Skip to main content Skip to navigation
Hamburger Menu
Search icon
Location Selector Location Selector
China (Chinese)
登录/注册 UserName
Products

Link Arrow
解决方案

Link Arrow
Discover & Learn

Link Arrow
Resources & Tools

Link Arrow
Support

Link Arrow
Close Menu
Website Feedback
Alert Icon
FITC Rat IgG1, κ Isotype Control
商品详情
Down Arrow Up Arrow


BD Pharmingen™
Rat IgG1, κ
Mouse immunoglobulin
Flow cytometry, Intracellular staining (flow cytometry), Isotype control (Routinely Tested)
0.5 mg/ml
AB_395508
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO


准备和存储

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with FITC under optimum conditions, and unreacted FITC was removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
显示较多信息 blue down arrow 显示较少信息 blue up arrow

推荐的实验流程

Recommended Assay Procedure:

The FITC-, PE- and APC-conjugated R3-34 immunoglobulins (Cat. No. 554684; 554685; 554686) are suitable rat IgG1 isotype controls for assessing the level of background staining on paraformaldehyde-fixed/saponin-permeabilized mouse and human cells for flow cytometric analysis. Use at comparable concentrations to antibody of interest (e.g., ≤ 0.5 µg antibody/1 million cells (see image, right panel). For specific methodology, see online protocols or chapter 4 on intracellular staining in Techniques for Immune Function Analysis Application Handbook 1st Edition, both of which are located at our website at www.bdbiosciences.com.  The intracellular cytokine staining technique and use of blocking controls are described in detail by C. Prussin and D. Metcalfe.

显示较多信息 blue down arrow 显示较少信息 blue up arrow

商品通知

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
显示较多信息 blue down arrow 显示较少信息 blue up arrow
554684 Rev. 1
抗体详情
Down Arrow Up Arrow
R3-34

Following immunization of a rat with mouse immunoglobulin (Ig), the Ig from the R3-34 hybridoma was identified as a non-reactive clone. The R3-34 immunoglobulin was selected as an Ig isotype control following screening for low background staining on a variety of mouse and human cells and tissues.

This antibody is routinely tested by flow cytometric analysis. Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.

554684 Rev. 1
格式详情
Down Arrow Up Arrow
FITC
Fluorescein (FITC) is part of the BD blue family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 494-nm and an emission maximum (Em Max) at 518-nm. FITC is designed to be excited by the Blue laser (488-nm) and detected using an optical filter centered near 520 nm (e.g., a 530/30-nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
altImg
FITC
494 nm
518 nm
554684 Rev.1
报价单和参考
Down Arrow Up Arrow
View product citations for antibody "554684" on CiteAb

研发参考 (1)

  1. Prussin C, Metcalfe DD. Detection of intracytoplasmic cytokine using flow cytometry and directly conjugated anti-cytokine antibodies. J Immunol Methods. 1995; 188(1):117-128. (Methodology: IC/FCM Block). 查看参考
554684 Rev. 1

Please refer to Support Documents for Quality Certificates

 

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

 

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.