6505-6508. (Biology). 查看参考
查看所有文件 (4) 查看更少内容
563143 Rev. 1

Please refer to Support Documents for Quality Certificates

 

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

 

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.