Biotin Mouse Anti-Mouse Fcµ Receptor

BD Pharmingen™ Biotin Mouse Anti-Mouse Fcµ Receptor

Name: Biotin Mouse Anti-Mouse Fcµ Receptor
Brand: Biotin Mouse Anti-Mouse Fcµ Receptor
SKU: 566837

克隆 MM3 (RUO)

Two-color flow cytometric analysis of Fcμ Receptor expression on mouse splenic B cells. C57BL/6 (Upper Plots) or BALB/c (Lower Plots) mouse splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody (Mouse BD Fc Block™) (Cat. No. 553141/553142). The cells were then stained with APC Rat Anti-Mouse CD19 antibody (Cat. No. 550992/561738) and either Biotin Mouse IgG1, κ Isotype Control (Cat. No. 554680; Left Plots) or Biotin Mouse Anti-Mouse Fcµ Receptor antibody (Cat. No. 566837; Right Plots) at 2 μg/test. The cells were washed and then counterstained with PE Streptavidin (Cat. No. 554061). Two-color flow cytometric contour plots showing the correlated expression of Fcµ Receptor (or Ig Isotype control staining) versus CD19 were derived from gated events with the forward and side light-scatter characteristics of viable cells. Flow cytometry and data analysis were performed using a BD LSRFortessa™ Cell Analyzer System and FlowJo™ software. Data shown on this Technical Data Sheet are not lot specific.

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实验方案

科学资源

商品详情

Brand: BD Pharmingen™

Alternative Name: Fcmr; FcmuR; Fcμ Receptor; FcμR; Faim3; Toso

Reactivity: Mouse (QC Testing)

Isotype: Mouse IgG1, κ

Immunogen: Mouse FcµR Transfected Cell Line

Application: Flow cytometry (Routinely Tested)

Concentration: 0.5 mg/ml

RRID: AB_2869895

Storage Buffer: Aqueous buffered solution containing ≤0.09% sodium azide.

Regulatory Status: RUO

准备和存储

Store undiluted at 4°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed.

商品通知

Since applications vary, each investigator should titrate the reagent to obtain optimal results.
An isotype control should be used at the same concentration as the antibody of interest.
Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.

配套商品

Purified Rat Anti-Mouse CD16/CD32 (Mouse BD Fc Block™) RUO

尺寸 0.1 mg 品类编号 553141

Purified Rat Anti-Mouse CD16/CD32 (Mouse BD Fc Block™) RUO

尺寸 0.5 mg 品类编号 553142

Stain Buffer (FBS) RUO

尺寸 500 ML 品类编号 554656

Stain Buffer (BSA) RUO

尺寸 500 ML 品类编号 554657

Lysing Buffer RUO

尺寸 100 ML 品类编号 555899

PE Streptavidin RUO

尺寸 0.5 mg 品类编号 554061

查看详情

566837 Rev. 1

抗体详情

MM3

The MM3 monoclonal antibody specifically binds to the Fc receptor for IgM (Fcµ Receptor, FcµR), which is also known as Fas apoptosis inhibitory molecule 3 (Faim3) or Toso. The Fcµ Receptor is a transmembrane sialoglycoprotein that is encoded by Fcmr (Fc fragment of IgM receptor). This receptor contains an extracellular Ig-like domain that is homologous to two other IgM-binding receptors, the Fc receptor for IgM and polymeric IgA (Fcα/µR) and the polymeric Ig receptor (pIgR). Unlike these receptors, the Fcµ Receptor exhibits exclusive binding specificity for the Fc region of IgM. Fcµ Receptor expression is restricted to B lymphocytes. Although expressed by all mature B cell subsets, Fcµ Receptor levels are transiently downregulated by germinal center B cells. This receptor is not expressed by T cells, NK cells, dendritic cells, or myeloid cells. Fcmr gene ablation resulted in an increase of splenic B1 B cells but a decrease in marginal zone B cells in mutant versus wild type mice. The Fcµ Receptor is involved in the regulation of humoral immunity including IgM homeostasis and B cell survival.

566837 Rev. 1

格式详情

Biotin

Biotin is a ubiquitous co-factor (also known as Vitamin B7) that has many properties that make it extremely useful for molecular biology. Biotin has an extremely high affinity for the Avidin family of proteins (Kd = 10-15 M), making it the perfect tool to link two molecules. Biotin labeled antibodies can be combined with any number of Avidin-conjugated probes in order to customize an assay to a particular need. This is especially useful in the case of magnetic cell separation using streptavidin/magnetic bead conjugates, or in the case of flow cytometry using streptavidin/fluorophore conjugates.

格式: Biotin

566837 Rev.1

报价单和参考

View product citations for antibody "566837" on CiteAb

研发参考 (4)

Honjo K, Kubagawa Y, Jones DM, et al. Altered Ig levels and antibody responses in mice deficient for the Fc receptor for IgM (FcμR).. Proc Natl Acad Sci USA. 2012; 109(39):15882-7. (Immunogen: Flow cytometry, Immunoprecipitation). 查看参考
Honjo K, Kubagawa Y, Kubagawa H. Is Toso/IgM Fc receptor (FcμR) expressed by innate immune cells?. Proc Natl Acad Sci USA. 2013; 110(28):E2540-1. (Clone-specific: Flow cytometry). 查看参考
Kubagawa H, Oka S, Kubagawa Y, et al. The long elusive IgM Fc receptor, FcμR.. J Clin Immunol. 2014; 34 Suppl 1:S35-45. (Biology). 查看参考
Shima H, Takatsu H, Fukuda S, et al. Identification of TOSO/FAIM3 as an Fc receptor for IgM.. Int Immunol. 2010; 22(3):149-156. (Biology). 查看参考

查看所有文件 (4)

566837 Rev. 1

Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.