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      PE Mouse Anti-Human CD34

      BD Pharmingen™ PE Mouse Anti-Human CD34

      克隆 QBEND/10.rMAb (also known as QB-END-10, QBEND10, QBEND/10, QBEnd10,)

      (RUO)
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      PE Mouse Anti-Human CD34
      Multicolor flow cytometric analysis of CD34 expression on human peripheral blood mononuclear cells. Human peripheral blood mononuclear cells (PBMC) were preincubated in the presence of Human BD Fc Block™ (Cat. No. 564219/564220) and stained with BD Horizon™ BV786 Mouse Anti-Human CD14 (Cat. No. 563698), APC Mouse Anti-Human CD133 (Cat. No. 566597) and either PE Mouse IgG1, κ Isotype Control (Cat No. 349043; Left Plot) or PE Mouse Anti-Human CD34 antibody (Cat No. 568294/568295; Right Plot). The pseudocolor density plot showing the correlated expression of CD34 (or Ig Isotype control staining) versus CD133 was derived from gated events with the forward and side-light scatter characteristics of viable (CD14-negative) lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa™ X-20 Flow Cytometer System and FlowJo™ software.
      PE Mouse Anti-Human CD34
      Multicolor flow cytometric analysis of CD34 expression on human peripheral blood mononuclear cells. Human peripheral blood mononuclear cells (PBMC) were preincubated in the presence of Human BD Fc Block™ (Cat. No. 564219/564220) and stained with BD Horizon™ BV786 Mouse Anti-Human CD14 (Cat. No. 563698), APC Mouse Anti-Human CD133 (Cat. No. 566597) and either PE Mouse IgG1, κ Isotype Control (Cat No. 349043; Left Plot) or PE Mouse Anti-Human CD34 antibody (Cat No. 568294/568295; Right Plot). The pseudocolor density plot showing the correlated expression of CD34 (or Ig Isotype control staining) versus CD133 was derived from gated events with the forward and side-light scatter characteristics of viable (CD14-negative) lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa™ X-20 Flow Cytometer System and FlowJo™ software.
      商品详情
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      BD Pharmingen™
      CD34; CD34 antigen
      Human (QC Testing)
      Mouse IgG1, λ2
      Human Placental Vesicles
      Flow cytometry (Routinely Tested)
      5 µl
      Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
      RUO


      准备和存储

      Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to the dye under optimum conditions and unconjugated antibody and free dye were removed.
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      推荐的实验流程

      BD® CompBeads can be used as surrogates to assess fluorescence spillover (compensation).  When fluorochrome conjugated antibodies are bound to BD® CompBeads, they have spectral properties very similar to cells.   However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls.  It is strongly recommended that when using a reagent for the first time, users compare the spillover on cell and BD® CompBeads to ensure that BD® CompBeads are appropriate for your specific cellular application.

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      商品通知

      1. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
      2. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
      3. This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
      4. An isotype control should be used at the same concentration as the antibody of interest.
      5. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
      6. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
      7. Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
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      568294 Rev. 2
      抗体详情
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      QBEND/10.rMAb

      The QBEND/10.rMab recombinant monoclonal antibody specifically recognizes human CD34, a 105-120 kDa single-chain type I transmembrane glycoprotein, also known as CD34 antigen. CD34 is expressed on immature hematopoietic progenitor cells and all hematopoietic colony-forming cells in the bone marrow and blood, including vascular endothelium cells and some tissue fibroblasts. Normal peripheral blood lymphocytes, monocytes, granulocytes, and platelets do not express CD34. CD34 density is highest on early hematopoietic progenitor cells and decreases as cells mature. The antigen is absent on fully differentiated hematopoietic cells. The cytoplasmic region of the CD34 antigen is a target for phosphorylation by activated protein kinase C suggesting CD34 may play a role in signal transduction. CD34 has also been named as a possible adhesion molecule by mediating the attachment of stem cells to the bone marrow extracellular matrix or directly to stromal cells. The QBEND/10.rMAb antibody recognizes an epitope on CD34 distinct from those recognized by clones My10, 563, 581, and 8G12.

      568294 Rev. 2
      格式详情
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      PE
      R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
      altImg
      PE
      Yellow-Green 488 nm, 532 nm, 561 nm
      496 nm, 566 nm
      576 nm
      568294 Rev.2
      报价单和参考
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      View product citations for antibody "568294" on CiteAb

      研发参考 (6)

      1. Orfao A, Matarraz S, Pérez-Andrés M, et al. Immunophenotypic dissection of normal hematopoiesis. J Immunol Methods. 2019; 475:112684. (Biology: Flow cytometry). 查看参考
      2. Radtke S, Görgens A, Kordelas L, et al. CD133 allows elaborated discrimination and quantification of haematopoietic progenitor subsets in human haematopoietic stem cell transplants.. Br J Haematol. 2015; 169(6):868-78. (Biology: Flow cytometry). 查看参考
      3. Ramani P, Bradley NJ, Fletcher CD. QBEND/10, a new monoclonal antibody to endothelium: assessment of its diagnostic utility in paraffin sections.. Histopathology. 1990; 17(3):237-42. (Immunogen: Immunohistochemistry). 查看参考
      4. Sankey EA, More L, Dhillon AP. QBEnd/10: a new immunostain for the routine diagnosis of Kaposi's sarcoma.. J Pathol. 1990; 161(3):267-71. (Clone-specific: Immunohistochemistry). 查看参考
      5. Sutherland DR, Keating A. The CD34 antigen: structure, biology, and potential clinical applications.. J Hematother. 1992; 1(2):115-29. (Clone-specific: Cell separation, Flow cytometry). 查看参考
      6. Sutherland DR, Stewart AK, Keating A. CD34 antigen: molecular features and potential clinical applications.. Stem Cells. 1993; 11 Suppl 3:50-7. (Clone-specific: Cell separation, Flow cytometry). 查看参考
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      568294 Rev. 2

      Please refer to Support Documents for Quality Certificates


      Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

      For Research Use Only. Not for use in diagnostic or therapeutic procedures.