Flow cytometric analysis of HLA-DR expression on human peripheral blood lymphocytes. Whole blood was stained with either PE Mouse Anti-Human HLA-DR (Cat. No. 555561; solid line histogram) or PE Mouse IgG2b κ Isotype Control (Cat. No. 5557423 dashed line histogram). Erythrocytes were lysed with BD Pharm Lyse™ Lysing Buffer (Cat. No. 555899). Fluorescent histograms were derived from gated events with the side and forward light-scattering characteristics of viable lymphocytes. Flow cytometry was performed on a BD FACScan™ system
Flow cytometric analysis of HLA-DR expression on human peripheral blood lymphocytes. Whole blood was stained with either PE Mouse Anti-Human HLA-DR (Cat. No. 555561; solid line histogram) or PE Mouse IgG2b κ Isotype Control (Cat. No. 5557423 dashed line histogram). Erythrocytes were lysed with BD Pharm Lyse™ Lysing Buffer (Cat. No. 555899). Fluorescent histograms were derived from gated events with the side and forward light-scattering characteristics of viable lymphocytes. Flow cytometry was performed on a BD FACScan™ system
商品详情
BD Pharmingen™
Human (QC Testing), Rhesus, Cynomolgus, Baboon, Dog, Rabbit (Tested in Development)
Mouse IgG2b, κ
Human PBMC and SL6866 Cell Line
Flow cytometry (Routinely Tested)
20 µl
AB_395943
Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
RUO
准备和存储
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed.
商品通知
- This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- An isotype control should be used at the same concentration as the antibody of interest.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
- Species cross-reactivity detected in product development may not have been confirmed on every format and/or application.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
配套商品
Stain Buffer (FBS) RUO
尺寸
500 mL
品类编号
554656
Stain Buffer (BSA) RUO
尺寸
500 mL
品类编号
554657
Lysing Solution 10X Concentrate CFDA_IVD
尺寸
100 mL
品类编号
349202
Lysing Buffer RUO
尺寸
100 mL
品类编号
555899
PE Mouse IgG2b κ Isotype Control RUO
尺寸
100 Tests
品类编号
555743
555561 Rev. 10
抗体详情
TU36
The TU36 monoclonal antibody specifically binds to a monomorphic epitope on major histocompatibility complex (MHC) Class II HLA-DR antigens expressed on B cells, activated T cells, and antigen presenting cells. The antibody detects the MHC Class II αβ complex and not the isolated α or β chains. This antibody fixes complement.
555561 Rev. 10
格式详情
PE
R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
PE
Yellow-Green 488 nm, 532 nm, 561 nm
496 nm, 566 nm
576 nm
555561 Rev.10
报价单和参考
研发参考 (3)
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Pawelec G, Ziegler A, Wernet P. Dissection of human allostimulatory determinants with cloned T cells: stimulation inhibition by monoclonal antibodies TU22, 34, 35, 36, 37, 39, 43, and 58 against distinct human MHC class II molecules. Hum Immunol. 1985; 12(3):165-176. (Biology). 查看参考
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Ziegler A, Heinig J, Muller C, et al. Analysis by sequential immunoprecipitations of the specificities of the monoclonal antibodies TU22,34,35,36,37,39,43,58 and YD1/63.HLK directed against human HLA class II antigens. Immunobiology. 1986; 171(1-2):77-92. (Biology). 查看参考
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Ziegler A, Uchańska-Ziegler B, Zeuthen J, Wernet P. HLA antigen expression at the single cell level on a K562 X B cell hybrid: an analysis with monoclonal antibodies using bacterial binding assays.. Somatic Cell Genet. 1982; 8(6):775-89. (Immunogen). 查看参考
555561 Rev. 10
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.
