Flow cytometric analysis of ENT2 expression in Hep G2 cells. Cells from the Human Hep G2 [HEPG2] (Hepatocellular carcinoma, ATCC® HB-8065™) cell line were fixed with BD Cytofix™ Fixation Buffer (Cat. No. 554655) and permeabilized with BD Phosflow™ Perm Buffer III (Cat. No. 558050). The cells were then stained with Alexa Fluor™ 647 Rabbit IgG Isotype Control (Cat. No. 569345; dashed line histogram) or Alexa Fluor™ 647 Rabbit Anti-Human ENT2 antibody (Cat. No. 571183; solid line histogram) at 0.03 µg/test. The fluorescence histogram showing ENT2 expression (or Ig Isotype control staining) was derived from gated events with the forward and side light-scatter characteristics of intact cells. Flow cytometry and data analysis were performed using a BD LSRFortessa™ X-20 Cell Analyzer System and FlowJo™ Software. Data shown on this Technical Data Sheet are not lot specific.
Flow cytometric analysis of ENT2 expression in Hep G2 cells. Cells from the Human Hep G2 [HEPG2] (Hepatocellular carcinoma, ATCC® HB-8065™) cell line were fixed with BD Cytofix™ Fixation Buffer (Cat. No. 554655) and permeabilized with BD Phosflow™ Perm Buffer III (Cat. No. 558050). The cells were then stained with Alexa Fluor™ 647 Rabbit IgG Isotype Control (Cat. No. 569345; dashed line histogram) or Alexa Fluor™ 647 Rabbit Anti-Human ENT2 antibody (Cat. No. 571183; solid line histogram) at 0.03 µg/test. The fluorescence histogram showing ENT2 expression (or Ig Isotype control staining) was derived from gated events with the forward and side light-scatter characteristics of intact cells. Flow cytometry and data analysis were performed using a BD LSRFortessa™ X-20 Cell Analyzer System and FlowJo™ Software. Data shown on this Technical Data Sheet are not lot specific.
商品详情
BD Pharmingen™
DER12; ENT2; HNP36; SLC29A2
Human (QC Testing), Rat (Reported)
Rabbit IgG
Not Reported
Intracellular staining (flow cytometry) (Routinely Tested)
0.2 mg/ml
3177
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO
准备和存储
The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to the dye under optimum conditions and unreacted dye was removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
推荐的实验流程
BD® CompBeads can be used as surrogates to assess fluorescence spillover (compensation). When fluorochrome conjugated antibodies are bound to BD® CompBeads, they have spectral properties very similar to cells. However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls. It is strongly recommended that when using a reagent for the first time, users compare the spillover on cell and BD® CompBeads to ensure that BD® CompBeads are appropriate for your specific cellular application.
商品通知
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- An isotype control should be used at the same concentration as the antibody of interest.
- Alexa Fluor® 647 fluorochrome emission is collected at the same instrument settings as for allophycocyanin (APC).
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- This product is provided under an intellectual property license between Life Technologies Corporation and BD Businesses. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The buyer cannot sell or otherwise transfer (a) this product (b) its components or (c) materials made using this product or its components to a third party or otherwise use this product or its components or materials made using this product or its components for Commercial Purposes. Commercial Purposes means any activity by a party for consideration and may include, but is not limited to: (1) use of the product or its components in manufacturing; (2) use of the product or its components to provide a service, information, or data; (3) use of the product or its components for therapeutic, diagnostic or prophylactic purposes; or (4) resale of the product or its components, whether or not such product or its components are resold for use in research. For information on purchasing a license to this product for any other use, contact Life Technologies Corporation, Cell Analysis Business Unit Business Development, 29851 Willow Creek Road, Eugene, OR 97402, USA, Tel: (541) 465-8300. Fax: (541) 335-0504.
- Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
- Alexa Fluor™ is a trademark of Life Technologies Corporation.
- This rabbit monoclonal (RabMabTM) product is, in part, provided under an intellectual property license from Abcam. The purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product for buyer’s internal research use only (whether the buyer is an academic or for-profit entity) and buyer shall not be permitted to reverse engineer or otherwise perform any compositional, structural, functional or other analyses directed to learning the methodology, formulae, sequences, process, make-up or production of this product (or any portion thereof). The sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: (i) in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. If you, as the buyer, do not agree to these conditions, please leave the product unopened and return the contents of this package to BD Biosciences, 10975 Torreyana Road, San Diego, CA 92121. For information on purchasing a license to this product for purposes other than research, contact Abcam at partnerships@abcam.com.
- For U.S. patents that may apply, see bd.com/patents.
配套商品
Stain Buffer (FBS) RUO
尺寸
500 mL
品类编号
554656
Stain Buffer (BSA) RUO
尺寸
500 mL
品类编号
554657
Fixation Buffer RUO
尺寸
100 mL
品类编号
554655
Perm Buffer III RUO
尺寸
125 mL
品类编号
558050
Alexa Fluor™ 647 Rabbit IgG Isotype Control RUO
尺寸
25 µg
品类编号
569345
571183 Rev. 1
抗体详情
EPR11674
The EPR11674 recombinant monoclonal antibody specifically recognizes Equilibrative nucleoside transporter 2 (ENT2), which is also known as Solute carrier family 29 member 2 (SLC29A2). ENT2 is a ~50 kDa glycosylated transport protein involved in the transport of purine and pyrimidine nucleosides and their analogs across cell membranes. ENT2 is expressed in a wide range of tissues, including vascular endothelium, heart, brain, placenta, thymus, pancreas, prostate, and kidney, with particularly high expression in skeletal muscle. ENT2 plays a crucial role in maintaining nucleoside homeostasis, cell proliferation and cell cycle regulation, and is associated with cancer progression. ENT2 is also unique in facilitating hypoxanthine transport, which is required for enhanced DNA synthesis via hypoxanthine recycling.
571183 Rev. 1
格式详情
Alexa Fluor™ 647
Alexa Fluor™ 647 Dye is part of the BD red family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 653-nm and an emission maximum (Em Max) at 669-nm. Alexa Fluor™ 647 is designed to be excited by the Red laser (627-640 nm) and detected using an optical filter centered near 670-nm (e.g., a 660/20 nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
Alexa Fluor™ 647
Red 627-640 nm
653 nm
669 nm
571183 Rev.1
报价单和参考
View product citations for antibody "571183" on CiteAb
研发参考 (4)
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Alarcón S, Garrido W, Vega G, et al. Deficient Insulin-mediated Upregulation of the Equilibrative Nucleoside Transporter 2 Contributes to Chronically Increased Adenosine in Diabetic Glomerulopathy.. Sci Rep. 2017; 7(1):9439. (Clone-specific: Western blot). 查看参考
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Baldwin SA, Beal PR, Yao SY, King AE, Cass CE, Young JD. The equilibrative nucleoside transporter family, SLC29.. Pflugers Arch. 2004; 447(5):735-43. (Biology). 查看参考
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Gandhi R, Cawthorne C, Craggs LJL, et al. Cell proliferation detected using [18F]FLT PET/CT as an early marker of abdominal aortic aneurysm.. J Nucl Cardiol. 2021; 28(5):1961-1971. (Clone-specific). 查看参考
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Naes SM, Ab-Rahim S, Mazlan M, Abdul Rahman A. Equilibrative Nucleoside Transporter 2: Properties and Physiological Roles.. Biomed Res Int. 2020; 2020:5197626. (Biology: Western blot). 查看参考
571183 Rev. 1
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.