Skip to main content Skip to navigation
Hamburger Menu
Search icon
Country Selector Country Selector
China (Chinese)
Profile Icon Profile Icon
登录/注册 UserName
产品

解决方案

发现&学习

资源&工具

Support

Search icon Search icon
Close Menu
      Alert Icon
      APC Mouse Anti-NHP CD45
      APC Mouse Anti-NHP CD45
      Flow cytometric analysis of CD45 expression on Rhesus macaque peripheral blood leukocytes. Rhesus macaque whole blood was stained with APC Mouse anti-NHP CD45 antibody (Cat. No. 561290; right Panel) or with a APC Mouse IgG1, κ Isotype Control (Cat. No. 554681; Left Panel). The erythrocytes were lysed with BD PharmLyse™ Lysing Buffer (Cat. No. 555899). Two parameter flow cytometric dot blots showing the correlated expression of CD45 (or Ig Isotype control staining) versus side-scattered light signals were derived from events with the forward light scatter signals of viable leukocytes. Flow cytometry was performed using a BD™ LSR II Flow Cytometer System. 
      APC Mouse Anti-NHP CD45
      Flow cytometric analysis of CD45 expression on Rhesus macaque peripheral blood leukocytes. Rhesus macaque whole blood was stained with APC Mouse anti-NHP CD45 antibody (Cat. No. 561290; right Panel) or with a APC Mouse IgG1, κ Isotype Control (Cat. No. 554681; Left Panel). The erythrocytes were lysed with BD PharmLyse™ Lysing Buffer (Cat. No. 555899). Two parameter flow cytometric dot blots showing the correlated expression of CD45 (or Ig Isotype control staining) versus side-scattered light signals were derived from events with the forward light scatter signals of viable leukocytes. Flow cytometry was performed using a BD™ LSR II Flow Cytometer System. 
      商品详情
      Down Arrow Up Arrow


      BD Pharmingen™
      Pan Leukocyte, NHP-specific; PTPRC; LCA; L-CA; Leukocyte Common Ag
      Rhesus, Cynomolgus, Baboon (QC Testing)
      Mouse IgG1, κ
      Rhesus peripheral whole blood
      Flow cytometry (Routinely Tested)
      5 µl
      AB_10613814
      Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
      RUO


      准备和存储

      Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to APC under optimum conditions, and unconjugated antibody and free APC were removed.
      显示较多信息 blue down arrow 显示较少信息 blue up arrow

      商品通知

      1. This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
      2. An isotype control should be used at the same concentration as the antibody of interest.
      3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
      4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
      5. This APC-conjugated reagent can be used in any flow cytometer equipped with a dye, HeNe, or red diode laser.
      6. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
      7. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
      显示较多信息 blue down arrow 显示较少信息 blue up arrow
      561290 Rev. 2
      抗体详情
      Down Arrow Up Arrow
      D058-1283

      D058-1283 is a CD45 monoclonal antibody specific for non-human primate leucocytes. It was developed using Rhesus peripheral whole blood as the immunogen. It does not cross-react with human leucocytes. This antibody reacts with baboon, Rhesus and Cynomolgus Macaque leucocytes in a similar pattern to CD45 binding to leukocyte common antigen (LCA) on human cells. Immunophenotypic analysis shows that D058-1283 binds to lymphocytes, monocytes and granulocytes of non-human primate blood samples. This antibody is able to block the binding of monoclonal antibody TÜ116; a reported anti-human CD45 antibody that cross-reacts with nonhuman primate leucocytes. In Western blot analysis, the D058-1283 antibody identifies a 180-200 kDa band.

      561290 Rev. 2
      格式详情
      Down Arrow Up Arrow
      APC
      Allophycocyanin (APC), is part of the BD family of phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 651 nm and an emission maximum (Em Max) at 660 nm. APC is designed to be excited by the Red (627-640 nm) laser and detected using an optical filter centered near 660 nm (e.g., a 660/20 nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
      altImg
      APC
      Red 627-640 nm
      651 nm
      660 nm
      561290 Rev.2
      报价单和参考
      Down Arrow Up Arrow

      研发参考 (3)

      1. Kishihara K, Penninger J, Wallace VA, et al. Normal B lymphocyte development but impaired T cell maturation in CD45-exon6 protein tyrosine phosphatase-deficient mice. Cell. 1993; 74(1):143-156. (Biology). 查看参考
      2. Knapp W. W. Knapp .. et al., ed. Leucocyte typing IV : white cell differentiation antigens. Oxford New York: Oxford University Press; 1989:1-1182.
      3. Reimann KA, Waite BC, Lee-Parritz DE, et al. Use of human leukocyte-specific monoclonal antibodies for clinically immunophenotyping lymphocytes of rhesus monkeys. Cytometry. 1994; 17(1):102-108. (Biology). 查看参考
      561290 Rev. 2

      Please refer to Support Documents for Quality Certificates


      Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

      For Research Use Only. Not for use in diagnostic or therapeutic procedures.

      Website Feedback