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Alexa Fluor® 647 Mouse anti-PLC-γ2 (pY759)
Alexa Fluor® 647 Mouse anti-PLC-γ2 (pY759)
Analysis of PLC-γ2 (pY759) in human Burkitt's lymphoma. Serum-starved Ramos cells were either stimulated (open histogram) by cross-linking of surface IgM with purified Goat anti-Human IgM (SouthernBiotech) at 37°C for 3 minutes or unstimulated (solid histogram).  The cells were fixed with pre-warmed BD Phosflow™ Fix Buffer I (Cat. No. 557870) for 10 minutes at 37ºC, then permeabilized with BD Phosflow™ Perm Buffer III (Cat. No. 558050) on ice for at least 30 minutes, and then stained with Alexa Fluor® 647 Mouse anti-PLC-γ2 (pY759) (Cat. No. 558498).  Flow cytometry was performed on a BD FACSCalibur™ flow cytometry system.
Analysis of PLC-γ2 (pY759) in human Burkitt's lymphoma. Serum-starved Ramos cells were either stimulated (open histogram) by cross-linking of surface IgM with purified Goat anti-Human IgM (SouthernBiotech) at 37°C for 3 minutes or unstimulated (solid histogram).  The cells were fixed with pre-warmed BD Phosflow™ Fix Buffer I (Cat. No. 557870) for 10 minutes at 37ºC, then permeabilized with BD Phosflow™ Perm Buffer III (Cat. No. 558050) on ice for at least 30 minutes, and then stained with Alexa Fluor® 647 Mouse anti-PLC-γ2 (pY759) (Cat. No. 558498).  Flow cytometry was performed on a BD FACSCalibur™ flow cytometry system.
商品详情
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BD Phosflow™
Human (QC Testing), Mouse,Rat (Predicted)
Mouse IgG1, κ
Phosphorylated Human PLC-γ2 Peptide
Intracellular staining (flow cytometry) (Routinely Tested)
20 µl
AB_647139
Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
RUO


准备和存储

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to Alexa Fluor® 647 under optimum conditions, and unreacted Alexa Fluor® 647 was removed.

商品通知

  1. This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
  2. The Alexa Fluor®, Pacific Blue™, and Cascade Blue® dye antibody conjugates in this product are sold under license from Molecular Probes, Inc. for research use only, excluding use in combination with microarrays, or as analyte specific reagents. The Alexa Fluor® dyes (except for Alexa Fluor® 430), Pacific Blue™ dye, and Cascade Blue® dye are covered by pending and issued patents.
  3. Alexa Fluor® 647 fluorochrome emission is collected at the same instrument settings as for allophycocyanin (APC).
  4. Alexa Fluor® is a registered trademark of Molecular Probes, Inc., Eugene, OR.
  5. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
  6. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  7. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
  8. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
558498 Rev. 4
抗体详情
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K86-689.37

The Phospholipase C (PLC) isozymes hydrolyze phosphatidyl inositol biphosphate to inositol triphosphate and diacylglycerol.  The former causes release of calcium from the endoplasmic reticulum, while the latter is an activator of Protein Kinase C.  Within the PLC family, PLC-γ is the only member that contains SH2 and SH3 domains.  These domains enable it to interact with protein tyrosine kinases and become enzymatically activated via phosphorylation.  It exists as two isoforms: PLC-γ1, which is ubiquitously expressed, and PLC-γ2, which is found primarily in the hematopoietic system.  PLC-γ2-null mice exhibit impaired B lymphocyte maturation and defects in Fc receptor functions.  Phosphorylation of PLC-γ2 at tyrosines 753 and 759 (Y759) is required for activation of PLC-γ2 enzyme activity.  PLC-γ2 phosphorylation at Y759 can be induced by stimulation of the B cell receptor in Ramos cells, the collagen receptor in platelets, and the T cell receptor in Jurkat cells, and it occurs downstream of Btk and BLNK in the signaling cascade of activated B cells.  

The K86-689.37.73 antibody recognizes PLC-γ2 phosphorylated at Y759 in the SH2-SH3 linker region.

558498 Rev. 4
格式详情
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Alexa Fluor™ 647
Alexa Fluor™ 647 Dye is part of the BD red family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 653-nm and an emission maximum (Em Max) at 669-nm. Alexa Fluor™ 647 is designed to be excited by the Red laser (627-640 nm) and detected using an optical filter centered near 670-nm (e.g., a 660/20 nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
Alexa Fluor™ 647
Red 627-640 nm
653 nm
669 nm
558498 Rev.4
报价单和参考
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研发参考 (6)

  1. Holodick NE, Tumang JR, Rothstein TL.. Continual signaling is responsible for constitutive ERK phosphorylation in B-1a cells.. Mol Immunol. 2009; 46(15):3029-3036. (Clone-specific: Flow cytometry). 查看参考
  2. Irish JM, Myklebust JH, Alizadeh AA, et al. B-cell signaling networks reveal a negative prognostic human lymphoma cell subset that emerges during tumor progression.. Proc Natl Acad Sci U S A. 2010; 107(29):12747-12754. (Clone-specific: Flow cytometry). 查看参考
  3. Kim YJ, Sekiya F, Poulin B, Bae YS, Rhee SG. Mechanism of B-cell receptor-induced phosphorylation and activation of phospholipase C-γ2. Mol Cell Biol. 2004; 24(22):9986-9999. (Biology).
  4. Ozdener F, Dangelmaier C, Ashby B, Kunapuli SP, Daniel JL. Activation of phospholipase Cγ2 by tyrosine phosphorylation.. Mol Pharmacol. 2002; 62(3):672-679. (Biology).
  5. Wang D, Feng J, Wen R, et al. Phospholipase Cγ2 is essential in the functions of B cell and several Fc receptors. Immunity. 2000; 13:25-35. (Biology).
  6. Wen R, Jou S-T, Chen Y, Hoffmeyer A, Wang D. Phospholipase Cγ2 is essential for specific functions of FcεR and FcγR . J Immunol. 2002; 169:6743-6752. (Biology).
查看所有文件 (6) 查看更少内容
558498 Rev. 4

Please refer to Support Documents for Quality Certificates


Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.