BD Pharmingen™ PE Goat Anti-Mouse Ig (Multiple Adsorption)

As a second-step reagent for staining rat spleen and bone marrow leukocytes, Cat. No. 550589 is reactive with mouse primary antibodies having the IgG1, IgG2a, IgG2b, IgG3, IgM, and IgA isotypes. Minimal background staining of rat leukocytes occurs in the absence of primary antibody. In addition, it stains mouse peripheral B lymphocytes with little non-specific staining of other splenic leukocytes. Therefore, it is useful as a primary reagent in immunofluorescent staining of mouse B cells and antibody-producing cells and as a secondary reagent for staining rat leukocytes with mouse Ig primary antibodies. However, we have observed that the reactivity of polyclonal second-step antibodies to mouse or rat IgM may be reduced after adsorption against Ig of rat or mouse, respectively. Because this anti-mouse Ig antibody was adsorbed with rat Ig, it may be weakly with some mouse IgM primary antibodies. In those cases, we recommend anti-mouse IgM mAb R6-60.2 (Cat. No. 553409) or anti-mouse Ig κ light chain mAb 187.1 (Cat. No. 559940). For optimal staining of human leukocytes, with mouse IgG and IgM primary antibodies, we recommend FITC-conjugated goat anti-mouse IgG + IgM (human-, bovine-, and horse-adsorbed, Cat. No. 555988).

1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
3. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.