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PE Mouse Anti-Mouse CD157
商品详情
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BD Pharmingen™
BP-3 Alloantigen
Mouse (QC Testing)
Mouse Mus spretus, also known as STF Mus spretus IgG2b, κ
Mouse pre-B cell line
Flow cytometry (Routinely Tested)
0.2 mg/ml
AB_394472
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO


准备和存储

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
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推荐的实验流程

For optimal staining of peripheral lymphoid tissue, we recommend the use of Mouse BD Fc Block™ (anti-mouse CD16/CD32 mAb 2.4G2, Cat. No. 553141/553142).

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商品通知

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  3. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
  4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
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552814 Rev. 1
抗体详情
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BP-3

The BP-3 antibody reacts with CD157, also known as the BP-3 alloantigen or Bone marrow STromal cell antigen-1 (BST-1), found in a variety of common laboratory strains of mice (AKR, BALB/c, CBA/N, C3H/HeJ, C57BL/6, DBA/2, NZB, C.B-17, SJL and Swiss, but not A/J). CD157 is a differentially glycosylated GPI-linked cell-surface glycoprotein with a core protein of 32 kDa and differing patterns of glycosylation in different types of cells. It is a member, along with CD38, of a family of genetically-related ectoenzymes. The BP-3 alloantigen is expressed on the B lineage from the earliest detectable progenitors to circulating immature B lymphocytes, on immature thymocytes up to the   expression of the T-cell receptor, on circulating neutrophils, peritoneal macrophages, on subpopulations of stromal cells in peripheral lymphoid organs (but not in bone marrow or thymus), on intestinal epithelial cells, and in the  lumen of kidney-collecting tubules. The distribution of CD157 antigen on leukocytes and stromal cells of lymphoid organs suggests that it may be involved in leukocyte-stroma interactions which support the development, migration, and/or responses of leukocyte populations.

552814 Rev. 1
格式详情
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PE
R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
altImg
PE
496 nm, 566 nm
576 nm
552814 Rev.1
报价单和参考
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View product citations for antibody "552814" on CiteAb

研发参考 (6)

  1. Dong C, Wang J, Neame P, Cooper MD. The murine BP-3 gene encodes a relative of the CD38/NAD glycohydrolase family. Int Immunol. 1994; 6(9):1353-1360. (Biology). 查看参考
  2. Dong C, Willerford D, Alt FW, Cooper MD. Genomic organization and chromosomal localization of the mouse Bp3 gene, a member of the CD38/ADP-ribosyl cyclase family. Immunogenetics. 1996; 45(1):35-43. (Biology). 查看参考
  3. Itoh M, Ishihara K, Hiroi T, et al. Deletion of bone marrow stromal cell antigen-1 (CD157) gene impaired systemic thymus independent-2 antigen-induced IgG3 and mucosal TD antigen-elicited IgA responses. J Immunol. 1998; 161(8):3974-3983. (Biology). 查看参考
  4. McNagny KM, Bucy RP, Cooper MD. Reticular cells in peripheral lymphoid tissues express the phosphatidylinositol-linked BP-3 antigen. Eur J Immunol. 1991; 21(2):509-515. (Biology). 查看参考
  5. McNagny KM, Cazenave PA, Cooper MD. BP-3 alloantigen. A cell surface glycoprotein that marks early B lineage cells and mature myeloid lineage cells in mice. J Immunol. 1988; 141(8):2551-2556. (Immunogen). 查看参考
  6. Ngo VN, Korner H, Gunn MD, et al. Lymphotoxin alpha/beta and tumor necrosis factor are required for stromal cell expression of homing chemokines in B and T cell areas of the spleen. J Exp Med. 1999; 189(2):403-412. (Biology). 查看参考
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552814 Rev. 1

Please refer to Support Documents for Quality Certificates

 

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

 

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.