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      BV480 Mouse Anti-Mouse TCR Vγ6
      BV480 Mouse Anti-Mouse TCR Vγ6
      Multicolor flow cytometric analysis of TCR Vγ6 expression on newborn Mouse thymocytes. Two-day old BALB/c Mouse thymocytes were isolated and then stained with APC Hamster Anti-Mouse CD3e antibody (Cat. No. 553066), PE Hamster Anti-Mouse γδ T-Cell Receptor (TCR γδ; Cat. No. 553178), and with either BD Horizon™ BV480 Mouse IgG1, κ Isotype Control (Cat. No. 565652; Left Plot) or BD Horizon™ BV480 Mouse Anti-Mouse TCR Vγ6 antibody (Cat. No. 570929/571001; Right Plot) at 0.5 µg/test. DAPI Solution (Cat. No. 564907) was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of TCR Vγ6 (or Ig Isotype control staining) versus TCR γδ was derived from gated events with the forward and side light-scatter characteristics of viable (DAPI-negative) CD3e-positive lymphocytes. Flow cytometry and data analysis were performed using a BD FACSymphony™ A5 SE Cell Analyzer System and FlowJo™ Software. Data shown on this Technical Data Sheet are not lot specific.
      BV480 Mouse Anti-Mouse TCR Vγ6
      Multicolor flow cytometric analysis of TCR Vγ6 expression on newborn Mouse thymocytes. Two-day old BALB/c Mouse thymocytes were isolated and then stained with APC Hamster Anti-Mouse CD3e antibody (Cat. No. 553066), PE Hamster Anti-Mouse γδ T-Cell Receptor (TCR γδ; Cat. No. 553178), and with either BD Horizon™ BV480 Mouse IgG1, κ Isotype Control (Cat. No. 565652; Left Plot) or BD Horizon™ BV480 Mouse Anti-Mouse TCR Vγ6 antibody (Cat. No. 570929/571001; Right Plot) at 0.5 µg/test. DAPI Solution (Cat. No. 564907) was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of TCR Vγ6 (or Ig Isotype control staining) versus TCR γδ was derived from gated events with the forward and side light-scatter characteristics of viable (DAPI-negative) CD3e-positive lymphocytes. Flow cytometry and data analysis were performed using a BD FACSymphony™ A5 SE Cell Analyzer System and FlowJo™ Software. Data shown on this Technical Data Sheet are not lot specific.
      商品详情
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      BD Horizon™
      TCR Vg6; TCR Vgamma6; TCR Vγ6; TRGV6; TcR Vγ4; Tcrg-V6; Trgv6
      Mouse (QC Testing)
      Mouse IgG1, κ
      Mouse TCR Vγ6 Peptide
      Flow cytometry (Routinely Tested)
      0.2 mg/ml
      21640
      Aqueous buffered solution containing ≤0.09% sodium azide.
      RUO


      准备和存储

      The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to the dye under optimum conditions and unconjugated antibody and free dye were removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
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      推荐的实验流程

         BD® CompBeads can be used as surrogates to assess fluorescence spillover (compensation).  When fluorochrome conjugated antibodies are bound to BD® CompBeads, they have spectral properties very similar to cells.   However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls.  It is strongly recommended that when using a reagent for the first time, users compare the spillover on cells and BD® CompBeads to ensure that BD® CompBeads are appropriate for your specific cellular application.

         For optimal and reproducible results, BD Horizon Brilliant™ Stain Buffer should be used anytime BD Horizon Brilliant dyes are used in a multicolor flow cytometry panel.  Fluorescent dye interactions may cause staining artifacts which may affect data interpretation.  The BD Horizon Brilliant Stain Buffer was designed to minimize these interactions. When BD Horizon Brilliant Stain Buffer is used in in the multicolor panel, it should also be used in the corresponding compensation controls for all dyes to achieve the most accurate compensation. For the most accurate compensation, compensation controls created with either cells or beads should be exposed to BD Horizon Brilliant Stain Buffer for the same length of time as the corresponding multicolor panel. More information can be found in the Technical Data Sheet of the BD Horizon Brilliant Stain Buffer (Cat. No. 563794/566349) or the BD Horizon Brilliant Stain Buffer Plus (Cat. No. 566385)

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      商品通知

      1. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
      2. Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
      3. For U.S. patents that may apply, see bd.com/patents.
      4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
      5. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
      6. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
      7. An isotype control should be used at the same concentration as the antibody of interest.
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      570929 Rev. 1
      抗体详情
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      1C10-1F7

      The 1C10-1F7 monoclonal antibody specifically recognizes the variable gamma 6 region of the γ subunit of the mouse γδ T cell receptor for antigen, TCR Vγ6 (using the Heilig and Tonegawa nomenclature for mouse TCR γ and δ chains). TCR Vγ6 is encoded by the Trgv6 (T cell receptor gamma, variable 6) gene element. TCR Vγ6 is expressed by a subset of TCR γδ+ thymocytes that appear from approximately embryonic day 15 to birth and express Vγ6 paired with Vδ1 γδ TCR. These invariant TCR Vγ6Vδ1+ T cells home to various tissues including the epithelia of the lungs, peritoneal cavity, uterus, testis, tongue as well as the dermis, lamina propria of the colon, and adipose tissue. They are are programmed to secrete IL-17 upon activation.  The 1C10-1F7 antibody has been found useful for immunofluorescent staining and flow cytometric analysis of TCR Vγ6+ T cells from C57BL/6 and BALB/c mice. It was also found useful for immunohistochemical analysis and in vivo blocking studies of TCR Vγ6+ γδ T cell function as well. This antibody is suitable for TCR Vγ6+ thymocyte and γδ T cell separations and analyzing TCR Vγ repertoires expressed by thymocytes, peripheral T cells, and T cell hybridomas in developmental and other experimental model systems. TCR Vγ6 is referred to as TCR Vγ4 using the Garman, Doherty, and Raulet nomenclature for mouse TCR γ and δ chains.

      570929 Rev. 1
      格式详情
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      BV480
      The BD Horizon Brilliant Violet™ 480 (BV480) Dye is part of the BD Horizon Brilliant Violet™ family of dyes. This polymer-technology fluorochrome has an excitation maximum (Ex Max) of 440-nm and an emission maximum (Em Max) of 479-nm. Driven by BD innovation, BV480 is designed to be excited by the violet laser (405-nm) and detected using an optical filter centered near 480-nm (e.g., a 525/50 bandpass filter). The increased fluorescence intensity of BV480 and narrower emission spectra, make it a good alternative for BV510 or V500. Due to its excitation profile, BV480 will also has less cross-laser excitation with the UV laser, resulting in less spillover into UV channels compared to BV510. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
      altImg
      BV480
      Violet 405 nm
      440 nm
      479 nm
      570929 Rev.1
      报价单和参考
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      View product citations for antibody "570929" on CiteAb

      研发参考 (4)

      1. Hatano S, Tun X, Noguchi N, et al. Development of a new monoclonal antibody specific to mouse Vγ6 chain.. Life Sci Alliance. 2019; 2(3):e201900363. (Immunogen: Flow cytometry, Functional assay, Immunofluorescence, Immunohistochemistry, In vivo exacerbation). 查看参考
      2. Monin L, Ushakov DS, Arnesen H, et al. γδ T cells compose a developmentally regulated intrauterine population and protect against vaginal candidiasis.. Mucosal Immunol. 2020; 13(6):969-981. (Biology). 查看参考
      3. Roark CL, Aydintug MK, Lewis J, et al. Subset-specific, uniform activation among V gamma 6/V delta 1+ gamma delta T cells elicited by inflammation.. J Leukoc Biol. 2004; 75(1):68-75. (Biology). 查看参考
      4. Tun X, Hatano S, Mine K, et al. Vγ6+ γδ T cells are critical for protection against infection by Escherichia coli in mice.. Eur J Immunol. 2021; 51(8):2093-2096. (Clone-specific: Flow cytometry). 查看参考
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      570929 Rev. 1

       

      Please refer to Support Documents for Quality Certificates


      Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

      For Research Use Only. Not for use in diagnostic or therapeutic procedures.