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BB515 Mouse Anti-Human CD11c
BB515 Mouse Anti-Human CD11c

Multiparameter flow cytometric analysis of CD11c expression on human peripheral blood leucocyte populations. Human peripheral blood (collected with heparin as the preferred anticoagulant rather than EDTA) was stained with either BD Horizon™ BB515 Mouse IgG1, κ Isotype Control (Cat. No. 564416; Left Plot) or BD Horizon™ BB515 Mouse Anti-Human CD11c antibody (Cat. No. 566835; Right Plot) at 0.5 μg/test. The erythrocytes were lysed with BD FACS™ Lysing Solution (Cat. No. 349202). The two-parameter pseudocolor density plot showing the correlated expression of CD11c (or Ig Isotype control staining) versus side light scatter signals (SSC-A) was derived from gated events with the forward and side light-scatter characteristics of intact leucocyte populations. Flow cytometry and data analysis were performed using a BD LSRFortessa™ X-20 Cell Analyzer System and FlowJo™ software. Data shown in this Technical Data Sheet are not lot specific.

Multiparameter flow cytometric analysis of CD11c expression on human peripheral blood leucocyte populations. Human peripheral blood (collected with heparin as the preferred anticoagulant rather than EDTA) was stained with either BD Horizon™ BB515 Mouse IgG1, κ Isotype Control (Cat. No. 564416; Left Plot) or BD Horizon™ BB515 Mouse Anti-Human CD11c antibody (Cat. No. 566835; Right Plot) at 0.5 μg/test. The erythrocytes were lysed with BD FACS™ Lysing Solution (Cat. No. 349202). The two-parameter pseudocolor density plot showing the correlated expression of CD11c (or Ig Isotype control staining) versus side light scatter signals (SSC-A) was derived from gated events with the forward and side light-scatter characteristics of intact leucocyte populations. Flow cytometry and data analysis were performed using a BD LSRFortessa™ X-20 Cell Analyzer System and FlowJo™ software. Data shown in this Technical Data Sheet are not lot specific.

商品详情
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BD Horizon™
ITGAX; integrin alpha X; CD11C; p150 95 integrin alpha chain; SLEB6
Human (QC Testing)
Mouse BALB/c IgG1, κ
Human Synovial Fluid Dendritic Cells
Flow cytometry (Routinely Tested)
0.2 mg/ml
III 256; V S143
AB_2869893
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO


准备和存储

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with BD Horizon™ BB515 under optimum conditions and unconjugated antibody was removed.

推荐的实验流程

       BD™ CompBeads can be used as surrogates to assess fluorescence spillover (Compensation).  When fluorochrome conjugated antibodies are bound to CompBeads, they have spectral properties very similar to cells.   However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls.  It is strongly recommended that when using a reagent for the first time, users compare the spillover on cells and CompBead to ensure that BD Comp beads are appropriate for your specific cellular application.

       For optimal and reproducible results, BD Horizon Brilliant Stain Buffer should be used anytime two or more BD Horizon Brilliant dyes are used in the same experiment.  Fluorescent dye interactions may cause staining artifacts which may affect data interpretation.  The BD Horizon Brilliant Stain Buffer was designed to minimize these interactions.  More information can be found in the Technical Data Sheet of the BD Horizon Brilliant Stain Buffer (Cat. No. 563794/566349) or the BD Horizon Brilliant Stain Buffer Plus (Cat. No. 566385).

       For optimal results, it is recommended to perform 2 washes after staining with antibodies. Cells may be prepared, stained with antibodies and washed twice with wash buffer per established protocols for immunofluorescence staining, prior to acquisition on a flow cytometer. Performing fewer than the recommended wash steps may lead to increased spread of the negative population.

商品通知

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. An isotype control should be used at the same concentration as the antibody of interest.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
  5. BD Horizon Brilliant Stain Buffer is covered by one or more of the following US patents: 8,110,673; 8,158,444; 8,575,303; 8,354,239.
  6. Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
  7. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
566835 Rev. 1
抗体详情
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BU15

The BU15 monoclonal antibody specifically binds to CD11c, which is also known as Integrin alpha X (αX Integrin) or p150,95 Integrin alpha chain. CD11c is a ~150 kDa type I transmembrane glycoprotein that is encoded by ITGAX (Integrin subunit alpha X) which belongs to the integrin alpha chain family. It is variably expressed on monocytes, macrophages, granulocytes, NK cells, dendritic cells, and subsets of B and T cells. CD11c associates with CD18 (Integrin beta 2/β2 Integrin) to form the heterodimeric CD11c:CD18 complex, which is also known as p150,95 Integrin, or the Type 4 Complement Receptor (CR4). CD11c:CD18 binds to fibrinogen, iC3b, ICAM-1 (CD54), or lipopolysaccharide (LPS). CD11c:CD18 functions as an adhesion molecule that mediates cellular binding to ligands expressed on stimulated cells including epithelium and endothelium found during inflammation.

The antibody was conjugated to BD Horizon BB515 which is part of the BD Horizon Brilliant™ Blue family of dyes. With an Ex Max near 490 nm and an Em Max near 515 nm, BD Horizon BB515 can be excited by the blue laser (488 nm) laser and detected with a 530/30 nm filter. This dye has been exclusively developed by BD Biosciences and is up to seven times brighter than FITC with less spillover into the PE channel. Due to similar excitation and emission properties, BB515, FITC, and Alexa Fluor® 488 cannot be used simultaneously. It is not recommended to use BB515 in cocktails that include Streptavidin conjugates as it may cause high background.

566835 Rev. 1
格式详情
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BB515
The BD Horizon Brilliant™ Blue 515 (BB515) dye is part of the BD Horizon Brilliant™ Blue family of dyes. This dye is a polymer fluorochrome with an excitation maximum (Ex Max) at 490-nm and an emission maximum (Em Max) of 515-nm. Driven by BD innovation, BB515 is designed to be excited by the blue laser (488-nm) and detected using an optical filter centered near 520-nm (e.g., 530/30-nm). BB515 reagents are significantly brighter than equivalent FITC or Alexa Fluor™ 488 reagents with less spillover into the PE detector. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
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BB515
Blue 488 nm
490 nm
515 nm
566835 Rev.1
报价单和参考
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研发参考 (4)

  1. Hogg N, Horton MA. Myeloid antigens: New and previously defined clusters. In: McMichael AJ. A.J. McMichael .. et al., ed. Leucocyte typing III : white cell differentiation antigens. Oxford New York: Oxford University Press; 1987:576-602.
  2. Hogg N. Human mononuclear phagocyte molecules and the use of monoclonal antibodies in their detection. Clin Exp Immunol. 1987; 69(3):687-694. (Clone-specific). 查看参考
  3. Luk J, Springer TA. CD11c cluster report. In: Schlossman SF. Stuart F. Schlossman .. et al., ed. Leucocyte typing V : white cell differentiation antigens : proceedings of the fifth international workshop and conference held in Boston, USA, 3-7 November, 1993. Oxford: Oxford University Press; 1995:1590-1592.
  4. Zola H, Swart B, Nicholson I, Voss E. CD11c. In: Zola H. Leukocyte and Stromal Cell Molecules : the CD Markers. Hoboken, N.J.: Wiley-Liss; 2007:60.
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566835 Rev. 1

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