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      BV421 Rat Anti-Mouse Galectin-9
      BV421 Rat Anti-Mouse Galectin-9
      Flow cytometric analysis of Galectin-9 expression on mouse thymocytes. C57BL/6 mouse thymocytes were fixed and permeabilized with BD Cytofix/Cytoperm™ Fixation and Permeabilization Solution (Cat. No. 554722), and stained in BD Perm/Wash™ Buffer (Cat. No. 554723) with either BD Horizon™ BV421 Rat IgG2a, κ Isotype Control (Cat. No. 562602; dashed line histogram) or BD Horizon BV421 Rat Anti-Mouse Galectin-9 antibody (Cat. No. 566028; solid line histogram).  The fluorescence histogram showing Galectin-9 expression (or Ig Isotype control staining) was derived from gated events with the forward and side light-scatter characteristics of intact thymocytes. Flow cytometric analysis was performed using a BD FACSCanto™ II Flow Cytometer System.
      BV421 Rat Anti-Mouse Galectin-9
      Flow cytometric analysis of Galectin-9 expression on mouse thymocytes. C57BL/6 mouse thymocytes were fixed and permeabilized with BD Cytofix/Cytoperm™ Fixation and Permeabilization Solution (Cat. No. 554722), and stained in BD Perm/Wash™ Buffer (Cat. No. 554723) with either BD Horizon™ BV421 Rat IgG2a, κ Isotype Control (Cat. No. 562602; dashed line histogram) or BD Horizon BV421 Rat Anti-Mouse Galectin-9 antibody (Cat. No. 566028; solid line histogram).  The fluorescence histogram showing Galectin-9 expression (or Ig Isotype control staining) was derived from gated events with the forward and side light-scatter characteristics of intact thymocytes. Flow cytometric analysis was performed using a BD FACSCanto™ II Flow Cytometer System.
      商品详情
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      BD Horizon™
      Galectin9; Gal-9; Gal9; Lgals9; LEG9
      Mouse (QC Testing)
      Rat IgG2a, κ
      Mouse Galectin-9 Recombinant Protein
      Intracellular staining (flow cytometry) (Routinely Tested)
      0.2 mg/ml
      AB_2739472
      Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
      RUO


      准备和存储

      Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with BD Horizon BV421 under optimum conditions, and unconjugated antibody and free BD Horizon BV421 were removed.
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      推荐的实验流程

      For optimal and reproducible results, BD Horizon Brilliant Stain Buffer should be used anytime two or more BD Horizon Brilliant dyes are used in the same experiment. Fluorescent dye interactions may cause staining artifacts which may affect data interpretation. The BD Horizon Brilliant Stain Buffer was designed to minimize these interactions. More information can be found in the Technical Data Sheet of the BD Horizon Brilliant Stain Buffer (Cat. No. 563794).

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      商品通知

      1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
      2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
      3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
      4. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
      5. Pacific Blue™ is a trademark of Molecular Probes, Inc., Eugene, OR.
      6. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
      7. An isotype control should be used at the same concentration as the antibody of interest.
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      566028 Rev. 1
      抗体详情
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      RG9-35

      The RG9-35 monoclonal antibody specifically binds to Galectin 9 (Gal-9) which is encoded by Lgals9 (lectin, galactose binding, soluble 9). Galectin-9 is expressed within cells and can be conveyed to the cell surface and released in soluble form. It is expressed by various cell types including lymphocytes, thymocytes, macrophages, dendritic cells, astrocytes, mast cells, eosinophils, fibroblasts, epithelial cells, and endothelial cells. Its expression can be induced by cells in response to proinflammatory cytokines or infection by certain viruses. Galectin-9 is a ~ 40 kDa S-type lectin that has two carbohydrate recognition domains (CRDs) and forms homodimers. It binds to β-galactosides and can serve as a ligand for TIM-3 (CD366). Galectin-9 can play multiple roles in innate and adaptive immune responses including the activation of macrophage cytokine secretion and bactericidal functions, promotion of dendritic cell maturation, regulatory T cell expansion, and negative regulation of Th1, Th17, NK, and cytotoxic T cells. The RG9-35 antibody can reportedly block some biological activities of Galectin-9.

       

      The antibody was conjugated to BD Horizon BV421 which is part of the BD Horizon Brilliant™ Violet family of dyes. With an Ex Max of 407-nm and Em Max at 421-nm, BD Horizon BV421 can be excited by the violet laser and detected in the standard Pacific Blue™ filter set (eg, 450/50-nm filter). BD Horizon BV421 conjugates are very bright, often exhibiting a 10 fold improvement in brightness compared to Pacific Blue conjugates.

      566028 Rev. 1
      格式详情
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      BV421
      The BD Horizon Brilliant Violet™ 421 (BV421) Dye is part of the BD Horizon Brilliant Violet™ family of dyes. This polymer-technology based dye has an excitation maximum (Ex Max) of 407-nm and an emission maximum (Em Max) at 423-nm. Driven by BD innovation, BV421 is designed to be excited by the violet laser (405-nm) and detected using an optical filter centered near 420-nm (e.g., a 431/28-nm or 450/50-nm bandpass filter). BV421 is an ideal alternative for V450 as it is approximately ten times brighter with less spillover into the BV510/V500 detector. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
      altImg
      BV421
      Violet 405 nm
      407 nm
      423 nm
      566028 Rev.1
      报价单和参考
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      View product citations for antibody "566028" on CiteAb

      研发参考 (8)

      1. Chiba S, Baghdadi M, Akiba H, et al. Tumor-infiltrating DCs suppress nucleic acid-mediated innate immune responses through interactions between the receptor TIM-3 and the alarmin HMGB1.. Nat Immunol. 2012; 13(9):832-42. (Clone-specific: In vivo exacerbation). 查看参考
      2. Fukushima A1, Sumi T, Fukuda K, et al. Roles of galectin-9 in the development of experimental allergic conjunctivitis in mice.. Int Arch Allergy Immunol. 2008; 146(1):36-43. (Immunogen: Functional assay, In vivo exacerbation). 查看参考
      3. Jayaraman P, Sada-Ovalle I, Beladi S, et al. Tim3 binding to galectin-9 stimulates antimicrobial immunity.. J Exp Med. 2010; 207(11):2343-54. (Biology). 查看参考
      4. Seki M, Oomizu S, Sakata KM, et al. Galectin-9 suppresses the generation of Th17, promotes the induction of regulatory T cells, and regulates experimental autoimmune arthritis.. Clin Immunol. 2008; 127(1):78-88. (Biology). 查看参考
      5. Shimmura-Tomita M, Wang M, Taniguchi H, Akiba H, Yagita H, Hori J. Galectin-9-mediated protection from allo-specific T cells as a mechanism of immune privilege of corneal allografts.. PLoS ONE. 2013; 8(5):e63620. (Clone-specific: Blocking, Flow cytometry, Fluorescence microscopy, Immunofluorescence, Inhibition, In vivo exacerbation). 查看参考
      6. Thornley TB, Fang Z, Balasubramanian S, et al. Fragile TIM-4-expressing tissue resident macrophages are migratory and immunoregulatory.. J Clin Invest. 2014; 124(8):3443-54. (Clone-specific: Flow cytometry, Functional assay). 查看参考
      7. Wada J, Ota K, Kumar A, Wallner EI, Kanwar YS. Developmental regulation, expression, and apoptotic potential of galectin-9, a beta-galactoside binding lectin.. J Clin Invest. 1997; 99(10):2452-61. (Biology). 查看参考
      8. Zhu C, Anderson AC, Schubart A, et al. The Tim-3 ligand galectin-9 negatively regulates T helper type 1 immunity.. Nat Immunol. 2005; 6(12):1245-52. (Biology). 查看参考
      查看所有文件 (8) 查看更少内容
      566028 Rev. 1

      Please refer to Support Documents for Quality Certificates


      Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

      For Research Use Only. Not for use in diagnostic or therapeutic procedures.