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      FITC Mouse Anti-Human p16 Set

      BD Pharmingen™ FITC Mouse Anti-Human p16 Set

      (RUO)
      FITC Mouse Anti-Human p16 Set
      Flow cytometric analysis of p16 expression on WI-26 VA4 human lung cells. Permeabilized WI-26 VA4 cells were stained with either FITC Mouse Anti-Human p16 (Component No. 51-13384X; filled histogram) or FITC Mouse IgG1, κ Isotype Control (Component No. 51-13854X-6; open histogram). Fluorescent histograms were derived from gated events with the side and forward light-scattering characteristics of viable cells. Flow cytometric analysis was performed on a BD FACScan™.
      FITC Mouse Anti-Human p16 Set FITC Mouse Anti-Human p16 Set
      Flow cytometric analysis of p16 expression on WI-26 VA4 human lung cells. Permeabilized WI-26 VA4 cells were stained with either FITC Mouse Anti-Human p16 (Component No. 51-13384X; filled histogram) or FITC Mouse IgG1, κ Isotype Control (Component No. 51-13854X-6; open histogram). Fluorescent histograms were derived from gated events with the side and forward light-scattering characteristics of viable cells. Flow cytometric analysis was performed on a BD FACScan™.
      商品详情
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      BD Pharmingen™
      Human (QC Testing)
      Intracellular staining (flow cytometry) (Routinely Tested)
      RUO
      AB_396464


      描述

      Cyclins and cyclin-dependent kinases (cdks) form active complexes that regulate key events during the progression of the cell cycle and are evolutionarily highly conserved. The p16 protein has been identified as a specific inhibitor of cdk4 because it blocks cdk4 substrate phosphorylation. p16 inhibits cdk4 dependent phosphorylation of the tumor suppressor retinoblastoma protein (Rb) and Rb related proteins, p107 and p130. The biochemical properties of p16 suggest that it may be a tumor suppressor gene product. Recently a gene cloned from the short arm of human chromosome 9, Multiple Tumor Suppressor 1 (MTS1) has been identified as the gene for p16]. The gene, now also known as the CDKN2 gene, has been found to be mutated in a very high percentage of tumors, including 75% of melanoma cell lines.  Alternate names for p16 include p16-INK4, p16-INK4a, ARF, MTS1, CDKN2, CDK4l.

      准备和存储

      Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with FITC under optimum conditions, and unreacted FITC was removed.
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      推荐的实验流程

      Flow cytometry:  For flow cytometry protocols, please refer to "Intracellular Flow" at our website: http://www.bdbiosciences.com/us/s/resources.

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      商品通知

      1. This antibody has been optimized and preassayed with its matched isotype control to be used at the recommended volume of 20 ul/test. Titration of the reagents or substituting with other (non-matched) isotype control is NOT recommended.
      2. An isotype control should be used at the same concentration as the antibody of interest.
      3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
      4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
      5. Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
      6. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
      7. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
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      556560 Rev. 3
      组件
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      描述 数量/尺寸 零件号 EntrezGene ID
      FITC Mouse Anti-Human p16 100 Tests (1 ea) 51-13384X N/A
      FITC Mouse IgG1, κ Isotype Control 100 Tests (1 ea) 51-13854X-6 N/A
      556560 Rev. 3
      报价单和参考
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      研发参考 (7)

      1. Kamb A, Gruis NA, Weaver-Feldhaus J, et al. A cell cycle regulator potentially involved in genesis of many tumor types. Science. 1994; 264(5157):436-440. (Biology). 查看参考
      2. Li Y, Nichols MA, Shay JW, Xiong Y. Transcriptional repression of the D-type cyclin-dependent kinase inhibitor p16 by the retinoblastoma susceptibility gene product pRb. Cancer Res. 1994; 54(23):6078-6082. (Biology). 查看参考
      3. Marx J. Link to hereditary melanoma brightens mood for p16 gene. Science. 1994; 265(5177):1364-1365. (Biology). 查看参考
      4. Serrano M, Hannon GJ, Beach . A new regulatory motif in cell-cycle control causing specific inhibition of cyclin D/CDK4. Nature. 1993; 366(6456):704-707. (Immunogen). 查看参考
      5. Shapiro GI, Edwards CD, Kobzik L, et al. Reciprocal Rb inactivation and p16INK4 expression in primary lung cancers and cell lines. Cancer Res. 1995; 55(3):505-509. (Biology). 查看参考
      6. Tam SW, Shay JW, Pagano M. Differential expression and cell cycle regulation of the cyclin-dependent kinase 4 inhibitor p16Ink4. Cancer Res. 1994; 54(22):5816-5820. (Biology). 查看参考
      7. Yeager T, Stadler W, Belair C, Puthenveettil J, Olopade O, Reznikoff C. Increased p16 levels correlate with pRb alterations in human urothelial cells. Cancer Res. 1995; 55(3):493-497. (Biology). 查看参考
      查看所有文件 (7) 查看更少内容
      556560 Rev. 3

      Please refer to Support Documents for Quality Certificates

      Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

      For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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