BV480 Mouse Anti-Human CD3
Multiparameter flow cytometric analysis of CD3 expression on human peripheral blood leucocyte populations. Human whole blood was stained with either BD Horizon™ BV480 Mouse IgG2a, κ Isotype Control (Cat. No. 566089; Left Plot) or BD Horizon™ BV480 Mouse Anti-Human CD3 antibody (Cat. No. 568216; Right Plot) at 0.125 µg/test. Erythrocytes were lysed with BD FACS™ Lysing Solution (Cat. No. 349202). The bivariate pseudocolor density plot showing the correlated expression of CD3 (or Ig Isotype control staining) versus side light-scatter (SSC-A) signals was derived from gated events with the forward and side light-scatter characteristics of intact leucocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa™ X-20 Cell Analyzer System and FlowJo™ software. Data shown on this Technical Data Sheet are not lot specific.
BD Horizon™ BV480 Mouse Anti-Human CD3
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