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Two-color flow cytometric analysis of Fcμ Receptor expression on mouse splenic B cells. C57BL/6 (Upper Plots) or BALB/c (Lower Plots) mouse splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody (Mouse BD Fc Block™) (Cat. No. 553141/553142). The cells were then stained with APC Rat Anti-Mouse CD19 antibody (Cat. No. 550992/561738) and either Biotin Mouse IgG1, κ Isotype Control (Cat. No. 554680; Left Plots) or Biotin Mouse Anti-Mouse Fcµ Receptor antibody (Cat. No. 566837; Right Plots) at 2 μg/test. The cells were washed and then counterstained with PE Streptavidin (Cat. No. 554061). Two-color flow cytometric contour plots showing the correlated expression of Fcµ Receptor (or Ig Isotype control staining) versus CD19 were derived from gated events with the forward and side light-scatter characteristics of viable cells. Flow cytometry and data analysis were performed using a BD LSRFortessa™ Cell Analyzer System and FlowJo™ software. Data shown on this Technical Data Sheet are not lot specific.
BD Pharmingen™ Biotin Mouse Anti-Mouse Fcµ Receptor
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- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- An isotype control should be used at the same concentration as the antibody of interest.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
配套商品
The MM3 monoclonal antibody specifically binds to the Fc receptor for IgM (Fcµ Receptor, FcµR), which is also known as Fas apoptosis inhibitory molecule 3 (Faim3) or Toso. The Fcµ Receptor is a transmembrane sialoglycoprotein that is encoded by Fcmr (Fc fragment of IgM receptor). This receptor contains an extracellular Ig-like domain that is homologous to two other IgM-binding receptors, the Fc receptor for IgM and polymeric IgA (Fcα/µR) and the polymeric Ig receptor (pIgR). Unlike these receptors, the Fcµ Receptor exhibits exclusive binding specificity for the Fc region of IgM. Fcµ Receptor expression is restricted to B lymphocytes. Although expressed by all mature B cell subsets, Fcµ Receptor levels are transiently downregulated by germinal center B cells. This receptor is not expressed by T cells, NK cells, dendritic cells, or myeloid cells. Fcmr gene ablation resulted in an increase of splenic B1 B cells but a decrease in marginal zone B cells in mutant versus wild type mice. The Fcµ Receptor is involved in the regulation of humoral immunity including IgM homeostasis and B cell survival.
研发参考 (4)
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Honjo K, Kubagawa Y, Jones DM, et al. Altered Ig levels and antibody responses in mice deficient for the Fc receptor for IgM (FcμR).. Proc Natl Acad Sci USA. 2012; 109(39):15882-7. (Immunogen: Flow cytometry, Immunoprecipitation). 查看参考
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Honjo K, Kubagawa Y, Kubagawa H. Is Toso/IgM Fc receptor (FcμR) expressed by innate immune cells?. Proc Natl Acad Sci USA. 2013; 110(28):E2540-1. (Clone-specific: Flow cytometry). 查看参考
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Kubagawa H, Oka S, Kubagawa Y, et al. The long elusive IgM Fc receptor, FcμR.. J Clin Immunol. 2014; 34 Suppl 1:S35-45. (Biology). 查看参考
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Shima H, Takatsu H, Fukuda S, et al. Identification of TOSO/FAIM3 as an Fc receptor for IgM.. Int Immunol. 2010; 22(3):149-156. (Biology). 查看参考
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