Flow cytometric analysis of Ly-6G expression on mouse bone-marrow leucocytes. BALB/c mouse bone-marrow cells were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody (Mouse BD Fc Block™) (Cat. No. 553141/553142). The cells were then stained with either Biotin Rat IgG2a, κ Isotype Control (Cat. No. 553928; dashed line histograms) or Biotin Rat Anti-Mouse Ly-6G antibody (Cat. No. 567599; solid line histograms) at 1 µg/test. The cells were then counterstained with PE Streptavidin (Cat. No. 554061). DAPI Solution (Cat. No. 564907) was added to cells right before analysis. The fluorescence histograms showing Ly-6G expression (or Ig Isotype control staining) were derived from gated events with the light scattering characteristics of viable (DAPI-negative) lymphoid (Left Plot) or myeloid (Right Plot) cells. Flow cytometry and data analysis were performed using a BD LSRFortessa™ Cell Analyzer System and FlowJo™ software. Data shown on this Technical Data Sheet are not lot specific.
Flow cytometric analysis of Ly-6G expression on mouse bone-marrow leucocytes. BALB/c mouse bone-marrow cells were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody (Mouse BD Fc Block™) (Cat. No. 553141/553142). The cells were then stained with either Biotin Rat IgG2a, κ Isotype Control (Cat. No. 553928; dashed line histograms) or Biotin Rat Anti-Mouse Ly-6G antibody (Cat. No. 567599; solid line histograms) at 1 µg/test. The cells were then counterstained with PE Streptavidin (Cat. No. 554061). DAPI Solution (Cat. No. 564907) was added to cells right before analysis. The fluorescence histograms showing Ly-6G expression (or Ig Isotype control staining) were derived from gated events with the light scattering characteristics of viable (DAPI-negative) lymphoid (Left Plot) or myeloid (Right Plot) cells. Flow cytometry and data analysis were performed using a BD LSRFortessa™ Cell Analyzer System and FlowJo™ software. Data shown on this Technical Data Sheet are not lot specific.
商品详情
BD Pharmingen™
Ly6g; Lymphocyte antigen 6G; Lymphocyte antigen 6 complex, locus G; Gr1
Mouse (QC Testing)
Rat LEW, also known as Lewis IgG2a, κ
Ly-6G-transfected EL4J Cell Line
Flow cytometry (Tested During Development)
0.5 mg/ml
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO
准备和存储
Store undiluted at 4°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed.
推荐的实验流程
商品通知
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- An isotype control should be used at the same concentration as the antibody of interest.
- Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
配套商品
Stain Buffer (BSA) RUO
尺寸
500 mL
品类编号
554657
Purified Rat Anti-Mouse CD16/CD32 (Mouse BD Fc Block™) RUO
尺寸
0.5 mg
品类编号
553142
Biotin Rat IgG2a κ Isotype Control RUO
尺寸
0.25 mg
品类编号
553928
PE Streptavidin RUO
尺寸
0.5 mg
品类编号
554061
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DAPI Solution RUO
尺寸
1 mg
品类编号
564907
567599 Rev. 1
抗体详情
1A8
The 1A8 monoclonal antibody specifically binds to Ly-6G, a 21-25-kDa GPI-anchored protein. In the bone marrow, Ly6G is expressed on the majority of the largest cells, predominantly granulocytes, but not on lymphoid or erythroid cells. In the periphery, it is expressed on granulocytes. The mAb RB6-8C5 recognizes both Ly-6G and Ly-6C and blocks the binding of mAb 1A8 to Ly-6G.
567599 Rev. 1
格式详情
Biotin
Biotin is a ubiquitous co-factor (also known as Vitamin B7) that has many properties that make it extremely useful for molecular biology. Biotin has an extremely high affinity for the Avidin family of proteins (Kd = 10-15 M), making it the perfect tool to link two molecules. Biotin labeled antibodies can be combined with any number of Avidin-conjugated probes in order to customize an assay to a particular need. This is especially useful in the case of magnetic cell separation using streptavidin/magnetic bead conjugates, or in the case of flow cytometry using streptavidin/fluorophore conjugates.
Biotin
567599 Rev.1
报价单和参考
研发参考 (3)
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Fleming TJ, Fleming ML, Malek TR. Selective expression of Ly-6G on myeloid lineage cells in mouse bone marrow. RB6-8C5 mAb to granulocyte-differentiation antigen (Gr-1) detects members of the Ly-6 family. J Immunol. 1993; 151(5):2399-2408. (Immunogen: Flow cytometry, Immunoprecipitation). 查看参考
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Fleming TJ, Malek TR. Multiple glycosylphosphatidylinositol-anchored Ly-6 molecules and transmembrane Ly-6E mediate inhibition of IL-2 production. J Immunol. 1994; 153(5):1955-1962. (Clone-specific: Flow cytometry, Functional assay, Inhibition). 查看参考
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Fleming TJ, O'HUigin C, Malek TR. Characterization of two novel Ly-6 genes. Protein sequence and potential structural similarity to alpha-bungarotoxin and other neurotoxins. J Immunol. 1993; 150(12):5379-5390. (Biology). 查看参考
567599 Rev. 1
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.
