FITC Mouse Anti-Human HLA-DR, DP, DQ

BD Pharmingen™ FITC Mouse Anti-Human HLA-DR, DP, DQ

Name: FITC Mouse Anti-Human HLA-DR, DP, DQ
Brand: BD Pharmingen™
SKU: 562008

克隆 Tu39 (also known as TÜ39)

(RUO)

Flow cytometric analysis of HLA-DR, DP, DQ expression on Rhesus macaque (Macaca mulatta) peripheral blood granulocytes. Rhesus whole blood was stained with either FITC Mouse Anti-Human HLA-DR, DP, DQ (Cat. No. 550853/555558/562008; solid line histogram) or FITC Mouse IgG2a, κ Isotype Control (Cat. No. 556652; dashed line histogram). Erythrocytes were lysed with BD Pharm Lyse™ Lysing Buffer (Cat. No. 555899). Fluorescent histograms were derived from gated events with the side and forward light-scattering characteristics of viable granulocytes.

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科学资源

商品详情

Brand: BD Pharmingen™

Alternative Name: Tü39; MHC class II HLA-DR, DP, DQ

Reactivity: Rhesus,Cynomolgus,Baboon (QC Testing), Human (Tested in Development)

Isotype: Mouse IgG2a, κ

Application: Flow cytometry (Routinely Tested)

Vol. Per Test: 20 µl

RRID: AB_393926

Storage Buffer: Aqueous buffered solution containing BSA and ≤0.09% sodium azide.

Regulatory Status: RUO

准备和存储

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with FITC under optimum conditions, and unreacted FITC was removed.

商品通知

This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
Source of all serum proteins is from USDA inspected abattoirs located in the United States.
For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
Species cross-reactivity detected in product development may not have been confirmed on every format and/or application.
Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.

配套商品

Stain Buffer (FBS) RUO

尺寸 500 mL 品类编号 554656

Stain Buffer (BSA) RUO

尺寸 500 mL 品类编号 554657

Lysing Buffer RUO

尺寸 100 mL 品类编号 555899

FITC Mouse IgG2a, κ Isotype Control RUO

尺寸 50 Tests 品类编号 556652

FITC Mouse Anti-Human HLA-DR, DP, DQ RUO

尺寸 100 Tests 品类编号 555558

562008 Rev. 2

抗体详情

Tu39

The TU39 monoclonal antibody specifically recognizes human major histocompatibility (MHC) Class II HLA-DR, DP and most DQ antigens. These antigens are encoded by genes within the Human Leukocyte Antigen (HLA) Complex located on chromosome 6. MHC Class II antigens are transmembrane heterodimeric glycoproteins composed of α chain (36 kDa) and β chain (27 kDa) subunits. They are expressed primarily on antigen presenting cells which include dendritic cells, monocytes, macrophages, thymic epithelial cells, and B cells. They are also expressed on activated T cells. This molecule plays a major role in mediating cellular interactions during antigen presentation to CD4+ T lineage cells. The TU39 antibody is reportedly useful for immunophenotyping as well as functional studies including the inhibition of mixed lymphocyte reactions and antibody-mediated complement fixation on target cells.

562008 Rev. 2

格式详情

FITC

Fluorescein (FITC) is part of the BD blue family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 494-nm and an emission maximum (Em Max) at 518-nm. FITC is designed to be excited by the Blue laser (488-nm) and detected using an optical filter centered near 520 nm (e.g., a 530/30-nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.

格式: FITC

激发源: Blue 488 nm

最多激发源: 494 nm

最多发射源: 518 nm

562008 Rev.2

报价单和参考

研发参考 (1)

Barclay NA, Brown MH, Birkeland ML, et al, ed. The Leukocyte Antigen FactsBook. San Diego, CA: Academic Press; 1997.

562008 Rev. 2

Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.