PE Mouse Anti-Human CD138

BD Pharmingen™ PE Mouse Anti-Human CD138

Name: PE Mouse Anti-Human CD138
Brand: PE Mouse Anti-Human CD138
SKU: 550805

克隆 DL-101 (RUO)

Profile of U266 (myeloma) cell line analyzed by flow cytometry

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实验方案

科学资源

商品详情

Brand: BD Pharmingen™

Alternative Name: Syndecan-1

Reactivity: Human (QC Testing)

Isotype: Mouse IgG1, κ

Application: Flow cytometry (Routinely Tested)

Vol. Per Test: 20 µl

RRID: AB_393890

Storage Buffer: Aqueous buffered solution containing BSA and ≤0.09% sodium azide.

Regulatory Status: RUO

准备和存储

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

商品通知

This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
Since applications vary, each investigator should titrate the reagent to obtain optimal results.
Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
Source of all serum proteins is from USDA inspected abattoirs located in the United States.

配套商品

PE Mouse IgG1, κ Isotype Control RUO

尺寸 100 Tests 品类编号 555749

550805 Rev. 5

抗体详情

DL-101

Reacts with CD138 (Syndecan-1), an 85-92 kDa single chain transmembrane protein, expressed on multiple myeloma-derived cell lines and malignant plasma cell populations. It is also expressed on pre-B cells, immature B cells and plasma cells, but not on mature circulating B-lymphocytes. Syndecan-1 is a member of the family of transmembrane heparan sulfate proteoglycans. It is also expressed on some non-hematopoietic cells, including embryonic mesenchymal cells, vascular smooth muscle cells, endothelial and neural cells. CD138 binds to many extracellular matrix proteins through its heparan sulfate side-chains, like fibronectin, collagen types I, III and V, tenascin, thrombospondin, and antithrombin III. It is considered an extracellular matrix receptor that may serve as a co-receptor for fibroblast growth factor and related molecules. Monoclonal antibody DL-101 does not block the binding of clone B-B4 (another anti-syndecan-1 antibody) or Mi15 by flow cytometric analysis.

550805 Rev. 5

格式详情

R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.

格式: PE

激发源: Yellow-Green 488 nm, 532 nm, 561 nm

最多激发源: 496 nm, 566 nm

最多发射源: 576 nm

550805 Rev.5

报价单和参考

View product citations for antibody "550805" on CiteAb

研发参考 (4)

Barclay NA, Brown MH, Birkeland ML, et al, ed. The Leukocyte Antigen FactsBook. San Diego, CA: Academic Press; 1997.
Costes V, Magen V, Legouffe E, et al. The Mi15 monoclonal antibody (anti-syndecan-1) is a reliable marker for quantifying plasma cells in paraffin-embedded bone marrow biopsy specimens. Hum Pathol. 1999; 30(12):1405-1411. (Clone-specific: Flow cytometry). 查看参考
Gattei V, Godeas C, Degan M, Rossi FM, Aldinucci D, Pinto A. Characterization of anti-CD138 monoclonal antibodies as tools for investigating the molecular polymorphism of syndecan-1 in human lymphoma cells. Br J Haematol. 1999; 104(1):152-162. (Clone-specific: Flow cytometry). 查看参考
Kishimoto T. Tadamitsu Kishimoto .. et al., ed. Leucocyte typing VI : white cell differentiation antigens : proceedings of the sixth international workshop and conference held in Kobe, Japan, 10-14 November 1996. New York: Garland Pub.; 1997.

查看所有文件 (4)

550805 Rev. 5

Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.