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PE Hamster Anti-Mouse Vβ 8.3 T-Cell Receptor
PE Hamster Anti-Mouse Vβ 8.3 T-Cell Receptor

Two-color analysis of the expression of Vβ 8.3 TCR on peripheral lymphocytes. C57BL/6 lymph node cells were incubated simultaneously with PE-conjugated 1B3.3, FITC-conjugated anti-mouse CD4 RM4-5 (Cat. No. 553046/553047), and FITC-conjugated anti-mouse CD8a 53-6.7 (Cat. No. 553030/553031) monoclonal antibodies. Flow cytometry was performed on a BD FACScan™ flow cytometry system.

Two-color analysis of the expression of Vβ 8.3 TCR on peripheral lymphocytes. C57BL/6 lymph node cells were incubated simultaneously with PE-conjugated 1B3.3, FITC-conjugated anti-mouse CD4 RM4-5 (Cat. No. 553046/553047), and FITC-conjugated anti-mouse CD8a 53-6.7 (Cat. No. 553030/553031) monoclonal antibodies. Flow cytometry was performed on a BD FACScan™ flow cytometry system.

商品详情
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BD Pharmingen™
Mouse (QC Testing)
Armenian Hamster IgG3, λ1
Vβ 8.3 TCR-expressing mouse Th1 clone 3.L2
Flow cytometry (Routinely Tested)
0.2 mg/ml
21608
AB_394980
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO


准备和存储

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

推荐的实验流程

For flow cytometry of cell suspensions from peripheral lymphoid tissues, it is recommended that multicolor staining be performed to distinguish T lymphocytes from non-T cells.

商品通知

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  3. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
  4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  5. Although hamster immunoglobulin isotypes have not been well defined, BD Biosciences Pharmingen has grouped Armenian and Syrian hamster IgG monoclonal antibodies according to their reactivity with a panel of mouse anti-hamster IgG mAbs. A table of the hamster IgG groups, Reactivity of Mouse Anti-Hamster Ig mAbs, may be viewed at http://www.bdbiosciences.com/documents/hamster_chart_11x17.pdf.
553664 Rev. 11
抗体详情
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1B3.3

The 1B3.3 antibody reacts with the Vβ 8.3 T-cell Receptor (TCR), but not the Vβ 8.1 nor Vβ 8.2 TCR, of mice having the b haplotype (e.g., A, AKR, BALB/c, CBA/Ca, CBA/J, C3H/He, C57BL, C58, DBA/1, DBA/2) of the Tcrb gene complex. The Tcrb-V8 subfamily gene loci are deleted in mice having the a (e.g., C57BR, C57L, SJL, SWR) or c (e.g., RIII) haplotype. Vβ 8.3 is one of the restricted TCR β chains expressed by NK-T cells. Staphylococcal enterotoxin B, in association with antigen-presenting cells expressing I-A and/or I-E, stimulates lymphocytes bearing Vβ 8 TCR and selectively eliminates those T cells in vivo. Vβ 8.3 TCR-bearing T-cell clones can be activated by 1B3.3 mAb. This hamster mAb to a mouse leukocyte antigen does not cross-react with rat leukocytes.

553664 Rev. 11
格式详情
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PE
R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
altImg
PE
Yellow-Green 488 nm, 532 nm, 561 nm
496 nm, 566 nm
576 nm
553664 Rev.11
报价单和参考
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研发参考 (6)

  1. Behlke MA, Chou HS, Huppi K, Loh DY. Murine T-cell receptor mutants with deletions of beta-chain variable region genes. Proc Natl Acad Sci U S A. 1986; 83(3):767-771. (Biology). 查看参考
  2. Haqqi TM, Banerjee S, Anderson GD, David CS. RIII S/J (H-2r). An inbred mouse strain with a massive deletion of T cell receptor V beta genes. J Exp Med. 1989; 169(6):1903-1909. (Biology). 查看参考
  3. Hugo P, Kappler JW, Godfrey DI, Marrack PC. Thymic epithelial cell lines that mediate positive selection can also induce thymocyte clonal deletion. J Immunol. 1994; 52(3):1022-1031. (Biology). 查看参考
  4. Kersh GJ, Donermeyer DL, Frederick KE, White JM, Hsu BL, Allen PM. TCR transgenic mice in which usage of transgenic alpha- and beta-chains is highly dependent on the level of selecting ligand. J Immunol. 1998; 161(2):585-593. (Immunogen). 查看参考
  5. Shinohara K, Ikarashi Y, Maruoka H, et al. Functional and phenotypical characteristics of hepatic NK-like T cells in NK1.1-positive and -negative mouse strains. Eur J Immunol. 1999; 29(6):1871-1878. (Biology). 查看参考
  6. White J, Herman A, Pullen AM, Kubo R, Kappler JW, Marrack P. The V beta-specific superantigen staphylococcal enterotoxin B: stimulation of mature T cells and clonal deletion in neonatal mice. Cell. 1989; 56(1):27-35. (Biology). 查看参考
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553664 Rev. 11

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