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Biotin Mouse Anti-Human CD271
Biotin Mouse Anti-Human CD271

Flow cytometric analysis of CD271 expression on REH Cells.  Cells from the human REH leukemia cell line (ATCC CRL-8286) were stained with either Biotin Mouse Anti-Human CD271 (Cat. No. 557195; solid line histogram) or Biotin Mouse IgG1 κ Isotype Control (Cat. No. 555747; dashed line histogram), followed by PE Streptavidin (Cat. No. 554061) second step.  Flow cytometric fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of viable cells.

Flow cytometric analysis of CD271 expression on REH Cells.  Cells from the human REH leukemia cell line (ATCC CRL-8286) were stained with either Biotin Mouse Anti-Human CD271 (Cat. No. 557195; solid line histogram) or Biotin Mouse IgG1 κ Isotype Control (Cat. No. 555747; dashed line histogram), followed by PE Streptavidin (Cat. No. 554061) second step.  Flow cytometric fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of viable cells.

商品详情
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BD Pharmingen™
NGFR; NGF Receptor; TNFRSF16
Human (QC Testing)
Mouse IgG1, κ
Human NGFR Recombinant Protein
Flow cytometry (Routinely Tested)
20 µl
VIII 80150
AB_396598
Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
RUO


准备和存储

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed.

商品通知

  1. This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
  2. An isotype control should be used at the same concentration as the antibody of interest.
  3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
  4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  5. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
  6. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
557195 Rev. 7
抗体详情
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C40-1457

The C40-1457 monoclonal antibody specifically recognizes CD271 that is also known as the nerve growth factor receptor (NGFR). CD271 is 75 kDa type I transmembrane glycoprotein likewise known as TNFRSF16 that belongs to the tumor necrosis factor receptor (TNFR) superfamily. CD271 has been found localized to neuronal axons, Schwann cells, and perineural cells of peripheral nerves. It is also expressed by some epithelial, mesenchymal and lymphoid tissues. NGFR is the receptor for nerve growth factor (NGF), a polypeptide that is essential for normal development of the nervous system. NGF promotes survival and differentiation of sympathetic and sensory neurons during embryological development of the peripheral nervous system. NGF binds to two distinctive surface receptors, the p/140[prototrk] and p75[NGFR]. High affinity binding of NGF requires that both receptor molecules be expressed. NGFR is expressed on human and rat lymphocytes. A subset of lymphoid cells in the spleen, lymph nodes, and follicular dendritic cells in germinal centers of reactive lymph nodes were found to express CD271. It has been reported that NGFR interaction with its ligand, NGF, may play a role in immunoregulation. NGF may also function as a B-cell growth factor.

557195 Rev. 7
格式详情
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Biotin
Biotin is a ubiquitous co-factor (also known as Vitamin B7) that has many properties that make it extremely useful for molecular biology. Biotin has an extremely high affinity for the Avidin family of proteins (Kd = 10-15 M), making it the perfect tool to link two molecules. Biotin labeled antibodies can be combined with any number of Avidin-conjugated probes in order to customize an assay to a particular need. This is especially useful in the case of magnetic cell separation using streptavidin/magnetic bead conjugates, or in the case of flow cytometry using streptavidin/fluorophore conjugates.
Biotin
557195 Rev.7
报价单和参考
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研发参考 (8)

  1. Brodie C, Gelfand EW. Functional nerve growth factor receptors on human B lymphocytes. Interaction with IL-2. J Immunol. 1992; 148(11):3492-3497. (Biology). 查看参考
  2. Chesa PG, Rettig WJ, Thomson TM, Old LJ, Melamed MR. Immunohistochemical analysis of nerve growth factor receptor expression in normal and malignant human tissues. J Histochem Cytochem. 1988; 36(4):383-389. (Biology). 查看参考
  3. Guesdon JL, Ternynck T, Avrameas S. The use of avidin-biotin interaction in immunoenzymatic techniques. J Histochem Cytochem. 1979; 27(8):1131-1139. (Biology). 查看参考
  4. Hempstead BL, Martin-Zanca D, Kaplan DR, Parada LF, Chao MV. High-affinity NGF binding requires coexpression of the trk proto-oncogene and the low-affinity NGF receptor. Nature. 1991; 350(6320):678-683. (Biology). 查看参考
  5. Holling TM, Bergevoet MW, Wilson L, et al. A Role for EZH2 in Silencing of IFN-γ Inducible MHC2TA Transcription in Uveal Melanoma. J Immunol. 2007; 179(8):5317-5325. (Biology). 查看参考
  6. Kanellopoulou C, Muljo SA, Dimitrov SD, et al. X chromosome inactivation in the absence of Dicer.. Proc Natl Acad Sci U S A. 2009; 106(4):1122-1127. (Biology). 查看参考
  7. Thompson SJ, Schatteman GC, Gown AM, Bothwell M. A monoclonal antibody against nerve growth factor receptor. Immunohistochemical analysis of normal and neoplastic human tissue. Am J Clin Pathol. 1989; 92(4):415-423. (Clone-specific). 查看参考
  8. Van Landuyt K, Lories R, Jones E, et al. Flow cytometric characterization of freshly isolated and culture expanded human synovial cell populations in patients with chronic arthritis. Arthritis Res Ther. 2001; 12(1):R15. (Clone-specific: Flow cytometry). 查看参考
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557195 Rev. 7

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For Research Use Only. Not for use in diagnostic or therapeutic procedures.