Biotin Rat Anti-Mouse CD115 (CSF-1R)
Multicolor flow cytometric analysis of CD115 (CSF-1R) expression on mouse bone marrow cells. C57BL/6 mouse bone marrow cells were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody (Mouse BD Fc Block™) (Cat. No. 553141/553142). The cells were stained with BD Horizon™ BV421 Rat Anti-Mouse CD11b antibody (Cat. No. 562605) and either Biotin Rat IgG1, κ Isotype Control (Cat. No. 553923; Left Plot) or Biotin Rat Anti-Mouse CD115 (CSF-1R) antibody (Cat. No. 567598; Right Plot) at 0.25 µg/test, followed by PE Streptavidin (Cat. No. 554061). A bivariate pseudocolor density plot showing the correlated expression of CD115 (CSF-1R) [or Ig Isotype control staining] versus CD11b was derived from gated events with the forward and side-light scattering characteristics of intact bone marrow cells. Flow cytometry and data analysis were performed using a BD LSRFortessa™ Cell Analyzer System and FlowJo™ software. Data shown on this Technical Data Sheet are not lot specific.
BD Pharmingen™ Biotin Rat Anti-Mouse CD115 (CSF-1R)
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