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      PE Mouse Anti-Human Osteocalcin
      PE Mouse Anti-Human Osteocalcin
      Flow cytometric analysis of osteocaclin expression in BGLAP-transfected HeLa cells.  Untransfected (dashed line histogram) and human BGLAP-transfected (solid line histogram) HeLa cells were fixed with BD Cytofix™ Fixation Buffer (Cat. No. 554655) and permeabilized with BD Phosflow™ Perm Buffer III (Cat. No.558050). The cells were then washed and stained with PE Mouse Anti-Human Osteocalcin antibody (Cat. No. 564146) using BD Biosciences Protocol for Intracellular Immunofluorescent Staining. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells. Flow cytometric analysis was performed using a BD FACSCanto™ II Flow Cytometer System.
      PE Mouse Anti-Human Osteocalcin
      Western blot analysis of osteocalcin expression by differentiated human Adipose Derived Stem Cells (ADSC).  Cell lysates from undifferentiated (Left Blot) and differentiated (Right Blot) were electrophoresed (SDS-PAGE), transferred to membranes and then blotted using purified Mouse Anti-Osteocalcin antibody, Clone R14-707. Osteocaclin is identified as a band of ~11 kDa in the differentiated ADSC cell lysate when blotted with the R14-707 antibody at concentrations of 5 (lane 5), 2.5 (lane 6), 1.25 (lane 7), and 0.63 (lane 8) μg/mL.
      PE Mouse Anti-Human Osteocalcin PE Mouse Anti-Human Osteocalcin
      Flow cytometric analysis of osteocaclin expression in BGLAP-transfected HeLa cells.  Untransfected (dashed line histogram) and human BGLAP-transfected (solid line histogram) HeLa cells were fixed with BD Cytofix™ Fixation Buffer (Cat. No. 554655) and permeabilized with BD Phosflow™ Perm Buffer III (Cat. No.558050). The cells were then washed and stained with PE Mouse Anti-Human Osteocalcin antibody (Cat. No. 564146) using BD Biosciences Protocol for Intracellular Immunofluorescent Staining. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells. Flow cytometric analysis was performed using a BD FACSCanto™ II Flow Cytometer System.
      Western blot analysis of osteocalcin expression by differentiated human Adipose Derived Stem Cells (ADSC).  Cell lysates from undifferentiated (Left Blot) and differentiated (Right Blot) were electrophoresed (SDS-PAGE), transferred to membranes and then blotted using purified Mouse Anti-Osteocalcin antibody, Clone R14-707. Osteocaclin is identified as a band of ~11 kDa in the differentiated ADSC cell lysate when blotted with the R14-707 antibody at concentrations of 5 (lane 5), 2.5 (lane 6), 1.25 (lane 7), and 0.63 (lane 8) μg/mL.
      商品详情
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      BD Pharmingen™
      BGLAP; BGP; Bone Gla protein; OC; OSTCN; PMF1
      Human (QC Testing)
      Mouse IgG1, κ
      Human Osteocalcin Recombinant Protein
      Intracellular staining (flow cytometry) (Routinely Tested)
      5 µl
      AB_2738620
      Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
      RUO


      准备和存储

      Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed.
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      商品通知

      1. This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
      2. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
      3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
      4. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
      5. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
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      564146 Rev. 1
      抗体详情
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      R14-707

      The R14-707 monoclonal antibody specifically recognizes human Osteocalcin. Osteocalcin (OC/OCN/OSTCN) is encoded by the BGLAP gene and is also known as Bone gamma-carboxyglutamic acid-containing protein. Osteocalcin is an approximately ~11 kDa protein that is secreted by osteoblasts. Its carboxylated form binds hydroxyapatite and thus it is abundantly found in bone extracellular matrix. Its uncarboxylated form serves as a hormone that can positively regulate glucose homeostasis. Serum osteocalcin has also been used as a biomarker for bone formation and turnover.

      564146 Rev. 1
      格式详情
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      PE
      R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
      altImg
      PE
      Yellow-Green 488 nm, 532 nm, 561 nm
      496 nm, 566 nm
      576 nm
      564146 Rev.1
      报价单和参考
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      View product citations for antibody "564146" on CiteAb

      研发参考 (5)

      1. Delmas PD, Stenner D, Wahner HW, Mann KG, Riggs BL. Increase in serum bone gamma-carboxyglutamic acid protein with aging in women: implications for the mechanism of age-related bone loss. J Clin Invest. 1983; 71(5):1316-1321. (Biology). 查看参考
      2. Kapustin AN, Shanahan CM. Osteocalcin: A Novel Vascular Metabolic and Osteoinductive Factor. Arterioscler Thromb Vasc Biol. 2011; 31(10):2169-2171. (Biology). 查看参考
      3. Levy RJ, Gundberg C, Scheinman R. The identification of the vitamin K-dependent bone protein osteocalcin as one of the gamma-carboxyglutamic acid containing proteins present in calcified atherosclerotic plaque and mineralized heart valves. Atherosclerosis. 1983; 46(1):49-56. (Biology). 查看参考
      4. Rossini M, Gatti D, Viapiana O, et al. Short-term effects on bone turnover markers of a single high dose of oral vitamin D3. J Clin Endocrinol Metab. 2012; 97(4):E622-E626. (Biology). 查看参考
      5. Schwetz V, Pieber T, Obermayer-Pietsch B. The endocrine role of the skeleton: background and clinical evidence. Eur J Endocrinol. 2012; 166(6):959-967. (Biology). 查看参考
      查看所有文件 (5) 查看更少内容
      564146 Rev. 1

      Please refer to Support Documents for Quality Certificates

       

      Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

       

      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

      For Research Use Only. Not for use in diagnostic or therapeutic procedures.