BV421 Mouse Anti-Human IL-4

BD Horizon™ BV421 Mouse Anti-Human IL-4

Name: BV421 Mouse Anti-Human IL-4
Brand: BD Horizon™
SKU: 562986

克隆 8D4-8

(RUO)

查看所有格式

Protocol Library

Scientific Resources

Multicolor flow cytometric analysis of IL-4 expressed in stimulated human lymphocytes. HiCK-2 cells (Human Cytokine Positive Control Cells; Cat. No. 555062) were permeabilized with BD Perm/Wash™ Buffer (Cat. No. 554723). The cells were then stained with Alexa Fluor® 647 Mouse Anti-Human CD4 antibody (Cat No. 557707) and with either BD Horizon™ BV421 Mouse IgG1 κ Isotype Control (Cat No. 562438, Left Panel) or BD Horizon™ BV421 Mouse Anti-Human IL-4 antibody (Cat No. 562986, Right Panel) by using the BD Biosciences Intracellular Cytokine Staining protocol. Two-color flow cytometric dot plots showing the correlated expression patterns of IL-4 (or Ig Isotype control staining) versus CD4 were derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometry was performed using a BD™ LSR II Flow Cytometer System.

光谱浏览器

协议库

科学资源

商品详情

Brand: BD Horizon™

Alternative Name: Interleukin-4; IL4; BCGF-1; BSF-1; Lymphocyte stimulatory factor 1

Reactivity: Human (QC Testing)

Isotype: Mouse IgG1, κ

Immunogen: Recombinant Human IL-4

Application: Intracellular staining (flow cytometry) (Routinely Tested)

Vol. Per Test: 5 µl

RRID: AB_2737933

Storage Buffer: Aqueous buffered solution containing BSA and ≤0.09% sodium azide.

Regulatory Status: RUO

准备和存储

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with BD Horizon BV421 under optimum conditions, and unconjugated antibody and free BD Horizon BV421 were removed.

商品通知

This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
An isotype control should be used at the same concentration as the antibody of interest.
Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
Source of all serum proteins is from USDA inspected abattoirs located in the United States.
Pacific Blue™ is a trademark of Molecular Probes, Inc., Eugene, OR.
Brilliant Violet™ 421 is a trademark of Sirigen.
For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.

配套商品

Stain Buffer (FBS) RUO

尺寸 500 mL 品类编号 554656

Perm/Wash Buffer RUO

尺寸 100 mL 品类编号 554723

BV421 Mouse IgG1, k Isotype Control RUO

尺寸 50 µg 品类编号 562438

HiCK-2 Human Cytokine Positive Control Cells RUO

尺寸 1 mL 品类编号 555062

Alexa Fluor®647 Mouse Anti-Human CD4 RUO

尺寸 100 Tests 品类编号 557707

562986 Rev. 2

抗体详情

8D4-8

The 8D4-8 monoclonal antibody reacts with human interleukin-4 (IL-4). The immunogen used to raise the 8D4-8 hybridoma was recombinant human IL-4. The 8D4-8 antibody binds to an epitope that is different than the epitope recognized by the MP4-25D2 antibody (Cat. No. 554485).

Clone 8D4-8 displays an increased amount of non-specific binding to dead cells when compared to the clone MP4-25D2. It is recommended to use a fixable viability dye in conjunction with this clone.

The antibody was conjugated to BD Horizon™ BV421 which is part of the BD Horizon™ Brilliant Violet™ family of dyes. With an Ex Max of 407-nm and Em Max at 421-nm, BD Horizon™ BV421 can be excited by the violet laser and detected in the standard Pacific Blue™ filter set (eg, 450/50-nm filter). BD Horizon™ BV421 conjugates are very bright, often exhibiting a 10 fold improvement in brightness compared to Pacific Blue™ conjugates.

562986 Rev. 2

格式详情

BV421

The BD Horizon Brilliant Violet™ 421 (BV421) Dye is part of the BD Horizon Brilliant Violet™ family of dyes. This polymer-technology based dye has an excitation maximum (Ex Max) of 407-nm and an emission maximum (Em Max) at 423-nm. Driven by BD innovation, BV421 is designed to be excited by the violet laser (405-nm) and detected using an optical filter centered near 420-nm (e.g., a 431/28-nm or 450/50-nm bandpass filter). BV421 is an ideal alternative for V450 as it is approximately ten times brighter with less spillover into the BV510/V500 detector. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.

格式: BV421

激发源: Violet 405 nm

最多激发源: 407 nm

最多发射源: 423 nm

562986 Rev.2

报价单和参考

研发参考 (2)

Bird C, Wadhwa M, Thorpe R. Development of immunoassays for human interleukin 3 and interleukin 4, some of which discriminate between different recombinant DNA-derived molecules. Cytokine. 1991; 3(6):562-567. (Immunogen: Neutralization, Radioimmunoassay). 查看参考
Prussin C, Metcalfe DD. Detection of intracytoplasmic cytokine using flow cytometry and directly conjugated anti-cytokine antibodies. J Immunol Methods. 1995; 188(1):117-128. (Clone-specific: Flow cytometry, IC/FCM Block). 查看参考

562986 Rev. 2

Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.