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PE Mouse Anti-Human Cyclin B1/Isotype Control Reagent Set

PE Mouse Anti-Human Cyclin B1/Isotype Control Reagent Set

(RUO)
PE Mouse Anti-Human Cyclin B1/Isotype Control Reagent Set

Profile of Cyclin B1 in MOLT-4 cells analyzed on a FACSCalibar™ (BDIS, San Jose, CA). Cells were fixed, permeabilized with cold 75% ethano and stained with PE conjugated Cyclin B1 antibody (clone GNS-1, Component No. 51-14545X) or with PE conjugated mouse IgG1 isotype (negative) control antibody (clone MOPC-21, Component. No. 51-13855X-7). Cells were counterstained with 7-AAD (Cat. No. 559925) for DNA content.

Profile of Cyclin B1 in MOLT-4 cells analyzed on a FACSCalibar™ (BDIS, San Jose, CA). Cells were fixed, permeabilized with cold 75% ethano and stained with PE conjugated Cyclin B1 antibody (clone GNS-1, Component No. 51-14545X) or with PE conjugated mouse IgG1 isotype (negative) control antibody (clone MOPC-21, Component. No. 51-13855X-7). Cells were counterstained with 7-AAD (Cat. No. 559925) for DNA content.

商品详情
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BD Pharmingen™
Intracellular staining (flow cytometry) (Routinely Tested)
RUO
AB_393883


描述

Cyclins and cyclin-dependent kinases (cdks) are evolutionarily conserved proteins that are essential for cell-cycle control in eukaryotes. Cyclins (regulatory subunits) bind to cdks (catalytic subunits) to form complexes that regulate the progression of the cell cycle. The main cyclin-cdks complexes formed in vertebrae cells are cyclin-cdks complexes that regulate the progression of the cell cycle. The main cyclin-cdks complexes formed in vertebrae cells are cyclin D-cdk4 (G0/G1), cyclin E-cdk2 (G1/S), cyclin A-cdk2 (S) and cyclin B1-cdk1 (G2/M). These complexes are regulated by activating and inhibitory phosphorylation events, as well as by interactions with small regulatory proteins including p21 and p27[Kip1]. Specific substrates for cdk-cyclin complexes include nuclear lamins, histones, oncogenes (e.g., c-abl and SV40) large T-Ag), tumor suppressor genes (e.g., retinoblastoma protein, Rb) nucleolin and others. Cyclin B1 is a mitotic cyclin complex; expression is normally low in G0/G1, increases in S, and is maximal during G2/M. Cyclin B1 is rapidly degraded at the end of mitosis and is required for cells to exit from mitosis. Clone GNS-1 recognizes an epitope between amino acids 1-21 of human Cyclin B1. GNS-1 crossreacts with hamster and mouse cyclin B1 by western blot analysis; however, it has not been evaluated for crossreactivity by flow cytometry. Recombinant human cyclin B1 was used as immunogen.

准备和存储

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

推荐的实验流程

This reagent set is designed for use in flow cytometry. For flow cytometric analysis, use 20 µl of antibody per test (1x10^6 cells). MOLT-4 human leukemia cells (ATCC CRL-1582) are recommended as a positive control.

商品通知

  1. This antibody has been optimized and preassayed with its matched isotype control to be used at the recommended volume of 20 ul/test. Titration of the reagents or substituting with other (non-matched) isotype control is NOT recommended.
  2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
550783 Rev. 3
组件
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描述 数量/尺寸 零件号 EntrezGene ID
PE Mouse Anti-Human Cyclin B1 100 Tests (1 ea) 51-14545X N/A
PE Mouse IgG1 κ Isotype Control 100 Tests (1 ea) 51-13855X-7 N/A
550783 Rev. 3
报价单和参考
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研发参考 (3)

  1. Cao L, Faha B, Dembski M, Tsai LH, Harlow E, Dyson N. Independent binding of the retinoblastoma protein and p107 to the transcription factor E2F. Nature. 1992; 355(6356):176-179. (Clone-specific: Western blot). 查看参考
  2. Gong J, Traganos F, Darzynkiewicz Z. Discrimination of G2 and mitotic cells by flow cytometry based on different expression of cyclins A and B1. Exp Cell Res. 1995; 220(1):226-231. (Clone-specific). 查看参考
  3. Sherr CJ. Mammalian G1 cyclins. Cell. 1993; 73(6):1059-1065. (Biology). 查看参考
550783 Rev. 3

Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.