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      BV480 Rabbit Anti-Human LDH
      BV480 Rabbit Anti-Human LDH
      Flow cytometric analysis of LDH expression in HeLa cells. Cells from the Human HeLa (Cervical adenocarcinoma, ATCC® CCL-2™) cell line were fixed with BD Cytofix™ Fixation Buffer (Cat. No. 554655) and permeabilized with BD Phosflow™ Perm Buffer III (Cat. No. 558050). The cells were then stained with BD Horizon™ BV480 Rabbit IgG Isotype Control (Cat. No. 571424; dashed line histogram) or BD Horizon™ BV480 Rabbit Anti-Human LDH antibody (Cat. No. 570945; solid line histogram) at 0.25 µg/test. The fluorescence histogram showing the expression of LDH (or Ig Isotype control staining) was derived from gated events with the forward and side light-scatter characteristics of intact cells. Flow cytometry and data analysis were performed using a BD LSRFortessa™ X-20 Cell Analyzer System and FlowJo™ Software. Data shown on this Technical Data Sheet are not lot specific.
      BV480 Rabbit Anti-Human LDH
      Flow cytometric analysis of LDH expression in HeLa cells. Cells from the Human HeLa (Cervical adenocarcinoma, ATCC® CCL-2™) cell line were fixed with BD Cytofix™ Fixation Buffer (Cat. No. 554655) and permeabilized with BD Phosflow™ Perm Buffer III (Cat. No. 558050). The cells were then stained with BD Horizon™ BV480 Rabbit IgG Isotype Control (Cat. No. 571424; dashed line histogram) or BD Horizon™ BV480 Rabbit Anti-Human LDH antibody (Cat. No. 570945; solid line histogram) at 0.25 µg/test. The fluorescence histogram showing the expression of LDH (or Ig Isotype control staining) was derived from gated events with the forward and side light-scatter characteristics of intact cells. Flow cytometry and data analysis were performed using a BD LSRFortessa™ X-20 Cell Analyzer System and FlowJo™ Software. Data shown on this Technical Data Sheet are not lot specific.
      商品详情
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      BD Horizon™
      LDH heart subunit; LDH muscle subunit; LDH testis subunit; LDH-H; LDH-M; LDH-X; LDHA; LDHB; LDHC
      Human (QC Testing)
      Rabbit IgG
      Not Reported
      Intracellular staining (flow cytometry) (Routinely Tested)
      0.2 mg/ml
      3939, 3945, 3948
      Aqueous buffered solution containing ≤0.09% sodium azide.
      RUO


      准备和存储

      The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to the dye under optimum conditions and unconjugated antibody and free dye were removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
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      推荐的实验流程

      BD® CompBeads can be used as surrogates to assess fluorescence spillover (compensation).  When fluorochrome conjugated antibodies are bound to BD® CompBeads, they have spectral properties very similar to cells.   However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls.  It is strongly recommended that when using a reagent for the first time, users compare the spillover on cells and BD® CompBeads to ensure that BD® CompBeads are appropriate for your specific cellular application.

      For optimal and reproducible results, BD Horizon Brilliant™ Stain Buffer should be used anytime BD Horizon Brilliant dyes are used in a multicolor flow cytometry panel.  Fluorescent dye interactions may cause staining artifacts which may affect data interpretation.  The BD Horizon Brilliant Stain Buffer was designed to minimize these interactions. When BD Horizon Brilliant Stain Buffer is used in in the multicolor panel, it should also be used in the corresponding compensation controls for all dyes to achieve the most accurate compensation. For the most accurate compensation, compensation controls created with either cells or beads should be exposed to BD Horizon Brilliant Stain Buffer for the same length of time as the corresponding multicolor panel. More information can be found in the Technical Data Sheet of the BD Horizon Brilliant Stain Buffer (Cat. No. 563794/566349) or the BD Horizon Brilliant Stain Buffer Plus (Cat. No. 566385).

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      商品通知

      1. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
      2. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
      3. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
      4. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
      5. An isotype control should be used at the same concentration as the antibody of interest.
      6. Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
      7. Human donor specific background has been observed in relation to the presence of anti-polyethylene glycol (PEG) antibodies, developed as a result of certain vaccines containing PEG, including some COVID-19 vaccines. We recommend use of BD Horizon Brilliant™ Stain Buffer in your experiments to help mitigate potential background. For more information visit https://www.bdbiosciences.com/en-us/support/product-notices.
      8. This rabbit monoclonal (RabMabTM) product is, in part, provided under an intellectual property license from Abcam. The purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product for buyer’s internal research use only (whether the buyer is an academic or for-profit entity) and buyer shall not be permitted to reverse engineer or otherwise perform any compositional, structural, functional or other analyses directed to learning the methodology, formulae, sequences, process, make-up or production of this product (or any portion thereof). The sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: (i) in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. If you, as the buyer, do not agree to these conditions, please leave the product unopened and return the contents of this package to BD Biosciences, 10975 Torreyana Road, San Diego, CA 92121. For information on purchasing a license to this product for purposes other than research, contact Abcam at partnerships@abcam.com.
      9. For U.S. patents that may apply, see bd.com/patents.
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      570945 Rev. 1
      抗体详情
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      EP1563Y

      The EP1563Y recombinant monoclonal antibody binds to Lactate dehydrogenase (LDH). This tetrameric cytoplasmatic enzyme belongs to the 2-hydroxy acid oxidoreductase family whose subunits are encoded by LDHA, LDHB, LDHC, and LDHD genes. EP1563Y specifically recognizes LDH-A, LDH-B and LDH-C. LDH-A and LDH-B can form 5 tetrameric isoenzymes: LDH-1 (highly expressed in heart and erythrocytes), LDH-2 (reticuloendothelial system, erythrocytes), LDH-3 (lungs), LDH-4 (kidneys) and LDH-5 (liver, skeletal muscle, brain). LDH-C is specifically expressed in the testis. LDH has a key role in the anaerobic metabolic pathway as it catalyzes the synthesis of lactate and pyruvate in a reversible reaction, serving as an important check point of gluconeogenesis and DNA metabolism. LDH is overexpressed by cells in certain diseases such as cancer and can be released into the bloodstream due to tissue damage caused by injury or disease.

      570945 Rev. 1
      格式详情
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      BV480
      The BD Horizon Brilliant Violet™ 480 (BV480) Dye is part of the BD Horizon Brilliant Violet™ family of dyes. This polymer-technology fluorochrome has an excitation maximum (Ex Max) of 440-nm and an emission maximum (Em Max) of 479-nm. Driven by BD innovation, BV480 is designed to be excited by the violet laser (405-nm) and detected using an optical filter centered near 480-nm (e.g., a 525/50 bandpass filter). The increased fluorescence intensity of BV480 and narrower emission spectra, make it a good alternative for BV510 or V500. Due to its excitation profile, BV480 will also has less cross-laser excitation with the UV laser, resulting in less spillover into UV channels compared to BV510. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
      altImg
      BV480
      Violet 405 nm
      440 nm
      479 nm
      570945 Rev.1
      报价单和参考
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      View product citations for antibody "570945" on CiteAb

      研发参考 (4)

      1. Adeva M, González-Lucán M, Seco M, Donapetry C. Enzymes involved in l-lactate metabolism in humans.. Mitochondrion. 2013; 13(6):615-29. (Biology). 查看参考
      2. Khan AA, Allemailem KS, Alhumaydhi FA, Gowder SJT, Rahmani AH. The Biochemical and Clinical Perspectives of Lactate Dehydrogenase: An Enzyme of Active Metabolism.. Endocr Metab Immune Disord Drug Targets. 2020; 20(6):855-868. (Biology). 查看参考
      3. Mishra D, Banerjee D. Lactate Dehydrogenases as Metabolic Links between Tumor and Stroma in the Tumor Microenvironment.. Cancers (Basel). 2019; 11(6):750. (Biology). 查看参考
      4. Shi Y, Pinto BM.. Human lactate dehydrogenase a inhibitors: a molecular dynamics investigation.. PLoS One. 2014; 9(1):e86365. (Biology). 查看参考
      查看所有文件 (4) 查看更少内容
      570945 Rev. 1

      Please refer to Support Documents for Quality Certificates

       

      Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

       

      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

      For Research Use Only. Not for use in diagnostic or therapeutic procedures.