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      PE-CF594 Mouse Anti-Islet-1
      PE-CF594 Mouse Anti-Islet-1
      Flow cytometric analysis of Islet-1 expression in Mouse pancreatic tumor (Insulinoma) cells.  Cells from the Mouse Beta-TC-6 (Insulinoma, ATCC© CRL-3605™) cell line were fixed with BD Cytofix™ Fixation Buffer (Cat. No. 554655) and permeabilized with BD Phosflow™ Perm Buffer III (Cat. No. 558050). The cells were then stained with either BD Horizon™ PE-CF594 Mouse IgG1, κ isotype control (Cat. No. 562292; dashed line histogram) or BD Horizon™ PE-CF594 Mouse Anti-Islet-1 antibody (Cat. No. 570983/571054; solid line histogram) at 0.5 µg/test. The fluorescence histogram showing Islet-1 expression (or Ig Isotype control staining) was derived was from gated events with the forward and side light-scatter characteristics of intact cells. Flow cytometry and data analysis were performed using a BD LSRFortessa™ X-20 Cell Analyzer System and FlowJo™ Software. Data shown on this Technical Data Sheet are not lot specific.
      PE-CF594 Mouse Anti-Islet-1
      Flow cytometric analysis of Islet-1 expression in Mouse pancreatic tumor (Insulinoma) cells.  Cells from the Mouse Beta-TC-6 (Insulinoma, ATCC© CRL-3605™) cell line were fixed with BD Cytofix™ Fixation Buffer (Cat. No. 554655) and permeabilized with BD Phosflow™ Perm Buffer III (Cat. No. 558050). The cells were then stained with either BD Horizon™ PE-CF594 Mouse IgG1, κ isotype control (Cat. No. 562292; dashed line histogram) or BD Horizon™ PE-CF594 Mouse Anti-Islet-1 antibody (Cat. No. 570983/571054; solid line histogram) at 0.5 µg/test. The fluorescence histogram showing Islet-1 expression (or Ig Isotype control staining) was derived was from gated events with the forward and side light-scatter characteristics of intact cells. Flow cytometry and data analysis were performed using a BD LSRFortessa™ X-20 Cell Analyzer System and FlowJo™ Software. Data shown on this Technical Data Sheet are not lot specific.
      商品详情
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      BD Horizon™
      ISL-1, ISL1, Islet-2, ISL-2, ISL2
      Mouse (QC Testing), Human (Tested in Development)
      Mouse IgG1, κ
      Human Islet-1 Recombinant Protein
      Intracellular staining (flow cytometry) (Routinely Tested)
      0.2 mg/ml
      Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
      RUO


      准备和存储

      The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to the dye under optimum conditions and unconjugated antibody and free dye were removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
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      推荐的实验流程

      BD® CompBeads can be used as surrogates to assess fluorescence spillover (compensation).  When fluorochrome conjugated antibodies are bound to BD® CompBeads, they have spectral properties very similar to cells.  However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls.  It is strongly recommended that when using a reagent for the first time, users compare the spillover on cells and BD® CompBeads to ensure that BD® CompBeads are appropriate for your specific cellular application.

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      商品通知

      1. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
      2. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
      3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
      4. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
      5. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
      6. An isotype control should be used at the same concentration as the antibody of interest.
      7. Texas Red is a registered trademark of Molecular Probes, Inc., Eugene, OR.
      8. When excited by the yellow-green (561-nm) laser, the fluorescence may be brighter than when excited by the blue (488-nm) laser.
      9. Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
      10. Because of the broad absorption spectrum of the tandem fluorochrome, extra care must be taken when using multi-laser cytometers, which may directly excite both PE and CF™594.
      11. Please observe the following precautions: We recommend that special precautions be taken (such as wrapping vials, tubes, or racks in aluminum foil) to protect exposure of conjugated reagents, including cells stained with those reagents, to any room illumination. Absorption of visible light can significantly affect the emission spectra and quantum yield of tandem fluorochrome conjugates.
      12. Species cross-reactivity detected in product development may not have been confirmed on every format and/or application.
      13. This product is provided under an Agreement between BIOTIUM and BD Biosciences. The manufacture, use, sale, offer for sale, or import of this product is subject to one or more patents or pending applications owned or licensed by Biotium, Inc. This product, and only in the amount purchased by buyer, may be used solely for buyer’s own internal research, in a manner consistent with the accompanying product literature. No other right to use, sell or otherwise transfer (a) this product, or (b) its components is hereby granted expressly, by implication or by estoppel. This product is for research use only. Diagnostic uses require a separate license from Biotium, Inc. For information on purchasing a license to this product including for purposes other than research, contact Biotium, Inc., 3159 Corporate Place, Hayward, CA 94545, Tel: (510) 265-1027. Fax: (510) 265-1352. Email: btinfo@biotium.com.
      14. CF™ is a trademark of Biotium, Inc.
      15. For U.S. patents that may apply, see bd.com/patents.
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      570983 Rev. 1
      抗体详情
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      Q11-465

      Islet-1 is a LIM-homeodomain transcription factor important for motor neuron differentiation and the formation of islet cells in the pancreas. Various heart cell types, such as cardiac muscle, the conduction system and endothelial cells in multiple heart tissue compartments during cardiogenesis, have been found to originate from Islet-1-positive cardiac precursor cells. Moreover, Islet-1-positive cells from differentiated human embryonic stem cell lines were found to be capable of self-renewal and expansion and could differentiate into the three major cell types of the heart.

      Western blot analyses of lysates from transfected cells demonstrate that the Q11-465 monoclonal antibody reacts with human Islet-1 (ISL-1, ISL1) and Islet-2 (ISL-2, ISL2), similar to the 4D5 clone (Tsuchida et al, 1994). Cross-reactivity with mouse Islet is also observed.

      570983 Rev. 1
      格式详情
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      PE-CF594
      BD Horizon™ PE-CF594 dye is a part of the BD PE family of dyes. This tandem fluorochrome is comprised of a R-Phycoerythrin (PE) donor that has excitation maxima (Ex Max) of 496-nm and 566-nm and an acceptor dye with an emission maximum (Em Max) at 615-nm. PE-CF594, driven by BD innovation, is designed to be excited by the blue (488-nm), Green (532-nm) and yellow-green (561-nm) lasers and detected using an optical filter centered near 615 nm (e.g., a 610/20-nm bandpass filter). The donor dye can be excited by the Blue (488-nm), Green (532-nm) and yellow-green (561-nm) lasers and the acceptor dye can be excited by the green (532-nm) laser resulting in cross-laser excitation and fluorescence spillover. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
      altImg
      PE-CF594
      Yellow-Green 561 nm
      496 nm, 566 nm
      615 nm
      570983 Rev.1
      报价单和参考
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      View product citations for antibody "570983" on CiteAb

      研发参考 (7)

      1. Bu L, Jiang X, Martin-Puig S, et al. Human ISL1 heart progenitors generate diverse multipotent cardiovascular cell lineages. Nature. 2009; 460(7251):113-117. (Biology). 查看参考
      2. Ebert AD, Yu J, Rose FF Jr, et al. Induced pluripotent stem cells from a spinal muscular atrophy patient. Nature. 2009; 457(7227):277-280. (Biology). 查看参考
      3. Emre N, Vidal JG, Elia J, et al. The ROCK inhibitor Y-27632 improves recovery of human embryonic stem cells after fluorescence-activated cell sorting with multiple cell surface markers. PLoS ONE. 2010; 5(8):e12148. (Methodology: Cell differentiation). 查看参考
      4. Laugwitz KL, Moretti A, Caron L, Nakano A, Chien KR. Islet1 cardiovascular progenitors: a single source for heart lineages. Development. 2008; 135(2):193-205. (Biology). 查看参考
      5. Osumi N, Hirota A, Ohuchi H, et al. Pax-6 is involved in the specification of hindbrain motor neuron subtype. Development. 1997; 124(15):2961-2972. (Biology). 查看参考
      6. Tsuchida T, Ensini M, Morton SB, et al. Topographic organization of embryonic motor neurons defined by expression of LIM homeobox genes. Cell. 1994; 79(6):957-970. (Biology). 查看参考
      7. Xu C, Police S, Hassanipour M, et al. Efficient generation and cryopreservation of cardiomyocytes derived from human embryonic stem cells. 2011; 6(1):53-66. (Biology). 查看参考
      查看所有文件 (7) 查看更少内容
      570983 Rev. 1

      Please refer to Support Documents for Quality Certificates

       

      Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

       

      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

      For Research Use Only. Not for use in diagnostic or therapeutic procedures.