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BD Horizon™ APC-R700 Mouse Anti-Human CD4

Name: APC-R700 Mouse Anti-Human CD4
Brand: APC-R700 Mouse Anti-Human CD4
SKU: 566808

克隆 OKT4 (RUO)

Multiparameter flow cytometric analysis of CD4 expression on human peripheral blood leucocyte populations. Human whole blood was stained with either BD Horizon™ APC-R700 Mouse IgG2b, κ Isotype Control (Cat. No. 565138; Left Plot) or BD Horizon APC-R700 Mouse Anti-Human CD4 antibody (Cat. No. 566808/566809; Right Plot). Erythrocytes were lysed with BD Pharm Lyse™ Lysing Buffer (Cat. No. 555899). The two-parameter pseudocolor density plots showing the correlated expression of CD4 (or Ig Isotype control staining) versus side light-scatter (SSC-A) signals was derived from gated events with the forward and side light-scatter characteristics of viable leucocyte populations. Flow cytometry and data analysis were performed using a BD LSRFortessa™ X-20 Cell Analyzer System and FlowJo™ software.

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实验方案

科学资源

商品详情

Brand: BD Horizon™

Alternative Name: CD4 antigen (p55); T-cell surface antigen T4/Leu-3

Reactivity: Human (QC Testing)

Isotype: Mouse BALB/c x A/J, also known as CAF1 IgG2b, κ

Immunogen: Sheep Erythrocyte Rosette-purified Human T Cells

Application: Flow cytometry (Routinely Tested)

Vol. Per Test: 5 µl

Entrez Gene ID: 920

RRID: AB_2869879

Storage Buffer: Aqueous buffered solution containing BSA, protein stabilizer, glycerol and ≤0.09% sodium azide.

Regulatory Status: RUO

准备和存储

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to the dye under optimum conditions and unconjugated antibody and free dye were removed.

商品通知

This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
An isotype control should be used at the same concentration as the antibody of interest.
Source of all serum proteins is from USDA inspected abattoirs located in the United States.
Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
Alexa Fluor® is a registered trademark of Molecular Probes, Inc., Eugene, OR.
For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.

配套商品

Stain Buffer (FBS) RUO

尺寸 500 ML 品类编号 554656

Stain Buffer (BSA) RUO

尺寸 500 ML 品类编号 554657

Lysing Buffer RUO

尺寸 100 ML 品类编号 555899

Lysing Solution 10X Concentrate CFDA_IVD

尺寸 100 ML 品类编号 349202

APC-R700 Mouse Anti-Human CD4 RUO

尺寸 100 Tests 品类编号 566808

566808 Rev. 1

抗体详情

OKT4

The OKT4 monoclonal antibody specifically recognizes CD4 which is also known as T4 and Leu-3. CD4 is expressed on most thymocytes, T-helper/inducer cells, regulatory T cells (Tregs), and NKT cells. It is also variably expressed on monocytes, macrophages, and some dendritic cells. CD4 is a ~55 kDa type I transmembrane glycoprotein that is encoded by CD4 which belongs to the immunoglobulin superfamily (IgSF). CD4 binds to a nonpolymorphic region of MHC class II and serves as a coreceptor, along with the T cell receptor for antigen (TCR), for the MHC Class II-restricted recognition of antigens on antigen-presenting cells. CD4 also functions as a receptor for human immunodeficiency viruses (HIV). CD4 has four immunoglobulin-like extracellular domains in its extracellular region and a CXCP binding motif for p56Lck in its cytoplasmic domain which regulates the antigen-bound TCR-induced signaling cascade.

This antibody was conjugated to BD Horizon APC-R700, which has been developed exclusively by BD Biosciences as a better alternative to Alexa Fluor® 700. APC-R700 excites and emits at similar wavelengths to Alexa Fluor® 700 yet exhibits significantly improved brightness. This dye can be excited by the red laser and detected with the same filter set as Alexa Fluor® (eg, 730/45-nm filter).

566808 Rev. 1

格式详情

APC-R700

The BD Horizon™ APC-R700 (APC-R700) Dye is a part of the BD APC red family of dyes. This tandem fluorochrome is comprised of an Allophycocyanin (APC) dye donor that has excitation maximum (Ex Max) of 651-nm and an acceptor dye, R700, with an emission maximum (Em Max) at 706-nm. APC-R700, driven by BD innovation, is designed to be excited by the red (627–640-nm) laser and detected using an optical filter centered near 710-nm (e.g., a 720/40-nm bandpass filter). APC-R700 is a brighter alternative to Alexa Fluor™ 700. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.

格式: APC-R700

最多激发源: 651 nm

最多发射源: 706 nm

566808 Rev.1

报价单和参考

View product citations for antibody "566808" on CiteAb

研发参考 (9)

Courtney AH, Lo WL, Weiss A. TCR Signaling: Mechanisms of Initiation and Propagation.. Trends Biochem Sci. 2018; 43(2):108-123. (Biology). 查看参考
Hoffman RA, Kung PC, Hansen WP, Goldstein G.. Simple and rapid measurement of human T lymphocytes and their subclasses in peripheral blood.. Proc Natl Acad Sci U S A. 1980; 77(8):4914-4917. (Clone-specific: Flow cytometry). 查看参考
Horibe K, Knowles RW, Naito K, Morishima Y, Dupont B. Analysis of T lymphocyte antibody specificities: Comparison of serology with immunoprecipitation patterns. In: Bernard A. A. Bernard .. et al., ed. Leucocyte typing : human leucocyte differentiation antigens detected by monoclonal antibodies. Berlin New York: Springer-Verlag; 1984:212-224.
Kung P, Goldstein G, Reinherz EL, Schlossman SF. Monoclonal antibodies defining distinctive human T cell surface antigens.. Science. 1979; 206(4416):347-9. (Immunogen: Cytotoxicity, Flow cytometry, Radioimmunoassay). 查看参考
Miedema F, Terpstra FG, Melief CJM. T Cell-dependent immunoglobulin synthesis in the human system. Studies with T cell-specific monoclonal antibodies. In: Reinherz EL. Ellis L. Reinherz .. et al., ed. Leukocyte typing II. New York: Springer-Verlag; 1986:213-222.
Moebius U. Cluster report: CD4. In: Knapp W. W. Knapp .. et al., ed. Leucocyte typing IV : white cell differentiation antigens. Oxford New York: Oxford University Press; 1989:314-330.
Reinherz EL, Kung PC, Goldstein G, Levey RH, Schlossman SF. Discrete stages of human intrathymic differentiation: analysis of normal thymocytes and leukemic lymphoblasts of T-cell lineage. Proc Natl Acad Sci U S A. 1980; 77(3):1588-1592. (Clone-specific: Cell separation, Cytotoxicity, Flow cytometry). 查看参考
Reinherz EL, Kung PC, Goldstein G, Schlossman SF. Further characterization of the human inducer T cell subset defined by monoclonal antibody.. J Immunol. 1979; 123(6):2894-6. (Clone-specific: Flow cytometry, Fluorescence activated cell sorting). 查看参考
Reinherz EL, Kung PC, Goldstein G, Schlossman SF. Separation of functional subsets of human T cells by a monoclonal antibody.. Proc Natl Acad Sci U S A. 1979; 76(8):4061-5. (Immunogen: Cell separation, Flow cytometry, Fluorescence activated cell sorting). 查看参考

查看所有文件 (9)

566808 Rev. 1

Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.