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Alexa Fluor® 647 Mouse Anti-GATA4
Alexa Fluor® 647 Mouse Anti-GATA4

Analysis of Alexa Fluor® 647 Mouse Anti-GATA4 on mouse testis, embryonal carcinoma.  F9 cells were treated with 0.3 µm Retinoic Acid and 1 mm Dibutyryl cAMP for 48 hours  (solid line) or were untreated (dashed line). The cells were fixed (BD Cytofix™ buffer, Cat. No. 554655) for 10 minutes at 37 ºC, permeabilized with BD Perm Buffer™ III (Cat. No. 558050) on ice for 30 minutes. The cells were stained with Alexa Fluor ® 647 Mouse Anti-GATA4, incubated in the dark for 30 minutes at room temperature. This antibody also works in BD Phosflow™ Perm Buffer I and II. Flow cytometry was performed on a BD FACSCanto™ II flow cytometry system.

Analysis of Alexa Fluor® 647 Mouse Anti-GATA4 on mouse testis, embryonal carcinoma.  F9 cells were treated with 0.3 µm Retinoic Acid and 1 mm Dibutyryl cAMP for 48 hours  (solid line) or were untreated (dashed line). The cells were fixed (BD Cytofix™ buffer, Cat. No. 554655) for 10 minutes at 37 ºC, permeabilized with BD Perm Buffer™ III (Cat. No. 558050) on ice for 30 minutes. The cells were stained with Alexa Fluor ® 647 Mouse Anti-GATA4, incubated in the dark for 30 minutes at room temperature. This antibody also works in BD Phosflow™ Perm Buffer I and II. Flow cytometry was performed on a BD FACSCanto™ II flow cytometry system.

商品详情
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BD Pharmingen™
MGC126629, GATA-4
Mouse (QC Testing), Human (Reported)
Mouse BALB/c IgG1, κ
Human GATA4 Peptide
Intracellular staining (flow cytometry) (Routinely Tested)
20 µl
AB_1645185
Aqueous buffered solution containing BSA, protein stabilizer, and ≤0.09% sodium azide.
RUO


准备和存储

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to Alexa Fluor® 647 under optimum conditions, and unreacted Alexa Fluor® 647 was removed.

商品通知

  1. This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
  2. Alexa Fluor® 647 fluorochrome emission is collected at the same instrument settings as for allophycocyanin (APC).
  3. Alexa Fluor® is a registered trademark of Molecular Probes, Inc., Eugene, OR.
  4. The Alexa Fluor®, Pacific Blue™, and Cascade Blue® dye antibody conjugates in this product are sold under license from Molecular Probes, Inc. for research use only, excluding use in combination with microarrays, or as analyte specific reagents. The Alexa Fluor® dyes (except for Alexa Fluor® 430), Pacific Blue™ dye, and Cascade Blue® dye are covered by pending and issued patents.
  5. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
  6. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  7. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
  8. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
560400 Rev. 3
抗体详情
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L97-56

The L97-56 monoclonal antibody reacts with GATA4 (GATA-binding protein 4), a member of the GATA family of zinc finger-containing transcription factors that bind to the GATA nucleotide sequence. This ~50 kDa nuclear protein is expressed in mesodermal and definitive endodermal tissues such as the gastrointestinal tract, gonads, and heart. Genetic studies suggest that GATA4 regulates embryonic cardiac development in mice, and disruption of the GATA4 gene leads to defects in heart tube formation; in humans, GATA4 mutations are associated with atrial septal defects. In the adult heart, GATA4 regulates differentiated gene expression. The roles of GATA4 in endocrine and reproductive functions were recently reviewed.

560400 Rev. 3
格式详情
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Alexa Fluor™ 647
Alexa Fluor™ 647 Dye is part of the BD red family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 653-nm and an emission maximum (Em Max) at 669-nm. Alexa Fluor 647 is designed to be excited by the Red laser (627-640 nm) and detected using an optical filter centered near 520-nm (e.g., a 660/20 nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
Alexa Fluor™ 647
Red 627-640 nm
653 nm
669 nm
560400 Rev.3
报价单和参考
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研发参考 (2)

  1. Oka T, Xu J, Molkentin JD. Re-employment of developmental transcription factors in adult heart disease. Semin Cell Dev Biol. 2007; 18(1):117-131. (Biology). 查看参考
  2. Viger RS, Guittot SM, Anttonen M, Wilson DB, Heikinheimo M. Role of the GATA family of transcription factors in endocrine development, function, and disease. Mol Endocrinol. 2008; 22(4):781-789. (Biology). 查看参考
560400 Rev. 3

Please refer to Support Documents for Quality Certificates


Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.