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Alexa Fluor® 647 Rat anti-Mouse CD267
Alexa Fluor® 647 Rat anti-Mouse CD267

Flow cytometric analysis of Alexa Fluor® 647-conjugated anti-Mouse CD267 on mouse spelenocytes.   Unstimulated mouse splenocytes were pre-incubated with Mouse BD Fc Block™ purified anti-mouse CD16/CD32 mAb 2.4G2 (Cat. No. 553141/553142).  The cells were then stained with FITC-conjugqted anti-mouse CD19 (Cat. No. 553785) and Alexa Fluor® 647-conjugated anti-mouse CD267 antibody (Clone 8F10, Cat. No. 558453; right panel) or Alexa Fluor® -conjugated rat IgG2a (Clone R35-95, Cat. No. 557690; left panel). Flow cytometry was performed and the dot plots were derived from the gated events based on light scattering characteristics of viable splenocytes. Flow cytometry was performed on a BD™ FACSCalibur flow cytometry system.

Flow cytometric analysis of Alexa Fluor® 647-conjugated anti-Mouse CD267 on mouse spelenocytes.   Unstimulated mouse splenocytes were pre-incubated with Mouse BD Fc Block™ purified anti-mouse CD16/CD32 mAb 2.4G2 (Cat. No. 553141/553142).  The cells were then stained with FITC-conjugqted anti-mouse CD19 (Cat. No. 553785) and Alexa Fluor® 647-conjugated anti-mouse CD267 antibody (Clone 8F10, Cat. No. 558453; right panel) or Alexa Fluor® -conjugated rat IgG2a (Clone R35-95, Cat. No. 557690; left panel). Flow cytometry was performed and the dot plots were derived from the gated events based on light scattering characteristics of viable splenocytes. Flow cytometry was performed on a BD™ FACSCalibur flow cytometry system.

商品详情
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BD Pharmingen™
TACI; Taci;Tnfrsf13b; transmembrane activator and CAML interactor
Mouse (QC Testing)
Rat WF, also known as Wistar Furth IgG2a, κ
Mouse TACI Extracellular Domain Protein
Flow cytometry (Routinely Tested)
0.2 mg/ml
57916
AB_647119
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO


准备和存储

The antibody was conjugated to Alexa Fluor® 647 under optimum conditions, and unreacted Alexa Fluor® 647 was removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

推荐的实验流程

For flow cytometry of cell suspensions from peripheral lymphoid tissues, it is recommended that the cells be pre-incubated with Mouse BD Fc Block™ purified anti-mouse CD16/CD32 mAb 2.4G2 (Cat. no.553141/553142).

商品通知

  1. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  2. The Alexa Fluor®, Pacific Blue™, and Cascade Blue® dye antibody conjugates in this product are sold under license from Molecular Probes, Inc. for research use only, excluding use in combination with microarrays, or as analyte specific reagents. The Alexa Fluor® dyes (except for Alexa Fluor® 430), Pacific Blue™ dye, and Cascade Blue® dye are covered by pending and issued patents.
  3. Alexa Fluor® 647 fluorochrome emission is collected at the same instrument settings as for allophycocyanin (APC).
  4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  5. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
  6. Alexa Fluor® is a registered trademark of Molecular Probes, Inc., Eugene, OR.
558453 Rev. 1
抗体详情
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8F10

The 8F10 monoclonal antibody specifically recognizes CD267 which is also known as transmembrane activator and calcium-modulating and cyclophilin ligand interactor(transmembrane activator and CAML interactor or TACI). CD267 is encoded by Tnfrsf13b (tumor necrosis factor receptor superfamily member 13B). CD267 is a type III transmembrane protein receptor that binds to the ligands BAFF (BLyS), and APRIL. BAFF overproduction is associated with lupus erythematosus and Sjögren's disease, two systemic autoimmune diseases. CD267 is expressed most notably on maturing subsets of splenic B cells such as transition type 2 (T2) and marginal zone (MZ) B cells and also in activated T-cells. In CD267 deficient mice, B cell numbers are increased and mice develop autoimmune disorders suggesting that CD267 plays a role in B cell regulation.

558453 Rev. 1
格式详情
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Alexa Fluor™ 647
Alexa Fluor™ 647 Dye is part of the BD red family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 653-nm and an emission maximum (Em Max) at 669-nm. Alexa Fluor 647 is designed to be excited by the Red laser (627-640 nm) and detected using an optical filter centered near 520-nm (e.g., a 660/20 nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
Alexa Fluor™ 647
Red 627-640 nm
653 nm
669 nm
558453 Rev.1
报价单和参考
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研发参考 (4)

  1. Ng LG, Sutherland AP, Newton R, et al. B cell-activating factor belonging to the TNF family (BAFF)-R is the principal BAFF receptor facilitating BAFF costimulation of circulating T and B cells.. J Immunol. 2004; 173(2):807-17. (Biology). 查看参考
  2. Siegel RM, Lenardo MJ.. To B or not to B: TNF family signaling in lymphocytes. Nat Immunol. 2001; 2(7):577-578. (Biology). 查看参考
  3. Yan M, Wang H, Chan B, et al. Activation and accumulation of B cells in TACI-deficient mice.. Nat Immunol. 2001; 2(7):638-43. (Biology). 查看参考
  4. von Bülow GU, Bram RJ. NF-AT activation induced by a CAML-interacting member of the tumor necrosis factor receptor superfamily. Science. 1997; 278(5335):138-141. (Biology). 查看参考
查看所有文件 (4) 查看更少内容
558453 Rev. 1

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Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.