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PE Mouse Anti-Human CD95
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Mouse C3H, also known as C3H/He, C3H/Bi IgG1, κ
Human CD95-transfected L Cells
Flow cytometry
6.3 μg/mL
20 μL
VI C-64
Phosphate buffered saline with gelatin and 0.1% sodium azide.


Store vials at 2°C–8°C. Conjugated forms should not be frozen. Protect from exposure to light. Each reagent is stable until the expiration date shown on the bottle label when stored as directed.

340480 Rev. 1
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The CD95, clone DX2, is derived from hybridization of mouse Sp2/0 myeloma cells with spleen cells from C3H/He mice immunized with L cells transfected with CD95 antigen.

The CD95 recognizes a 48-kdalton (kDa) antigen (Fas/APO-1) that is a member of the tumor necrosis factor/nerve growth factor (TNF/NGF) superfamily. It is associated with activated cells. Cross linking of CD95 antigen on activated cells can elicit apoptosis.

340480 Rev. 1
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R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
Yellow-Green 488 nm, 532 nm, 561 nm
496 nm, 566 nm
576 nm
340480 Rev.1
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研发参考 (12)

  1. Amasaki Y, Kobayashi S, Takeda T, et al. Up-regulated expression of Fas antigen (CD95) by peripheral naive and memory T cell subsets in patients with systemic lupus erythematosus (SLE): A possible mechanism for lymphopenia. Clin Exptl Immunol. 1995; 99:245-250. (Biology).
  2. Centers for Disease Control. Perspectives in disease prevention and health promotion update: universal precautions for prevention of transmission of human immunodeficiency virus, hepatitis B virus, and other bloodborne pathogens in health-care settings. MMWR. 1988; 37:377-388. (Biology).
  3. Cifone MG, De Maria R, Roncaioli P, et al. Apoptotic signaling through CD95 (Fas/Apo-1) activates an acidic sphingomyelinase. J Exp Med. 1994; 180(4):1547-1552. (Biology). 查看参考
  4. Clinical and Laboratory Standards Institute. 2005. (Biology).
  5. McClosky TW, Oyaizu N, Pahwa S. Review: Apoptosis, HIV infection, and the Fas antigen. Clin Immunol Newsletter. 1995; 15:105-113. (Biology).
  6. Miyawaki T, Uehara T, Nibu R, et al. Differential expression of apoptosisrelated Fas antigen on lymphocyte subpopulations in human peripheral blood. J Immunol. 1992; 149:3753-3758. (Biology).
  7. Möller P, Koretz K, Leithäuser F, et al. Expression of APO-1 (CD95), a member of the NFG/TNF superfamily, in normal and neoplastic colon epithelium. Int J Cancer. 1994; 57:371-377. (Biology).
  8. Oehm A, Behrmann I, Falk W, Pawlita M, et al. Purification and molecular cloning of the APO-1 cell surface antigen, a member of the tumor necrosis factor/nerve growth factor receptor superfamily. J Biol Chem. 1992; 140:197-205. (Biology).
  9. Owen-Schaub L, Angelo L, Radinsky R, Ware C, et al Soluble Fas/APO-1 in tumor cells: A potential regulator of apoptosis? Cancer Letters. 1992; 140:197-205. (Biology).
  10. Owen-Schaub LB, Yonehara S, Crump III WL, Grimm EA. DNA fragmentation and cell death is selectively triggered in activated human lymphocytes by Fas antigen engagement. Cell Immunol. 1992; 140:197-205. (Biology).
  11. Robertson MJ, Ritz J. Knapp W, Dörken B, Gilks W, ed. Leucocyte Typing IV: White Cell Differentiation Antigens. New York, NY: Oxford University Press; 1989:1142-3.
  12. Shima Y, Nishimoto N, Ogata A, Fujii Y, Yoshizaki K, Kishimoto T. Myeloma cells express Fas antigen/APO-1 (CD95) but only some are sensitive to anti-Fas antibody resulting in apoptosis. Blood. 1995; 85:757-764. (Biology).
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340480 Rev. 1

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