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      BV421 Rat Anti-Mouse CD14
      BV421 Rat Anti-Mouse CD14
      Flow cytometric analysis of CD14 expression on J774A.1 cells.  Cells from the J774A (Mouse macrophage, ATCC® TIB-67™) cell line were stained with either BD Horizon™ BV421 Rat IgG2a, κ Isotype Control (Cat. No. 562602; dashed line histogram) or BD Horizon™ BV421 Rat Anti-Mouse CD14 antibody (Cat. No. 570369/570439; solid line histogram) at 0.25 µg/test. BD Via-Probe™ Cell Viability 7-AAD Solution (Cat. No. 555815/555816) was added to cells right before analysis. The fluorescence histogram showing CD14 expression (or Ig Isotype control staining) was derived from gated events with the forward and side light-scatter characteristics of viable (7-AAD-negative) cells. Flow cytometric analysis was performed using a BD LSRFortessa™ X-20 Cell Analyzer System and FlowJo™ Software. Data shown on this Technical Data Sheet are not lot specific.
      BV421 Rat Anti-Mouse CD14
      Multiparameter flow cytometric analysis of CD14 expression on Mouse thioglycolate-elicited peritoneal exudate cell populations.  C57BL/6 Mouse peritoneal exudate cells were isolated 3 days post-stimulation by intraperitoneal injection of thioglycolate solution (Thio-PEC) and were preincubated with BD Pharmingen™ Purified Rat Anti-Mouse CD16/CD32 antibody (Mouse BD Fc Block™) [Cat. No. 553141/553142].The cells were then stained with either BD Horizon™ BV421 Rat IgG2a, κ Isotype Control (Cat. No. 562602; Left Plot) or BD Horizon™ BV421 Rat Anti-Mouse CD14 antibody (Cat. No. 570369/570439; Right Plot) at 0.25 µg/test. BD Via-Probe™ Cell Viability 7-AAD Solution (Cat. No. 555815/555816) was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD14 (or Ig Isotype control staining) versus side-light scatter (SSC-A) signals was derived from gated events with the forward and side-light scatter characteristics of viable (7-AAD-negative) Thio-PECs. Flow cytometry and data analysis were performed using a BD LSRFortessa™ X-20 Cell Analyzer System and FlowJo™ Software. Data shown on this Technical Data Sheet are not lot specific.
      BV421 Rat Anti-Mouse CD14 BV421 Rat Anti-Mouse CD14
      Flow cytometric analysis of CD14 expression on J774A.1 cells.  Cells from the J774A (Mouse macrophage, ATCC® TIB-67™) cell line were stained with either BD Horizon™ BV421 Rat IgG2a, κ Isotype Control (Cat. No. 562602; dashed line histogram) or BD Horizon™ BV421 Rat Anti-Mouse CD14 antibody (Cat. No. 570369/570439; solid line histogram) at 0.25 µg/test. BD Via-Probe™ Cell Viability 7-AAD Solution (Cat. No. 555815/555816) was added to cells right before analysis. The fluorescence histogram showing CD14 expression (or Ig Isotype control staining) was derived from gated events with the forward and side light-scatter characteristics of viable (7-AAD-negative) cells. Flow cytometric analysis was performed using a BD LSRFortessa™ X-20 Cell Analyzer System and FlowJo™ Software. Data shown on this Technical Data Sheet are not lot specific.
      Multiparameter flow cytometric analysis of CD14 expression on Mouse thioglycolate-elicited peritoneal exudate cell populations.  C57BL/6 Mouse peritoneal exudate cells were isolated 3 days post-stimulation by intraperitoneal injection of thioglycolate solution (Thio-PEC) and were preincubated with BD Pharmingen™ Purified Rat Anti-Mouse CD16/CD32 antibody (Mouse BD Fc Block™) [Cat. No. 553141/553142].The cells were then stained with either BD Horizon™ BV421 Rat IgG2a, κ Isotype Control (Cat. No. 562602; Left Plot) or BD Horizon™ BV421 Rat Anti-Mouse CD14 antibody (Cat. No. 570369/570439; Right Plot) at 0.25 µg/test. BD Via-Probe™ Cell Viability 7-AAD Solution (Cat. No. 555815/555816) was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD14 (or Ig Isotype control staining) versus side-light scatter (SSC-A) signals was derived from gated events with the forward and side-light scatter characteristics of viable (7-AAD-negative) Thio-PECs. Flow cytometry and data analysis were performed using a BD LSRFortessa™ X-20 Cell Analyzer System and FlowJo™ Software. Data shown on this Technical Data Sheet are not lot specific.
      商品详情
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      BD Horizon™
      Cd14; LPS Receptor
      Mouse (QC Testing)
      Rat IgG2a, κ
      Mouse CD14-transfected Cells
      Flow cytometry (Routinely Tested)
      0.2 mg/ml
      12475
      Aqueous buffered solution containing ≤0.09% sodium azide.
      RUO


      准备和存储

      The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to the dye under optimum conditions and unconjugated antibody and free dye were removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
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      推荐的实验流程

      BD® CompBeads can be used as surrogates to assess fluorescence spillover (compensation).  When fluorochrome conjugated antibodies are bound to BD® CompBeads, they have spectral properties very similar to cells.   However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls.  It is strongly recommended that when using a reagent for the first time, users compare the spillover on cells and BD® CompBeads to ensure that BD® CompBeads are appropriate for your specific cellular application.

      For optimal and reproducible results, BD Horizon Brilliant Stain Buffer should be used anytime BD Horizon Brilliant dyes are used in a multicolor flow cytometry panel.  Fluorescent dye interactions may cause staining artifacts which may affect data interpretation.  The BD Horizon Brilliant Stain Buffer was designed to minimize these interactions. When BD Horizon Brilliant Stain Buffer is used in in the multicolor panel, it should also be used in the corresponding compensation controls for all dyes to achieve the most accurate compensation. For the most accurate compensation, compensation controls created with either cells or beads should be exposed to BD Horizon Brilliant Stain Buffer for the same length of time as the corresponding multicolor panel. More information can be found in the Technical Data Sheet of the BD Horizon Brilliant Stain Buffer (Cat. No. 563794/566349) or the BD Horizon Brilliant Stain Buffer Plus (Cat. No. 566385).

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      商品通知

      1. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
      2. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
      3. An isotype control should be used at the same concentration as the antibody of interest.
      4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
      5. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
      6. Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
      7. For U.S. patents that may apply, see bd.com/patents.
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      570369 Rev. 1
      抗体详情
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      Sa14-2

      The SA14-2 monoclonal antibody specifically recognizes mouse CD14. CD14 is an ~55-kDa glycosyl phosphatidyl inositol (GPI)-linked cell-surface glycoprotein that is encoded by Cd14. CD14 is expressed on macrophages, granulocytes, dendritic cells, hepatocytes, and Kupffer cells. CD14 is also expressed in a soluble form (sCD14). CD14 is a leucine-rich repeat-containing pattern recognition receptor which serves as coreceptor for bacterial lipopolysaccharide (LPS) from gram-negative bacteria. CD14 forms a complex with lipopolysaccharide (LPS), an endotoxin from gram-negative bacteria, with LPS-binding protein (LBP, a plasma protein). The LPS-LPB-CD14 complex can then associate with TLR4-MD-2 which transduces intracellular signals. In this way, CD14 can play roles in innate cellular anti-bacterial immune and inflammatory responses, such as through the stimulation of cellular IL-1 and TNF production, and in extreme cases, the development of endotoxic shock.

      570369 Rev. 1
      格式详情
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      BV421
      The BD Horizon Brilliant Violet™ 421 (BV421) Dye is part of the BD Horizon Brilliant Violet™ family of dyes. This polymer-technology based dye has an excitation maximum (Ex Max) of 407-nm and an emission maximum (Em Max) at 423-nm. Driven by BD innovation, BV421 is designed to be excited by the violet laser (405-nm) and detected using an optical filter centered near 420-nm (e.g., a 431/28-nm or 450/50-nm bandpass filter). BV421 is an ideal alternative for V450 as it is approximately ten times brighter with less spillover into the BV510/V500 detector. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
      altImg
      BV421
      Violet 405 nm
      407 nm
      423 nm
      570369 Rev.1
      报价单和参考
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      View product citations for antibody "570369" on CiteAb

      研发参考 (3)

      1. Akashi S, Saitoh S, Wakabayashi Y, et al. Lipopolysaccharide interaction with cell surface Toll-like receptor 4-MD-2: higher affinity than that with MD-2 or CD14. J Exp Med. 2003; 198(7):1035-1042. (Biology). 查看参考
      2. Ghosh M, Subramani J, Rahman MM, Shapiro LH. CD13 restricts TLR4 endocytic signal transduction in inflammation.. J Immunol. 2015; 194(9):4466-76. (Clone-specific: Flow cytometry). 查看参考
      3. Kiyokawa T, Akashi-Takamura S, Shibata T, et al. A single base mutation in the PRAT4A gene reveals differential interaction of PRAT4A with Toll-like receptors.. Int Immunol. 2008; 20(11):1407-15. (Immunogen: Flow cytometry). 查看参考
      570369 Rev. 1

      Please refer to Support Documents for Quality Certificates


      Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

      For Research Use Only. Not for use in diagnostic or therapeutic procedures.